##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660575_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 40962776 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937968925738822 34.0 31.0 34.0 30.0 34.0 2 32.089295437399066 34.0 31.0 34.0 30.0 34.0 3 32.10974866547131 34.0 31.0 34.0 30.0 34.0 4 35.52835484099027 37.0 35.0 37.0 33.0 37.0 5 35.5213318550481 37.0 35.0 37.0 33.0 37.0 6 35.53839703149025 37.0 35.0 37.0 33.0 37.0 7 35.48954289621387 37.0 35.0 37.0 33.0 37.0 8 35.508935283097024 37.0 35.0 37.0 33.0 37.0 9 37.17749466002988 39.0 37.0 39.0 34.0 39.0 10-11 37.096820232593615 39.0 37.0 39.0 34.0 39.0 12-13 37.126049745749654 39.0 37.0 39.0 34.0 39.0 14-15 38.51657819772761 40.0 38.0 41.0 34.0 41.0 16-17 38.47465055835083 40.0 38.0 41.0 34.0 41.0 18-19 38.43979055521042 40.0 38.0 41.0 34.0 41.0 20-21 38.380855535767395 40.0 38.0 41.0 34.0 41.0 22-23 38.234434953822465 40.0 38.0 41.0 33.5 41.0 24-25 38.23047385753348 40.0 38.0 41.0 34.0 41.0 26-27 38.20593387030215 40.0 38.0 41.0 34.0 41.0 28-29 38.138392012299164 40.0 38.0 41.0 33.0 41.0 30-31 38.0520759872329 40.0 38.0 41.0 33.0 41.0 32-33 37.94591036750049 40.0 38.0 41.0 33.0 41.0 34-35 37.84624075526522 40.0 38.0 41.0 33.0 41.0 36-37 37.682862081905775 40.0 38.0 41.0 33.0 41.0 38-39 37.3365135580655 40.0 36.5 41.0 32.0 41.0 40-41 37.14325302318378 40.0 36.0 41.0 32.0 41.0 42-43 37.046595633557644 40.0 36.0 41.0 31.5 41.0 44-45 36.92107046163082 40.0 36.0 41.0 31.0 41.0 46-47 36.77091751545354 40.0 35.0 41.0 31.0 41.0 48-49 36.57349453074176 39.0 35.0 41.0 30.5 41.0 50-51 35.477965983067165 38.0 34.0 40.0 29.0 40.5 52-53 35.371045629329416 38.0 35.0 39.5 28.5 40.5 54-55 35.77764932239944 39.0 35.0 40.5 28.5 41.0 56-57 35.672610530594895 39.0 35.0 41.0 28.0 41.0 58-59 35.49521675484103 39.0 35.0 41.0 28.0 41.0 60-61 35.2744454501814 39.0 35.0 41.0 27.0 41.0 62-63 35.0095614491557 38.5 35.0 41.0 26.5 41.0 64-65 34.70401124669871 38.0 35.0 40.5 26.0 41.0 66-67 34.388807938700246 37.0 35.0 40.0 26.0 41.0 68-69 34.047586899383965 37.0 34.0 40.0 26.0 41.0 70-71 33.685788092096104 36.0 34.0 39.0 26.0 41.0 72-73 33.311447776390935 35.5 34.0 39.0 25.0 41.0 74-75 32.92222507087899 35.0 34.0 38.5 24.0 40.0 76-77 32.545906764229066 35.0 33.5 37.0 23.0 39.5 78-79 32.18499804554261 35.0 33.0 37.0 22.5 39.0 80-81 31.838274486084636 35.0 33.0 36.0 20.5 39.0 82-83 31.543081760376786 35.0 33.0 36.0 20.0 37.0 84-85 31.27879189633046 35.0 33.0 35.5 18.5 37.0 86-87 31.034372719270785 35.0 33.0 35.0 17.5 36.5 88-89 30.81525089998783 35.0 33.0 35.0 13.0 36.0 90-91 30.617188627548096 35.0 32.0 35.0 7.5 36.0 92-93 30.427138434172527 35.0 32.0 35.0 2.0 36.0 94-95 30.244222364226488 35.0 32.0 35.0 2.0 35.5 96-97 30.045358839937997 35.0 32.0 35.0 2.0 35.0 98-99 29.867667354868722 35.0 32.0 35.0 2.0 35.0 100 29.73564650501226 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3658470021943536 1101 2 0.34123874673084487 1101 3 0.33393008281969117 1101 4 0.28000942128757345 1101 5 0.2952017107391214 1101 6 0.31172208710766824 1101 7 0.2760411398837235 1101 8 0.26880112195544115 1101 9 0.3925073672497277 1101 10-11 0.39700685073006525 1101 12-13 0.40905026093329866 1101 14-15 0.46940954338406016 1101 16-17 0.45107359174165396 1101 18-19 0.48007800173407134 1101 20-21 0.47024131286524806 1101 22-23 0.45634571216901776 1101 24-25 0.4380044419673723 1101 26-27 0.39991807361639786 1101 28-29 0.4667907754048102 1101 30-31 0.4449782490084715 1101 32-33 0.43641394346153817 1101 34-35 0.4766137937913655 1101 36-37 0.42972564890988707 1101 38-39 0.4341465596557086 1101 40-41 0.5592624397543773 1101 42-43 0.47358327924635546 1101 44-45 0.45080982651212054 1101 46-47 0.4511918792900076 1101 48-49 0.5055531369191684 1101 50-51 0.3856783440396896 1101 52-53 0.4252354954983133 1101 54-55 0.5105830123799038 1101 56-57 0.5058151420821204 1101 58-59 0.49236046945141965 1101 60-61 0.49057582868776706 1101 62-63 0.4680392186857816 