##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660573_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41519436 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.995011420675368 34.0 31.0 34.0 30.0 34.0 2 32.14453163573802 34.0 31.0 34.0 30.0 34.0 3 32.16601687460302 34.0 31.0 34.0 30.0 34.0 4 35.59070489300481 37.0 35.0 37.0 33.0 37.0 5 35.582235558305754 37.0 35.0 37.0 33.0 37.0 6 35.60341407335109 37.0 35.0 37.0 33.0 37.0 7 35.542406669493296 37.0 35.0 37.0 33.0 37.0 8 35.5707761059182 37.0 35.0 37.0 33.0 37.0 9 37.249060560456556 39.0 37.0 39.0 34.0 39.0 10-11 37.15117400679527 39.0 37.0 39.0 34.0 39.0 12-13 37.19995425756747 39.0 37.0 39.0 34.0 39.0 14-15 38.599997360272425 40.0 38.0 41.0 34.0 41.0 16-17 38.5591407961322 40.0 38.0 41.0 34.0 41.0 18-19 38.519305356171024 40.0 38.0 41.0 34.0 41.0 20-21 38.449419339414916 40.0 38.0 41.0 34.0 41.0 22-23 38.285383573129465 40.0 38.0 41.0 33.5 41.0 24-25 38.292466436682815 40.0 38.0 41.0 34.0 41.0 26-27 38.2857453795856 40.0 38.0 41.0 34.0 41.0 28-29 38.22345032577032 40.0 38.0 41.0 34.0 41.0 30-31 38.13302076165004 40.0 38.0 41.0 33.5 41.0 32-33 38.02448696557438 40.0 38.0 41.0 33.0 41.0 34-35 37.92546768939732 40.0 38.0 41.0 33.0 41.0 36-37 37.74886930063308 40.0 38.0 41.0 33.0 41.0 38-39 37.34916890489553 40.0 36.0 41.0 32.5 41.0 40-41 37.15060654966508 40.0 36.0 41.0 32.0 41.0 42-43 37.042714296504414 40.0 36.0 41.0 32.0 41.0 44-45 36.9168411752992 40.0 35.0 41.0 31.0 41.0 46-47 36.758670240607316 39.5 35.0 41.0 31.0 41.0 48-49 36.558321264286924 39.0 35.0 41.0 31.0 41.0 50-51 35.45383665616267 38.0 34.0 40.0 29.0 40.5 52-53 35.301837553862725 38.0 35.0 39.5 28.0 40.5 54-55 35.62200955716258 39.0 35.0 40.5 28.0 41.0 56-57 35.447266251400904 39.0 35.0 41.0 27.0 41.0 58-59 35.25234461277364 39.0 35.0 41.0 26.0 41.0 60-61 35.02633068040712 39.0 35.0 41.0 26.0 41.0 62-63 34.75489152839167 38.5 35.0 41.0 26.0 41.0 64-65 34.434653941349296 37.5 35.0 40.5 26.0 41.0 66-67 34.09601860921232 37.0 34.0 40.0 25.0 41.0 68-69 33.747064784309686 36.5 34.0 39.5 24.0 41.0 70-71 33.37990324579553 36.0 34.0 39.0 23.5 41.0 72-73 33.000344320669484 35.5 34.0 39.0 22.5 41.0 74-75 32.607160186376326 35.0 33.5 37.5 20.5 40.0 76-77 32.2289211972918 35.0 33.0 37.0 20.0 39.5 78-79 31.866699068359214 35.0 33.0 37.0 18.5 39.0 80-81 31.518560054621165 35.0 33.0 36.0 16.0 39.0 82-83 31.222504106269653 35.0 33.0 36.0 11.0 37.0 84-85 30.95847956605191 35.0 33.0 35.0 7.0 37.0 86-87 30.714592353807504 35.0 32.5 35.0 2.0 36.5 88-89 30.493837488543917 35.0 32.0 35.0 2.0 36.0 90-91 30.29393851833633 35.0 32.0 35.0 2.0 36.0 92-93 30.100636025017295 35.0 32.0 35.0 2.0 36.0 94-95 29.915316166626155 35.0 32.0 35.0 2.0 35.5 96-97 29.71175754892239 35.0 32.0 35.0 2.0 35.0 98-99 29.52848350348497 35.0 31.5 35.0 2.0 35.0 100 29.393217070675046 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3452219598555075 1101 2 0.3101216838703209 1101 3 0.3133304159389354 1101 4 0.24704347409608118 1101 5 0.2579500340752716 1101 6 0.26220979704885394 1101 7 0.22201363859960566 1101 8 0.23639998145127095 1101 9 0.34793880034081326 1101 10-11 0.37095776722465956 1101 12-13 0.3893253588736414 1101 