FastQCFastQC Report
Thu 26 May 2016
SRR1660575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40962776
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT16899234.125508974294125No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA481120.11745297730798322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2078550.084.577198
AGTGGTA2101150.083.643485
GTGGTAT2107350.083.4107746
TGGTATC2107100.083.364917
GTATCAA2107800.083.176089
AAGCAGT2227300.079.28781
CAGTGGT2248550.078.340124
GCAGTGG2267450.077.952433
AGCAGTG2394450.073.9592062
CAACGCA2015050.044.89075512-13
ATCAACG2041000.044.36792410-11
ACGCAGA2060600.043.90531514-15
TCAACGC2013600.043.59848412-13
AACGCAG2037000.043.00189614-15
AGAGTAC2098800.042.9423418-19
GAGTACT2043550.042.23670220-21
CGCAGAG2090900.041.90341616-17
TATCAAC2123650.041.3321210-11
AGTACTT2157050.041.20834720-21
GTACTTT2145400.040.2822722-23
Produced by FastQC (version 0.11.5)