FastQCFastQC Report
Thu 26 May 2016
SRR1660577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42027441
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10571992.5154969582849454No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA493560.117437557047549No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT468460.11146526860866927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1423250.079.523298
GTGGTAT1451100.078.1433266
AGTGGTA1455650.078.044185
GTATCAA1451200.077.865589
TGGTATC1461900.077.3879557
AAGCAGT1613850.071.331271
GCAGTGG1609700.071.141823
CAGTGGT1613100.070.704464
AGCAGTG1787650.064.451622
CAACGCA1353450.043.9554312-13
ATCAACG1382550.043.1219910-11
ACGCAGA1399900.042.52116414-15
TCAACGC1349850.041.79564712-13
AACGCAG1377150.040.8706314-15
AGAGTAC1449200.040.83779518-19
CGCAGAG1418650.039.57695416-17
GAGTACT1395350.039.352220-21
TATCAAC1454500.038.82084310-11
AGTACTT1520200.038.32768620-21
GTACTTT1512350.036.35242522-23
Produced by FastQC (version 0.11.5)