14-15 0.4430278815791837 1101 16-17 0.4309963162917896 1101 18-19 0.4522018364938205 1101 20-21 0.45427429388238494 1101 22-23 0.4449676073905664 1101 24-25 0.4219479826391037 1101 26-27 0.36144220525676474 1101 28-29 0.44729772255299594 1101 30-31 0.39273188015298643 1101 32-33 0.3602279870015792 1101 34-35 0.4135478519475342 1101 36-37 0.4052903929251812 1101 38-39 0.4173081331770945 1101 40-41 0.5313722255872477 1101 42-43 0.4597854432432129 1101 44-45 0.41382448832207785 1101 46-47 0.4044479664001912 1101 48-49 0.4597742670983038 1101 50-51 0.3955195949636163 1101 52-53 0.4260987357785453 1101 54-55 0.48499810464620197 1101 56-57 0.49493003963380033 1101 58-59 0.5075735579477154 1101 60-61 0.4808897904164624 1101 62-63 0.4622142987157858 1101 64-65 0.43994794419031535 1101 66-67 0.4417884138759334 1101 68-69 0.41882057631118386 1101 70-71 0.42513551950325024 1101 72-73 0.3864905153487683 1101 74-75 0.455374013210772 1101 76-77 0.40086863434114406 1101 78-79 0.44486075234854283 1101 80-81 0.4032488935606189 1101 82-83 0.37810445117365177 1101 84-85 0.3613113171918272 1101 86-87 0.37696524979245183 1101 88-89 0.37120894818657746 1101 90-91 0.35842783498901554 1101 92-93 0.32111121152849265 1101 94-95 0.2568199755850067 1101 96-97 0.3069487433652789 1101 98-99 0.3734866159297141 1101 100 0.3355698860059242 1102 1 0.33943183242934083 1102 2 0.3520095103886902 1102 3 0.359133035392702 1102 4 0.3104672588816584 1102 5 0.3093203215206515 1102 6 0.31263423109145805 1102 7 0.32721504041954574 1102 8 0.3216460213915582 1102 9 0.3574523176542712 1102 10-11 0.382898042963582 1102 12-13 0.4012974440283372 1102 14-15 0.4451848611795697 1102 16-17 0.4575723058403227 1102 18-19 0.4723731356025169 1102 20-21 0.46309643919196475 1102 22-23 0.4980780666373974 1102 24-25 0.48941311786762043 1102 26-27 0.48266411750621785 1102 28-29 0.4887287456992695 1102 30-31 0.49766939985320846 1102 32-33 0.46936210264006206 1102 34-35 0.4834132134637841 1102 36-37 0.47138458362951496 1102 38-39 0.460570960861439 1102 40-41 0.5576320096242711 1102 42-43 0.5149061203205321 1102 44-45 0.4771721564186322 1102 46-47 0.4771278721481238 1102 48-49 0.5074213772049845 1102 50-51 0.4063245271890352 1102 52-53 0.3927458406868922 1102 54-55 0.4786601787571101 1102 56-57 0.49237769535152154 1102 58-59 0.47340420903920943 1102 60-61 0.44188899934702164 1102 62-63 0.436228010067893 1102 64-65 0.4206650921447377 1102 66-67 0.4215140894258127 1102 68-69 0.4589224298418344 1102 70-71 0.40850495209031834 1102 72-73 0.42865746675768435 1102 74-75 0.3887294491690767 1102 76-77 0.356240600944723 1102 78-79 0.4094381258192179 1102 80-81 0.38101772526915667 1102 82-83 0.33770036568354556 1102 84-85 0.33114321384239176 1102 86-87 0.32524614580794875 1102 88-89 0.30641560347910257 1102 90-91 0.24368986344206078 1102 92-93 0.24258966872008614 1102 94-95 0.2646640451244906 1102 96-97 0.2834365568246646 1102 98-99 0.3290477872216364 1102 100 0.31969913731794364 1103 1 0.3621817517326491 1103 2 0.3304873558959045 1103 3 0.36045687260500614 1103 4 0.33698714546311237 1103 5 0.3318495947093254 1103 6 0.3080047023436592 1103 7 0.33376456099573204 1103 8 0.35622888413306697 