FastQCFastQC Report
Thu 26 May 2016
SRR1660574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40926027
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18467514.512412113689902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA553640.13527821794184908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2248800.085.33648
GTATCAA2276400.084.058139
GTGGTAT2285600.084.006426
AGTGGTA2288850.083.891255
TGGTATC2293100.083.657887
AAGCAGT2418550.079.832981
CAGTGGT2444150.078.716594
GCAGTGG2484050.077.7435763
AGCAGTG2547300.075.9331052
CAACGCA2189100.045.077612-13
ATCAACG2218000.044.5228210-11
ACGCAGA2236850.044.11847714-15
TCAACGC2190350.043.82021712-13
AGAGTAC2271150.043.19113518-19
AACGCAG2220700.043.15781414-15
GAGTACT2216100.042.36359420-21
CGCAGAG2270550.042.17503416-17
TATCAAC2306200.041.58710510-11
AGTACTT2339950.041.22710820-21
GTACTTT2329350.040.33970322-23
Produced by FastQC (version 0.11.5)