1101 64-65 0.4449538480051274 1101 66-67 0.48059984474019046 1101 68-69 0.46929219734482075 1101 70-71 0.46974769465099797 1101 72-73 0.41826789195058467 1101 74-75 0.44491044241470945 1101 76-77 0.4205724349057789 1101 78-79 0.44100119690327944 1101 80-81 0.42044888854754703 1101 82-83 0.39786068637238614 1101 84-85 0.35106357642567687 1101 86-87 0.3831662512692624 1101 88-89 0.36121070967668345 1101 90-91 0.3570778242150503 1101 92-93 0.34127304454263196 1101 94-95 0.2819707839964778 1101 96-97 0.3345304424003466 1101 98-99 0.390509168977033 1101 100 0.3785998367687 1102 1 0.4187865072506547 1102 2 0.43005723603879176 1102 3 0.44036158052539065 1102 4 0.38638477521130454 1102 5 0.3759952948761125 1102 6 0.38717257218175405 1102 7 0.392817194602749 1102 8 0.39344272311331707 1102 9 0.43260586720770533 1102 10-11 0.4787310482509852 1102 12-13 0.4872244856832424 1102 14-15 0.5026817980720537 1102 16-17 0.5255827758395313 1102 18-19 0.5684952926098674 1102 20-21 0.571492922775299 1102 22-23 0.5613766803256723 1102 24-25 0.5798104121005849 1102 26-27 0.5565909369049038 1102 28-29 0.5601568977497635 1102 30-31 0.5474016814425298 1102 32-33 0.5334012838714628 1102 34-35 0.5570350149766838 1102 36-37 0.5420027487567722 1102 38-39 0.5416203304099199 1102 40-41 0.6626095467521793 1102 42-43 0.5996696103442289 1102 44-45 0.5772805916610295 1102 46-47 0.5642958028831302 1102 48-49 0.5633685094091376 1102 50-51 0.49826694050803866 1102 52-53 0.529380234242737 1102 54-55 0.6471144202630086 1102 56-57 0.6677575976375678 1102 58-59 0.6428929877160314 1102 60-61 0.6199935969692945 1102 62-63 0.6021498829276553 1102 64-65 0.5963619187320575 1102 66-67 0.5449403342000991 1102 68-69 0.5729566303180036 1102 70-71 0.5190794681758675 1102 72-73 0.5094985737617179 1102 74-75 0.5116674451768688 1102 76-77 0.48885899255612486 1102 78-79 0.5423901305344359 1102 80-81 0.4965808812327097 1102 82-83 0.4430581496035835 1102 84-85 0.45374558899556305 1102 86-87 0.44367044052340887 1102 88-89 0.4302665318571144 1102 90-91 0.37831307500513844 1102 92-93 0.36499534621186314 1102 94-95 0.40219280368035726 1102 96-97 0.4235465816597461 1102 98-99 0.4474823740950171 1102 100 0.44225061867742 1103 1 0.3826159579447257 1103 2 0.3425732633894256 1103 3 0.36895770806079753 1103 4 0.3437633304098213 1103 5 0.3456349786557311 1103 6 0.34078923029054664 1103 7 0.34942054233943765 1103 8 0.3754848542431617 1103 9 0.4130735230643552 1103 10-11 0.39624982036647793 1103 12-13 0.4160713376518572 1103 14-15 0.46974517687323925 1103 16-17 0.48908587473591325 1103 18-19 0.5370393364251953 1103 20-21 0.5196373355917387 1103 22-23 0.4814579082947503 1103 24-25 0.483180170416027 1103 26-27 0.47107245434160205 1103 28-29 0.4753293982121889 1103 30-31 0.4675362403236605 1103 32-33 0.42984967062523793 1103 34-35 0.4907928868575695 1103 36-37 0.49997249356529494 1103 38-39 0.5305525453981232 1103 40-41 0.5831781652615291 1103 42-43 0.5441322413534451 1103 44-45 0.5479065780065397 1103 46-47 0.48147545531637803 1103 48-49 0.5529879528105823 1103 50-51 0.4530000221779531 1103 52-53 0.4757186344135036 1103 54-55 0.59753125668 1103 56-57 0.6056593527341434 1103 58-59 0.5803226239659196 1103 60-61 0.5991835218637434 1103 62-63 0.6062424876059609 1103 64-65 0.6132715592547555 1103 66-67 0.5957245201405215 1103 68-69 0.5774181691644102 1103 70-71 0.5585193138182589 1103 72-73 0.5783264385338995 1103 74-75 0.5998542174212105 1103 76-77 0.536712651705443 1103 78-79 0.5708170459432793 1103 80-81 0.5471162321578191 1103 82-83 0.5450269151210883 1103 84-85 0.5522079824754407 1103 86-87 0.5155262582247602 1103 88-89 0.4953123215254074 1103 90-91 0.5065212306153732 1103 92-93 0.49901087655750587 1103 94-95 0.5263698553849352 1103 96-97 0.5679784398210295 1103 98-99 0.5892673934140689 1103 100 0.6087094185461268 1104 1 0.25609061037753733 1104 2 0.2799536618845906 1104 3 0.26076355282609853 1104 4 0.225371481286615 1104 