1103 9 0.3846546544880809 1103 10-11 0.40022152186115534 1103 12-13 0.3884933429782862 1103 14-15 0.4666464602552338 1103 16-17 0.46939669186733823 1103 18-19 0.5197623878765327 1103 20-21 0.5183458348308392 1103 22-23 0.47040145689053503 1103 24-25 0.4752318306599861 1103 26-27 0.46308303475886703 1103 28-29 0.5078654966038485 1103 30-31 0.48516361844690437 1103 32-33 0.4589723714229592 1103 34-35 0.4876971758769386 1103 36-37 0.5053541352639783 1103 38-39 0.5337369262765534 1103 40-41 0.5843039591216623 1103 42-43 0.5301539379482136 1103 44-45 0.5247102708034674 1103 46-47 0.47511835756133536 1103 48-49 0.5400797347525881 1103 50-51 0.4445022373110632 1103 52-53 0.5052597191761734 1103 54-55 0.642828254611409 1103 56-57 0.64349888267175 1103 58-59 0.6378777199961547 1103 60-61 0.6069950613311406 1103 62-63 0.6094722358812348 1103 64-65 0.5903886834211605 1103 66-67 0.5524281641048319 1103 68-69 0.5216669761786861 1103 70-71 0.5332289810623152 1103 72-73 0.5276247593847216 1103 74-75 0.5532555919330449 1103 76-77 0.4995277053094256 1103 78-79 0.5408903458669521 1103 80-81 0.5031863165155848 1103 82-83 0.5032039887186279 1103 84-85 0.5075128257480479 1103 86-87 0.48452030794571854 1103 88-89 0.4674200974051246 1103 90-91 0.4781479621110911 1103 92-93 0.44764361984218937 1103 94-95 0.47077969749713233 1103 96-97 0.5189798578252827 1103 98-99 0.5379723725332575 1103 100 0.48612708686327366 1104 1 0.27393587066773506 1104 2 0.28682584290490354 1104 3 0.25655414267007615 1104 4 0.1954470954395191 1104 5 0.21968959043685032 1104 6 0.24511063307612346 1104 7 0.27481612445537706 1104 8 0.2827942574852713 1104 9 0.31705623174426023 1104 10-11 0.3403044791274894 1104 12-13 0.3256989648462465 1104 14-15 0.3834013357748347 1104 16-17 0.343837308884261 1104 18-19 0.35398172617662027 1104 20-21 0.3336852742462142 1104 22-23 0.3641278581295566 1104 24-25 0.3720604064118973 1104 26-27 0.33066169729546857 1104 28-29 0.36548181034609684 1104 30-31 0.3798078902120636 1104 32-33 0.350479398757912 1104 34-35 0.4050153063121016 1104 36-37 0.3776741794129066 1104 38-39 0.40848569571409854 1104 40-41 0.4892653733116248 1104 42-43 0.4131637201408793 1104 44-45 0.38614671325409233 1104 46-47 0.3801207163248108 1104 48-49 0.3810422882698177 1104 50-51 -0.008860647323146509 1104 52-53 0.24217837298980527 1104 54-55 0.3461527759604408 1104 56-57 0.4022719696255592 1104 58-59 0.42541739617798413 1104 60-61 0.38846532755987084 1104 62-63 0.4139853960012161 1104 64-65 0.37577289483757426 1104 66-67 0.40368400538230276 1104 68-69 0.34888034191163086 1104 70-71 0.2969983741035165 1104 72-73 0.36778230698278946 1104 74-75 0.34681910497745605 1104 76-77 0.33834778951283084 1104 78-79 0.3293665846628251 1104 80-81 0.3394833248914182 1104 82-83 0.33517208755526795 1104 84-85 0.31030560170112054 1104 86-87 0.31907361154972946 1104 88-89 0.3554822619489002 1104 90-91 0.30675201918426254 1104 92-93 0.36853176104756 1104 94-95 0.40166027285740213 1104 96-97 0.3437614719315789 1104 98-99 0.3813031245782632 1104 100 0.3432426306695149 1105 1 0.1941354407344349 1105 2 0.2095940812139716 1105 3 0.18544252902744773 1105 4 0.19486011274492654 1105 