FastQCFastQC Report
Thu 26 May 2016
SRR1660575_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660575_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40962776
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17955574.38338700482604No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA456180.11136452275597727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2208250.085.228888
TGGTATC2232800.084.0390247
AGTGGTA2238700.083.929065
GTGGTAT2239200.083.8806846
GTATCAA2239600.083.8784569
AAGCAGT2362900.080.406861
CAGTGGT2386600.078.931354
GCAGTGG2419000.078.4781953
AGCAGTG2541200.074.796392
CAACGCA2157250.045.1518612-13
ATCAACG2187100.044.60057410-11
ACGCAGA2204750.044.132714-15
TCAACGC2158850.043.79003512-13
AGAGTAC2218800.043.2249318-19
AACGCAG2184450.043.16343314-15
GAGTACT2166050.042.4150820-21
CGCAGAG2227700.042.22301516-17
TATCAAC2255350.041.6762610-11
AGTACTT2276150.041.57478320-21
GTACTTT2263000.040.65410622-23
Produced by FastQC (version 0.11.5)