5 0.2659556595200172 1104 6 0.2649956272199958 1104 7 0.28628067034470916 1104 8 0.2826943085674287 1104 9 0.3612069160619171 1104 10-11 0.35876562765265163 1104 12-13 0.3640952771109909 1104 14-15 0.41810045399703455 1104 16-17 0.38172065096474483 1104 18-19 0.35278680791404327 1104 20-21 0.3373605367441783 1104 22-23 0.35555017703082115 1104 24-25 0.35955843235493745 1104 26-27 0.3503938667837829 1104 28-29 0.40512681550449514 1104 30-31 0.4052257196463174 1104 32-33 0.3621024891549425 1104 34-35 0.40697379213064266 1104 36-37 0.39055489794183273 1104 38-39 0.4239091272556763 1104 40-41 0.5208366201691348 1104 42-43 0.42606738294453095 1104 44-45 0.43239494361083075 1104 46-47 0.4352484902915066 1104 48-49 0.4243308450550174 1104 50-51 -0.003910577492270306 1104 52-53 0.2407263348293469 1104 54-55 0.3662224305102626 1104 56-57 0.3831325123795679 1104 58-59 0.41102794888896454 1104 60-61 0.3973353395009127 1104 62-63 0.3951509860553344 1104 64-65 0.36383948051341264 1104 66-67 0.36588330387724 1104 68-69 0.311233150240092 1104 70-71 0.2438927721030879 1104 72-73 0.27915633076124635 1104 74-75 0.26717379878448355 1104 76-77 0.26797592990091346 1104 78-79 0.2725677531171229 1104 80-81 0.27413463663457094 1104 82-83 0.2908575163902931 1104 84-85 0.26632285093991115 1104 86-87 0.2703576374637713 1104 88-89 0.2927291633070901 1104 90-91 0.23655333514413357 1104 92-93 0.2628106965588586 1104 94-95 0.30799090568099174 1104 96-97 0.2565329019878355 1104 98-99 0.3210980958120011 1104 100 0.2498362933517022 1105 1 0.22400708782723555 1105 2 0.2371705125807253 1105 3 0.21539668209726415 1105 4 0.20442751117477798 1105 5 0.22169603010397765 1105 6 0.17900346125892241 1105 7 0.20139780778357164 1105 8 0.20972219805716463 1105 9 0.2488417349573453 1105 10-11 0.24867464104529802 1105 12-13 0.22487582241094373 1105 14-15 0.2836636378718751 1105 16-17 0.3202732822443082 1105 18-19 0.3250911692640841 1105 20-21 0.31073786253771374 1105 22-23 0.31592040833908186 1105 24-25 0.28066293760901573 1105 26-27 0.29558477278808226 1105 28-29 0.2819417663772086 1105 30-31 0.29871006425702973 1105 32-33 0.31695166350601056 1105 34-35 0.29640707721411985 1105 36-37 0.3397266269715331 1105 38-39 0.3347718791112797 1105 40-41 0.38502732634927384 1105 42-43 0.3124722213375648 1105 44-45 0.32739762931183236 1105 46-47 0.3210740307457698 1105 48-49 0.31649080353835046 1105 50-51 0.2831649494318711 1105 52-53 0.33488394662907695 1105 54-55 0.4020127996894587 1105 56-57 0.38194688570004587 1105 58-59 0.35285630744958496 1105 60-61 0.33166989945192427 1105 62-63 0.327939333578378 1105 64-65 0.3104603123224976 1105 66-67 0.3013880309450414 1105 68-69 0.3212970965031374 1105 70-71 0.29008035892942985 1105 72-73 0.2990810945067963 1105 74-75 0.33838826221308693 1105 76-77 0.2909374391040984 1105 78-79 0.28046006884743946 1105 80-81 0.30472919556120814 1105 82-83 0.2907773250900014 1105 84-85 0.3098255509009249 1105 86-87 0.29247093280318737 1105 88-89 0.24941661153504668 1105 90-91 0.2573317992452395 1105 92-93 0.23780265790431088 1105 94-95 0.26168546925467595 1105 96-97 0.2539455270750288 1105 98-99 0.23025589885729403 1105 100 0.265516617176182 1106 1 0.16387712041531088 1106 2 0.16695413507878953 1106 3 0.13407491253306603 1106 4 0.12188521437585109 1106 5 0.1280453724676036 1106 6 0.1518048650105044 1106 7 0.18072842119993737 1106 8 0.19016061670829743 1106 9 0.1924589313127214 1106 10-11 0.2353932879102416 1106 12-13 0.21849410223629206 1106 14-15 0.22619585781631457 1106 16-17 0.18461431772203696 1106 18-19 0.23168309435308743 1106 20-21 0.2332326208074278 1106 22-23 0.2609593768763929 1106 24-25 0.22168193483704357 1106 26-27 0.2125417953314539 1106 28-29 0.23833825038691003 1106 30-31 0.17738448541115304 1106 32-33 0.24347061955192117 1106 34-35 0.20845247819666923 1106 36-37 0.2710767029021852 1106 38-39 0.28765860991099856 1106 40-41 0.3475987722394649 1106 42-43 0.26762108487699976 