5 0.18800918016401624 1105 6 0.1563777254540284 1105 7 0.19835939580406858 1105 8 0.20064842602729982 1105 9 0.24105068942579777 1105 10-11 0.25445101167333206 1105 12-13 0.23811015783613954 1105 14-15 0.26214928913725544 1105 16-17 0.31108274779674616 1105 18-19 0.3125293331043082 1105 20-21 0.28311491990108806 1105 22-23 0.33203387703642306 1105 24-25 0.2871103090522311 1105 26-27 0.28722293258563525 1105 28-29 0.2643086435637869 1105 30-31 0.2958300967276841 1105 32-33 0.310877038599088 1105 34-35 0.28132806101326224 1105 36-37 0.3026649044868748 1105 38-39 0.3051826082056408 1105 40-41 0.3798940402316191 1105 42-43 0.2763068891112326 1105 44-45 0.3043867167937222 1105 46-47 0.2734024376140596 1105 48-49 0.27371916807623364 1105 50-51 0.20246056676908353 1105 52-53 0.2578370109023851 1105 54-55 0.3423601550481763 1105 56-57 0.31263408772484524 1105 58-59 0.30119168504610627 1105 60-61 0.2709341330988977 1105 62-63 0.28942765260800485 1105 64-65 0.23517727517307918 1105 66-67 0.21836208515695432 1105 68-69 0.23879297179644965 1105 70-71 0.24940538220749175 1105 72-73 0.2413329679345395 1105 74-75 0.27931106613289813 1105 76-77 0.25134278006364497 1105 78-79 0.22673465454085928 1105 80-81 0.21864952288272477 1105 82-83 0.231140167045794 1105 84-85 0.23861601076841055 1105 86-87 0.25139213593665133 1105 88-89 0.2160617587827538 1105 90-91 0.21283375377699443 1105 92-93 0.20850865920267836 1105 94-95 0.19799348961935337 1105 96-97 0.1761242662245195 1105 98-99 0.20282595803015724 1105 100 0.21696207748389185 1106 1 0.15093827937075588 1106 2 0.16158521184556207 1106 3 0.14046118494381687 1106 4 0.12237612301196066 1106 5 0.1361458432464886 1106 6 0.13906504088960503 1106 7 0.14803384160286726 1106 8 0.17013616464623738 1106 9 0.17919167036284023 1106 10-11 0.22211601019184712 1106 12-13 0.2076449578725672 1106 14-15 0.1951874054212368 1106 16-17 0.159408253619425 1106 18-19 0.20785052889714706 1106 20-21 0.23992069728234355 1106 22-23 0.22723045804171704 1106 24-25 0.20009093704997127 1106 26-27 0.20541783897493815 1106 28-29 0.22739374123028 1106 30-31 0.16086001926730376 1106 32-33 0.2281152472078034 1106 34-35 0.1497093172851791 1106 36-37 0.2363309660151245 1106 38-39 0.24962642194109463 1106 40-41 0.32384214586006976 1106 42-43 0.23584200780644693 1106 44-45 0.24709834373889095 1106 46-47 0.2587363638737159 1106 48-49 0.2128175197108959 1106 50-51 0.1809231027881495 1106 52-53 0.2161460172843661 1106 54-55 0.20724524464375094 1106 56-57 0.2155785334200715 1106 58-59 0.20647993400329057 1106 60-61 0.223305444622234 1106 62-63 0.23517575578382832 1106 64-65 0.19919025585266326 1106 66-67 0.1876386363591962 1106 68-69 0.19521199872581718 1106 70-71 0.15887806875750954 1106 72-73 0.10457513503713756 1106 74-75 0.14657971983259444 1106 76-77 0.13006236988262998 1106 78-79 0.1708681992516965 1106 80-81 0.21883065248688283 1106 82-83 0.21276175937090258 1106 84-85 0.1931900887811473 1106 86-87 0.2160981413660359 1106 88-89 0.251514432172101 1106 90-91 0.25909575859151346 1106 92-93 0.2362345013480187 1106 94-95 0.27194685819968356 1106 96-97 0.23036313437876643 1106 98-99 0.22285086155352118 1106 100 