FastQCFastQC Report
Thu 26 May 2016
SRR1660576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52950589
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13901882.625443883164359No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA649270.12261808834647712No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT633130.1195699636126805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1953000.080.155488
AGTGGTA1994700.078.782375
GTATCAA1995300.078.446789
GTGGTAT2009450.078.275396
TGGTATC2019250.077.6931157
AAGCAGT2183300.072.732831
GCAGTGG2225450.071.082793
CAGTGGT2250050.070.1866464
AGCAGTG2372900.066.909292
CAACGCA1881600.043.7029712-13
ATCAACG1914150.043.00761810-11
ACGCAGA1966000.041.9221114-15
TCAACGC1876800.041.55361612-13
AGAGTAC2013100.040.7097118-19
AACGCAG1921400.040.49958814-15
GAGTACT1923300.039.3522120-21
CGCAGAG2004800.038.78434416-17
TATCAAC2019150.038.75892310-11
AGTACTT2100650.038.23824720-21
GTACTTT2071600.036.55249422-23
Produced by FastQC (version 0.11.5)