1106 44-45 0.20330364329156936 1106 46-47 0.2504913831200426 1106 48-49 0.215944469903107 1106 50-51 0.16290913502407278 1106 52-53 0.21120919291635687 1106 54-55 0.18708179340833908 1106 56-57 0.22389757545094824 1106 58-59 0.19529907346949216 1106 60-61 0.2293994306613314 1106 62-63 0.24821181152599792 1106 64-65 0.2037440297363986 1106 66-67 0.19994228686028492 1106 68-69 0.21532629366560485 1106 70-71 0.17675338784280825 1106 72-73 0.11811093974697684 1106 74-75 0.14534726445016588 1106 76-77 0.15384099163127019 1106 78-79 0.1948273584941589 1106 80-81 0.21177322418090228 1106 82-83 0.17027070561062985 1106 84-85 0.18237860704183007 1106 86-87 0.23395783676012627 1106 88-89 0.2537514160356089 1106 90-91 0.2506347130305535 1106 92-93 0.22853283222553245 1106 94-95 0.2511189141027792 1106 96-97 0.22133076440050203 1106 98-99 0.20840551508793936 1106 100 0.2043910132488591 1107 1 0.14794859367846058 1107 2 0.09303327814128437 1107 3 0.1450657584462718 1107 4 0.16018357179986253 1107 5 0.14756488725523553 1107 6 0.14838766448264096 1107 7 0.0944163223514991 1107 8 0.12636811144574267 1107 9 0.1797619969184936 1107 10-11 0.1727398133377278 1107 12-13 0.15627940057993328 1107 14-15 0.2140671261818241 1107 16-17 0.20525232709440644 1107 18-19 0.18698066202596664 1107 20-21 0.2322513869007352 1107 22-23 0.25074727086049364 1107 24-25 0.2088862108380809 1107 26-27 0.21785115716845382 1107 28-29 0.2324090352514645 1107 30-31 0.1712965885757214 1107 32-33 0.21679161238575517 1107 34-35 0.17162862761511377 1107 36-37 0.19497311925537275 1107 38-39 0.19090546706574685 1107 40-41 0.23587736631964162 1107 42-43 0.23400417930614736 1107 44-45 0.211104370859438 1107 46-47 0.20610739815415968 1107 48-49 0.2132965309278987 1107 50-51 0.22894045321013579 1107 52-53 0.24555879930191793 1107 54-55 0.26909943891739374 1107 56-57 0.2813123139016014 1107 58-59 0.3021865679833624 1107 60-61 0.30436840388767905 1107 62-63 0.28823413693769595 1107 64-65 0.26257860307972436 1107 66-67 0.2431068918139303 1107 68-69 0.2681298186731311 1107 70-71 0.2653778968257612 1107 72-73 0.27038180902247433 1107 74-75 0.28785327234708546 1107 76-77 0.2510988996201675 1107 78-79 0.29003496699515807 1107 80-81 0.2432102177059292 1107 82-83 0.2389641969273022 1107 84-85 0.2541068971941165 1107 86-87 0.1832591808948969 1107 88-89 0.25077919608952826 1107 90-91 0.26039994877323736 1107 92-93 0.24696351901248548 1107 94-95 0.24493818328722128 1107 96-97 0.21362040863695952 1107 98-99 0.2585762571665242 1107 100 0.27950902375460984 1108 1 0.0979977726408201 1108 2 0.11390766237667549 1108 3 0.1157820613306626 1108 4 0.10824490399622988 1108 5 0.06692038905137565 1108 6 0.13749355971884114 1108 7 0.1320033890496788 1108 8 0.08310367097361393 1108 9 0.16212994569593064 1108 10-11 0.1528651671884802 1108 12-13 0.1834649929394203 1108 14-15 0.22958363257419734 1108 16-17 0.2323423410454808 1108 18-19 0.17738067763022514 1108 20-21 0.2528125623367643 1108 22-23 0.22041766478849212 1108 24-25 0.2146601733081681 1108 26-27 0.2062463909324066 1108 28-29 0.1622447115040373 1108 30-31 0.1938180266077012 1108 32-33 0.20146807729275196 1108 34-35 0.21372585888308748 1108 36-37 0.17661174434098115 1108 38-39 0.21032995032864932 1108 40-41 0.20772533242134017 1108 42-43 0.20584267118596955 1108 44-45 0.21009042129636413 1108 46-47 0.22279229150695556 1108 48-49 0.2086462427635709 1108 50-51 0.13577465602218552 1108 52-53 0.1494308851600863 1108 54-55 0.24742479293763608 1108 56-57 0.22308024238565594 1108 58-59 0.2142672396985219 1108 60-61 0.19265662641787173 1108 62-63 0.17633047541684022 1108 64-65 0.1738790847728069 1108 66-67 0.21304994121674525 1108 68-69 0.1868683857396931 1108 70-71 0.24177977425340913 1108 72-73 0.20494284026839438 1108 74-75 0.20005116021100378 1108 76-77 0.11527892071981682 1108 78-79 0.16981350852164212 1108 80-81 0.20349985362434353 1108 82-83 0.2583659837374732 1108 84-85 