0.21468464146341404 1107 1 0.15922996601230466 1107 2 0.10012922634374632 1107 3 0.15210365418645466 1107 4 0.1825274441232949 1107 5 0.16287832020341142 1107 6 0.17047867197960187 1107 7 0.10501850423824521 1107 8 0.12605454570641683 1107 9 0.17044504545459915 1107 10-11 0.17501450940265073 1107 12-13 0.17612925880964525 1107 14-15 0.2329342379132484 1107 16-17 0.2283450796497064 1107 18-19 0.20121471026208582 1107 20-21 0.24021575286493402 1107 22-23 0.2681175244105489 1107 24-25 0.2235244086648862 1107 26-27 0.23279818668405028 1107 28-29 0.2274773630896405 1107 30-31 0.20716210994945072 1107 32-33 0.20039463546996217 1107 34-35 0.1626677290824503 1107 36-37 0.1691570645510012 1107 38-39 0.14747940939029291 1107 40-41 0.24070923607563088 1107 42-43 0.19705718418607887 1107 44-45 0.1774927811026572 1107 46-47 0.15038189026063264 1107 48-49 0.17795377015738012 1107 50-51 0.18880384567250275 1107 52-53 0.22285874287172902 1107 54-55 0.17845812149605678 1107 56-57 0.182626709967181 1107 58-59 0.22666070518121728 1107 60-61 0.22156085080047916 1107 62-63 0.200155454190714 1107 64-65 0.1933281675946219 1107 66-67 0.20563863393253 1107 68-69 0.19346478274068346 1107 70-71 0.2050948950005349 1107 72-73 0.2216471828197939 1107 74-75 0.23141695688953945 1107 76-77 0.2103968999855823 1107 78-79 0.26699439184097074 1107 80-81 0.21289385260412885 1107 82-83 0.188797239578264 1107 84-85 0.23666818933354605 1107 86-87 0.18272068883614878 1107 88-89 0.20595991409291514 1107 90-91 0.21866998211911692 1107 92-93 0.19101019421536236 1107 94-95 0.24442966338368777 1107 96-97 0.18688835091575484 1107 98-99 0.2261423772378457 1107 100 0.24812888069024552 1108 1 0.0993383266527843 1108 2 0.1358683068076516 1108 3 0.126481073793137 1108 4 0.10478458034238258 1108 5 0.08140331441568804 1108 6 0.13197317422955734 1108 7 0.1170012838930532 1108 8 0.08377713466772008 1108 9 0.16612339508802165 1108 10-11 0.16744590072853072 1108 12-13 0.17588433916434099 1108 14-15 0.21183229960658423 1108 16-17 0.20038420969066095 1108 18-19 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68-69 -1.0000156806539806 2316 70-71 -0.9375735610322877 2316 72-73 -0.9985514545109169 2316 74-75 -0.9798578976390289 2316 76-77 -0.9270103261839608 2316 78-79 -0.8850253685933076 2316 80-81 -0.9304804763775643 2316 82-83 -0.9242023005652023 2316 84-85 -0.927532089798536 2316 86-87 -0.9565705244390976 2316 88-89 -0.9703112417540574 2316 90-91 -0.9449799089382189 2316 92-93 -0.9546115510780915 2316 94-95 -0.9865562660289413 2316 96-97 -0.9906492383829111 2316 98-99 -0.9706701038285992 2316 100 -1.0180489973276075 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 270304.0 3 40021.0 4 34221.0 5 35146.0 6 36297.0 7 38296.0 8 44607.0 9 55775.0 10 69094.0 11 82115.0 12 89994.0 13 94302.0 14 92463.0 15 88033.0 16 101875.0 17 131940.0 18 184033.0 19 281157.0 20 541061.0 21 654807.0 22 296620.0 23 243555.0 24 271411.0 25 292780.0 26 308907.0 27 352409.0 28 419191.0 29 507571.0 30 618751.0 31 775072.0 32 990180.0 33 1293862.0 34 1767273.0 35 2684260.0 36 4591198.0 37 8668081.0 38 1.0980205E7 39 3480142.0 40 12427.