FastQCFastQC Report
Thu 26 May 2016
SRR1660573_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660573_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41519436
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22102555.323422505064857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA557470.13426723811951588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2670600.086.263958
AGTGGTA2701350.085.131525
GTATCAA2704500.085.039659
TGGTATC2705000.084.9347847
GTGGTAT2712700.084.792446
AAGCAGT2819600.082.492481
CAGTGGT2838200.081.239944
GCAGTGG2865300.081.065223
AGCAGTG2945350.078.9702152
CAACGCA2631050.045.129612-13
ATCAACG2655150.044.77121410-11
ACGCAGA2680400.044.25702314-15
TCAACGC2630600.044.00428412-13
AGAGTAC2694700.043.50994518-19
AACGCAG2655700.043.5048214-15
GAGTACT2617450.043.02551720-21
CGCAGAG2710650.042.5326216-17
AGTACTT2721200.042.45622620-21
TATCAAC2719100.042.3378410-11
GTACTTT2719950.041.43551622-23
Produced by FastQC (version 0.11.5)