0.23890597442582973 1108 86-87 0.20425916681140066 1108 88-89 0.19328497186561577 1108 90-91 0.2760300778030391 1108 92-93 0.29527139687077764 1108 94-95 0.29763307063114297 1108 96-97 0.2989557065346098 1108 98-99 0.32654022581496633 1108 100 0.33849530605328226 1109 1 0.07911230562963922 1109 2 0.052869565700582655 1109 3 0.07570540998583652 1109 4 0.06309018802413391 1109 5 0.05445303473695873 1109 6 0.03332722831830637 1109 7 0.06397718795065543 1109 8 0.08953075855020387 1109 9 0.12652225236649173 1109 10-11 0.13888218128035135 1109 12-13 0.1060925031102613 1109 14-15 0.13524058063342892 1109 16-17 0.10725209432995086 1109 18-19 0.12723179148741792 1109 20-21 0.12926860424119724 1109 22-23 0.1114659418676851 1109 24-25 0.10080984954826278 1109 26-27 0.10688670028678615 1109 28-29 0.13127420323730377 1109 30-31 0.1312334728196234 1109 32-33 0.08009253724024035 1109 34-35 0.12348971409460319 1109 36-37 0.06636189790263103 1109 38-39 0.055728944212624754 1109 40-41 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2316 54-55 -1.219753739906949 2316 56-57 -1.1895986715230507 2316 58-59 -1.1505984969030436 2316 60-61 -1.1260484113988625 2316 62-63 -1.1231464146963859 2316 64-65 -1.0389337209813476 2316 66-67 -1.0945593737735422 2316 68-69 -1.1636813368806642 2316 70-71 -1.0696935524589222 2316 72-73 -1.1228429369422983 2316 74-75 -1.0982904095345134 2316 76-77 -1.047025491792951 2316 78-79 -1.02572804989083 2316 80-81 -1.0595523126524995 2316 82-83 -1.0584130966599155 2316 84-85 -1.0635168551009144 2316 86-87 -1.0618323823853864 2316 88-89 -1.0787001157915874 2316 90-91 -1.0773918104534097 2316 92-93 -1.07616146806156 2316 94-95 -1.0543146083158348 2316 96-97 -1.0632026885643349 2316 98-99 -1.030157180458346 2316 100 -1.0855784539881022 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 300830.0 3 44097.0 4 36962.0 5 37275.0 6 37950.0 7 40673.0 8 46527.0 9 58163.0 10 69643.0 11 80212.0 12 85211.0 13 88090.0 14 85752.0 15 82828.0 16 96663.0 17 121832.0 18 165651.0 19 248407.0 20 463454.0 21 557766.0 22 257947.0 23 213748.0 24 236179.0 25 266916.0 26 295923.0 27 342382.0 28 408326.0 29 495616.0 30 608387.0 31 760231.0 32 969625.0 33 1262042.0 34 1731894.0 35 2621552.0 36 4480602.0 37 8621194.0 38 1.1120432E7 39 3511121.0 40 10673.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.666248782571795 33.64652830723666 26.971956591844094 22.715266318347457 2 21.891283676022667 31.477173104280666 27.501093533518084 19.130449686178586 3 25.00499600136775 25.880358921039498 26.505229595713924 22.609415481878827 4 20.44110625437221 27.279561625844373 25.73685850736485 26.542473612418565 5 21.971315026170785 31.054039573247188 25.967822918620982 21.00682248196104 6 26.508269345512748 26.345050355314285 26.22891526850471 20.917765030668257 7 21.459128506026794 26.473738232681065 30.866560590474744 21.2005726708174 8 25.762864057907713 26.167169734532266 26.485770566629753 21.58419564093027 9 25.702191007575202 25.611262898032955 27.05349052831785 21.633055566073992 10-11 21.127812565987536 28.841266600355535 28.724942985669312 21.305977847987613 12-13 21.186251319460187 26.43460158985873 28.546813354871954 23.832333735809126 14-15 21.196079688285124 31.35651073374533 26.078128509518006 21.36928106845154 16-17 23.753032296215597 26.317625087571656 26.086308822352887 23.84303379385986 18-19 21.32108777898337 28.766298162103315 26.031275049363504 23.881339009549805 20-21 23.827507025757278 28.768694516614556 25.98904266226895 21.414755795359213 22-23 23.78100444496197 26.39620742231085 28.434312406330207 21.388475726396972 24-25 21.414750847343676 28.703416360213208 25.867414364447207 24.014418427995913 26-27 21.451211045006435 26.258699284604166 30.832360089951884 21.45772958043752 28-29 21.464889715345322 26.345361870739215 30.79725705366369 21.39249136025177 30-31 21.46171627032921 26.303251663881692 30.75126649499659 21.483765570792507 32-33 21.50987022339148 