FastQCFastQC Report
Thu 26 May 2016
SRR1660576_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660576_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52950589
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14269162.6948066621128612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA737160.1392165817079013No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT638970.12067287863407902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1909750.080.341648
AGTGGTA1945050.079.1018145
GTGGTAT1949550.078.8499456
GTATCAA1946200.078.631479
TGGTATC1965000.078.089557
AAGCAGT2142750.072.622041
GCAGTGG2178000.071.151413
CAGTGGT2196950.070.285884
AGCAGTG2318650.067.178072
CAACGCA1827900.043.83735712-13
ATCAACG1861400.043.1753510-11
ACGCAGA1916750.041.84333814-15
TCAACGC1828150.041.70926712-13
AGAGTAC1953100.040.83726518-19
AACGCAG1869650.040.71688514-15
GAGTACT1863900.039.57097620-21
TATCAAC1965200.038.93280410-11
CGCAGAG1957750.038.7787416-17
AGTACTT2044550.038.1710220-21
GTACTTT2016600.036.57515322-23
Produced by FastQC (version 0.11.5)