FastQCFastQC Report
Thu 26 May 2016
SRR1660577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42027441
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10307672.452604716047308No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT479150.1140088448402081No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA424040.10089598365030125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1460450.079.608268
GTGGTAT1487100.078.548016
AGTGGTA1494450.078.151255
GTATCAA1494900.077.7705469
TGGTATC1498150.077.755467
GCAGTGG1643850.071.386443
AAGCAGT1648850.071.3823551
CAGTGGT1654750.070.879314
AGCAGTG1821750.064.58292
CAACGCA1387300.043.99208512-13
ATCAACG1414850.043.1486510-11
ACGCAGA1435100.042.59203714-15
TCAACGC1383100.041.85376412-13
AGAGTAC1481050.041.02099618-19
AACGCAG1418100.040.7543114-15
CGCAGAG1454650.039.64797216-17
GAGTACT1430700.039.41704620-21
TATCAAC1490150.038.98558410-11
AGTACTT1559700.038.3330620-21
GTACTTT1541150.036.64887222-23
Produced by FastQC (version 0.11.5)