26.34925192116166 30.663415710101187 21.477462145345672 34-35 21.505798016325176 26.447801739113746 30.608827756920576 21.4375724876405 36-37 21.491410167168368 26.41040272131469 30.596080313507727 21.502106798009216 38-39 21.519835558733185 26.383227359944993 30.62123226183858 21.47570481948324 40-41 21.606951595614206 26.438816726420576 30.56824139578523 21.385990282179986 42-43 21.547033647720376 26.460802868544135 30.524237641370654 21.46792584236483 44-45 21.589803918227314 26.475790807128774 30.517117545586608 21.4172877290573 46-47 21.58789971680092 26.586961904813638 30.46860386742311 21.35653451096233 48-49 21.68041112630467 26.568237699061736 30.356295083128366 21.395056091505225 50-51 21.672909762492164 26.617067373284332 30.330919155066745 21.379103709156762 52-53 21.767953044211154 26.770850112052347 30.121882649295422 21.339314194441076 54-55 22.05592955563078 26.977397678083943 29.50255799449423 21.464114771791053 56-57 22.41117389962891 27.37410958609945 28.65833971501115 21.556376799260494 58-59 22.518052130865424 27.7811949905301 28.175485388976945 21.525267489627534 60-61 22.60027098707748 27.957721034936974 27.913969019427086 21.52803895855846 62-63 22.66881418743745 28.068579844965175 27.805541553889633 21.457064413707734 64-65 22.783677183095552 28.121997450765424 27.76493345036675 21.32939191577227 66-67 22.88680661328414 27.970064024554457 27.86516852012212 21.277960842039285 68-69 22.999095827711454 27.82352297204073 28.027136301738558 21.15024489850926 70-71 23.134267599406126 27.756767823027666 28.121399107232488 20.98756547033372 72-73 23.289576531258064 27.705686534418234 28.07197545334069 20.932761480983014 74-75 23.51485419863396 27.73673520854265 27.955802938145162 20.79260765467823 76-77 23.749943552944153 27.881650737396015 27.669334117384313 20.69907159227552 78-79 24.005332971939225 27.984281846583098 27.344508563930713 20.66587661754696 80-81 24.22952630580015 28.101644409353515 27.10092312160973 20.567906163236604 82-83 24.40451958845927 28.245660243197186 26.87474935216706 20.475070816176487 84-85 24.618627223052926 28.25938949787269 26.694479289675865 20.42750398939852 86-87 24.82234610279164 28.260716756554466 26.58712050629371 20.32981663436019 88-89 25.007406200005406 28.301389264806026 26.45253780964749 20.23866672554108 90-91 25.199038367397563 28.25617964799061 26.315629423037528 20.2291525615743 92-93 25.37685829176016 28.235438328427946 26.25641170758098 20.13129167223091 94-95 25.54984784855543 28.223840269451472 26.209088576761157 20.01722330523194 96-97 25.704207874348107 28.150181530974823 26.115751818965983 20.02985877571109 98-99 25.850295307471566 28.092899552170604 26.10516730179471 19.951637838563116 100 25.927177529568525 28.124559836856573 26.029797724854774 19.91846490872013 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 67677.0 1 43021.5 2 14881.5 3 11022.5 4 10398.0 5 10118.0 6 10389.0 7 10613.0 8 10560.5 9 10653.5 10 11193.0 11 14207.5 12 173225.0 13 208461.5 14 84679.5 15 86029.0 16 94228.0 17 103007.0 18 111700.0 19 121266.0 20 133283.5 21 151480.5 22 177524.0 23 207388.0 24 238393.0 25 276391.0 26 323839.0 27 377502.0 28 435046.0 29 490627.0 30 542046.0 31 594593.0 32 654429.0 33 715563.0 34 776554.5 35 846147.0 36 917997.0 37 975976.0 38 1041220.5 39 1108042.0 40 1157521.5 41 1216380.5 42 1279109.0 43 1310755.5 44 1327162.5 45 1362112.5 46 1400376.0 47 1425409.5 48 1446000.5 49 1463391.0 50 1466370.5 51 1459733.0 52 1444766.5 53 1407338.5 54 1353364.5 55 1288455.5 56 1207263.0 57 1104180.5 58 978124.0 59 836865.5 60 685901.0 61 545839.0 62 424319.0 63 318420.5 64 234872.5 65 170933.5 66 122936.5 67 89615.0 68 65757.5 69 49166.5 70 37592.0 71 29448.0 72 23708.5 73 18233.5 74 14190.0 75 11612.5 76 8748.0 77 5551.0 78 3543.5 79 2478.5 80 1685.0 81 1237.5 82 913.5 83 692.0 84 543.0 85 421.5 86 346.0 87 301.5 88 267.5 89 228.0 90 190.0 91 161.0 92 146.5 93 124.5 94 95.0 95 86.5 96 82.0 97 61.5 98 45.5 99 35.