FastQCFastQC Report
Thu 26 May 2016
SRR1660574_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660574_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40926027
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19849744.850150736596054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538340.1315397656361806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2425100.085.999018
GTGGTAT2459150.084.683946
AGTGGTA2461250.084.6411065
GTATCAA2461800.084.587039
TGGTATC2466000.084.3972557
AAGCAGT2607000.080.768611
CAGTGGT2609400.079.987984
GCAGTGG2668550.078.8154753
AGCAGTG2738650.076.9025652
CAACGCA2379400.045.18055712-13
ATCAACG2412250.044.5893710-11
ACGCAGA2425050.044.22978614-15
TCAACGC2380900.044.0878312-13
AACGCAG2413250.043.42436214-15
AGAGTAC2438800.043.36478818-19
GAGTACT2389000.042.6532620-21
CGCAGAG2457650.042.5197916-17
TATCAAC2482400.042.0312910-11
AGTACTT2503050.041.70427320-21
GTACTTT2497450.040.8691922-23
Produced by FastQC (version 0.11.5)