FastQCFastQC Report
Thu 26 May 2016
SRR1660578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34500540
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8864842.5694786226534423No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA489480.1418760402011099No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453730.1315138835508082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1233800.079.614448
AGTGGTA1261050.078.195745
GTATCAA1257250.077.987539
GTGGTAT1264750.077.851856
TGGTATC1269500.077.3422857
AAGCAGT1403800.070.925521
GCAGTGG1417700.069.9694443
CAGTGGT1427700.069.347774
AGCAGTG1524450.065.375622
CAACGCA1171350.044.10272212-13
ATCAACG1199850.043.12371410-11
ACGCAGA1228350.041.99395814-15
TCAACGC1175850.041.51535812-13
AGAGTAC1244600.041.18795418-19
AACGCAG1206950.040.4289214-15
GAGTACT1196550.039.55475620-21
CGCAGAG1242100.039.03972216-17
TATCAAC1267150.038.7039410-11
AGTACTT1304400.038.569420-21
GTACTTT1304550.036.4486322-23
Produced by FastQC (version 0.11.5)