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005993246160855895 2 0.009306009924717992 3 0.012257470050369633 4 0.012821396674873793 5 0.015672765927777943 6 0.004530942922423031 7 0.002499830577888569 8 0.005934656381686632 9 0.0071894541522283546 10-11 0.008146420545326322 12-13 0.009254743867944887 14-15 0.012112216222845834 16-17 0.020646794055168526 18-19 0.03480110820614306 20-21 0.028065724842476495 22-23 0.03843489513503675 24-25 1.415919663257197E-4 26-27 0.006481494320599757 28-29 0.009697829072912442 30-31 0.011133278662559393 32-33 0.013170494109090653 34-35 0.010986804214636233 36-37 0.014536368335974105 38-39 0.012036537758085537 40-41 0.012029214035689378 42-43 0.0023240612403807787 44-45 0.004839759883461023 46-47 0.007503153594863785 48-49 0.013982206674664823 50-51 0.01089769892548298 52-53 0.0016356313351419346 54-55 0.00959651757976559 56-57 0.015456716117091283 58-59 0.014421630018434297 60-61 0.016962961689901097 62-63 0.017899177536209947 64-65 0.01697394727349533 66-67 0.015384699513529063 68-69 0.009198595329574344 70-71 0.0017381634486881457 72-73 0.011069806401792691 74-75 0.011949873709731 76-77 0.015271181816388615 78-79 0.007019787916717362 80-81 0.0015172311564040483 82-83 0.00955501648618736 84-85 0.013363352132189478 86-87 0.012323383551935055 88-89 0.013518370922908153 90-91 0.013050873309953408 92-93 0.013598931869265892 94-95 0.011962079913724596 96-97 0.012945899955608478 98-99 0.013547665812492785 100 0.012086583194459282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.0962776E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.578539951764895 #Duplication Level Percentage of deduplicated Percentage of total 1 72.7564178018775 33.161312955301725 2 14.150580904352214 12.899256341793977 3 5.002306851972257 6.8399352811081435 4 2.3565122384408723 4.296255488264008 5 1.3902891841452265 3.168367556203491 6 0.8981425325964805 2.4561615182596794 7 0.6423752400391282 2.049496787950358 8 0.43311421367571584 1.5792570793356677 9 0.3383629682944874 1.3879881061747246 >10 1.7763283962861107 14.476906578381216 >50 0.15237254354302612 4.920570655090814 >100 0.10212674182948507 7.551254432883988 >500 6.903401788105478E-4 0.20016108980478592 >1k 3.3727593557474756E-4 0.31628327527785155 >5k 2.1383417113050027E-5 0.06262452491888967 >10k+ 2.1383416085704786E-5 4.634168329250524 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1795557 4.38338700482604 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45618 0.11136452275597727 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001750369652681742 1.2206203993596529E-5 0.0 4.882481597438611E-6 0.0 2 0.0017576933750779 2.197116718847375E-5 0.0 4.882481597438611E-6 7.323722396157917E-6 3 0.0017576933750779 2.9294889584631667E-5 0.0 7.323722396157917E-6 9.764963194877222E-6 4 0.0017625758566753387 3.6618611980789586E-5 0.0 7.323722396157917E-6 1.2206203993596529E-5 5 0.0017674583382727772 4.6383575175666806E-5 0.0 1.4647444792315834E-5 1.2206203993596529E-5 6 0.0017698995790714966 7.079598316285986E-5 0.0 1.708868559103514E-5 1.4647444792315834E-5 7 0.001774782060668935 7.567846476029847E-5 0.0 1.9529926389754444E-5 1.708868559103514E-5 8 0.001774782060668935 7.811970555901777E-5 0.0 1.9529926389754444E-5 1.708868559103514E-5 9 0.0017845470238638124 9.276715035133361E-5 0.0 1.9529926389754444E-5 1.708868559103514E-5 10-11 0.0017991944686561283 1.1229707674108805E-4 0.0 2.197116718847375E-5 1.830930599039479E-5 12-13 0.0018138419134484441 1.293857623321232E-4 0.0 2.5633028386552708E-5 2.5633028386552708E-5 14-15 0.0018297099786401196 1.47695068322518E-4 0.0 2.9294889584631667E-5 3.173613038335097E-5 16-17 0.0018467986642311546 1.5135692952059695E-4 0.0 4.1501093578228196E-5 3.417737118207028E-5 18-19 0.0018553430070266722 1.647837539135531E-4 0.0 4.5162954776307155E-5 4.1501093578228196E-5 20-21 0.0018602254886241109 2.0262298629370238E-4 0.0 5.1266056773105416E-5 4.6383575175666806E-5 22-23 0.0018895203782087425 2.2093229228409714E-4 0.0 5.4927917971184375E-5 6.1031019967982636E-5 24-25 0.00200425869574855 2.4412407987193054E-4 0.0 5.8589779169263334E-5 6.835474236414055E-5 26-27 0.0024034015663391564 2.7097772865784293E-4 0.0 6.1031019967982636E-5 7.079598316285986E-5 28-29 0.003888896592359854 2.9416951624567636E-4 0.0 6.347226076670194E-5 7.445784436093883E-5 30-31 0.007868119094272322 3.1858192423286935E-4 0.0 6.46928811660616E-5 8.056094635773708E-5 32-33 0.017194879565779427 3.332293690251852E-4 0.0 7.079598316285986E-5 8.544342795517569E-5 34-35 0.03573000033005576 3.4421495261942206E-4 0.0 7.201660356221951E-5 9.398777075069327E-5 36-37 0.06778105077644152 3.6374487900917653E-4 0.0 7.323722396157917E-5 9.764963194877223E-5 38-39 0.1180681699892605 3.857160461976503E-4 0.0 7.323722396157917E-5 1.0741459514364944E-4 40-41 0.19124192169007295 3.9792225019124683E-4 0.0 7.689908515965812E-5 1.1473831753980737E-4 42-43 0.29317226937939944 4.1745217658100125E-4 0.0 8.300218715645639E-5 1.1840017873788631E-4 44-45 0.4287661070626659 4.3698210297075566E-4 0.0 8.300218715645639E-5 1.2206203993596527E-4 46-47 0.6027081758326145 4.589532701592294E-4 0.0 8.300218715645639E-5 1.2572390113404423E-4 48-49 0.8194940206200869 4.772625761496242E-4 0.0 8.666404835453535E-5 1.293857623321232E-4 50-51 1.0830247442214365 4.967925025393786E-4 0.0 9.398777075069327E-5 1.3304762353020215E-4 52-53 1.3975651943120262 5.114399473316945E-4 0.0 1.0131149314685119E-4 1.367094847282811E-4 54-55 1.764260312826455 5.346317349195278E-4 0.0 1.0497335434493013E-4 1.367094847282811E-4 56-57 2.1834469909949465 5.602647633060807E-4 0.0 1.1595893793916701E-4 1.367094847282811E-4 58-59 2.6531649124561283 5.883390324913527E-4 0.0 1.2206203993596527E-4 1.4159196632571974E-4 60-61 3.1752901707638173 5.993246160855895E-4 0.0 1.2816514193276352E-4 1.452538275237987E-4 62-63 3.7450293896097273 6.115308200791861E-4 0.0 1.4037134592636007E-4 1.550187907186759E-4 64-65 4.362859831570009 6.371638484657387E-4 0.0 1.4891568872187766E-4 1.5868065191675486E-4 66-67 5.0226124811462975 6.615762564529318E-4 0.0 1.6112189271547415E-4 1.647837539135531E-4 68-69 5.724099362797092 6.762237012452476E-4 0.0 1.6600437431291278E-4 1.7088685591035139E-4 70-71 6.463653732842715 6.95753627635002E-4 0.0 1.6844561511163207E-4 1.733280967090707E-4 72-73 7.23490639403931 7.201660356221951E-4 0.0 1.6844561511163207E-4 1.8431368030330758E-4 74-75 8.036391381287245 7.372547212132303E-4 0.0 1.7454871710843035E-4 1.8553430070266722E-4 76-77 8.867012577467893 7.494609252068268E-4 0.0 1.8431368030330758E-4 1.9041678230010583E-4 78-79 9.719404759091523 7.628877495997829E-4 0.0 1.916374026994655E-4 1.965198842969041E-4 80-81 10.595003375747776 7.860795371876164E-4 0.0 1.965198842969041E-4 2.014023658943427E-4 82-83 11.488462110087461 8.068300839767304E-4 0.0 2.0384360669306202E-4 2.03843606693062E-4 84-85 12.399636928903451 8.15374426772248E-4 0.0 2.0872608829050062E-4 2.0872608829050062E-4 86-87 13.323575775235547 8.226981491684059E-4 0.0 2.1360856988793925E-4 2.1238794948857958E-4 88 14.023373318253626 8.397868347594411E-4 0.0 2.197116718847375E-4 2.197116718847375E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 220825 0.0 85.22888 8 TGGTATC 223280 0.0 84.039024 7 AGTGGTA 223870 0.0 83.92906 5 GTGGTAT 223920 0.0 83.880684 6 GTATCAA 223960 0.0 83.878456 9 AAGCAGT 236290 0.0 80.40686 1 CAGTGGT 238660 0.0 78.93135 4 GCAGTGG 241900 0.0 78.478195 3 AGCAGTG 254120 0.0 74.79639 2 CAACGCA 215725 0.0 45.15186 12-13 ATCAACG 218710 0.0 44.600574 10-11 ACGCAGA 220475 0.0 44.1327 14-15 TCAACGC 215885 0.0 43.790035 12-13 AGAGTAC 221880 0.0 43.22493 18-19 AACGCAG 218445 0.0 43.163433 14-15 GAGTACT 216605 0.0 42.41508 20-21 CGCAGAG 222770 0.0 42.223015 16-17 TATCAAC 225535 0.0 41.67626 10-11 AGTACTT 227615 0.0 41.574783 20-21 GTACTTT 226300 0.0 40.654106 22-23 >>END_MODULE