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 16.49122189217427 34.216183433705 27.13247144901097 22.16012322510976 2 21.67178867480667 32.68450201078543 26.555618177236333 19.08809113717156 3 26.049784992459717 25.56911514806516 26.192634365199623 22.1884654942755 4 20.32410281155664 26.961578717604734 25.370454710404182 27.343863760434452 5 21.63769782298928 31.819700621972437 25.65309466329445 20.889506891743835 6 27.479171235012135 26.15284405759256 25.747784360477237 20.620200346918065 7 21.306862760895427 26.074540865693617 31.584487333533772 21.03410903987718 8 26.81313724255643 26.17636136285694 25.82660311224121 21.183898282345417 9 26.584733450244524 25.607040627352834 26.503820874317324 21.304405048085318 10-11 20.908878942798847 29.233763532572414 28.851852061498644 21.00550546313009 12-13 20.942151254067014 26.23558027949721 28.709500645474474 24.112767820961306 14-15 20.940988164179316 32.214072851399024 25.770741351007686 21.074197633413977 16-17 24.03609746325196 26.11773364723731 25.733284150932924 24.11288473857781 18-19 21.07023977446597 29.10592917077912 25.72841687076836 24.09541418398655 20-21 24.036624605288356 29.18717655050795 25.691280968582486 21.084917875621212 22-23 24.083567272924235 26.23025857617336 28.615903895739912 21.070270255162495 24-25 21.12509387182564 29.119589454370466 25.52618680722111 24.229129866582785 26-27 21.119033196854932 26.209951541432574 31.538269511953548 21.13274574975894 28-29 21.188072571158113 26.243585861961137 31.496602882613363 21.071738684267387 30-31 21.172090890534555 26.170384511862725 31.50763662266326 21.149887974939453 32-33 21.206571096611242 26.228950948600744 31.417923939878182 21.14655401490983 34-35 21.1860962743703 26.385135467562172 31.324749740764773 21.104018517302755 36-37 21.142275809876505 26.356915958267894 31.349850900162284 21.150957331693316 38-39 21.175481138223024 26.286730661657003 31.37356820176879 21.16421999835118 40-41 21.302417149284157 26.356689448464426 31.276267853625367 21.064625548626047 42-43 21.244675285988073 26.388808034562583 31.292569200658782 21.07394747879056 44-45 21.3346375103404 26.432378466743216 31.206853526525723 21.02613049639066 46-47 21.275201366327607 26.554058313213304 31.172371513025105 20.998368807433984 48-49 21.416365606409915 26.575561658032658 30.98733086279927 21.02074187275816 50-51 21.47420710553399 26.607116855243877 30.925888522955354 20.992787516266777 52-53 21.542668196431553 26.82397829985374 30.72259786130372 20.91075564241099 54-55 21.896077692125075 27.038833440731086 29.995553945017488 21.06953492212635 56-57 22.35485358880471 27.55803276533956 28.977175687604856 21.109937958250875 58-59 22.511892408481124 28.01980263846004 28.413909773548617 21.054395179510223 60-61 22.611813524723445 28.2433883642534 28.098395612686517 21.04640249833664 62-63 22.729716868928957 28.360830581211655 27.95140983536027 20.958042714499122 64-65 22.85625210847984 28.4180776064635 27.93108613741404 20.794584147642617 66-67 22.995134980784616 28.227329911858657 28.077514530716503 20.70002057664023 68-69 23.121560370701193 28.037240657272662 28.286860996206308 20.554337975819838 70-71 23.28071467078176 27.95014703917434 28.409796574182028 20.359341715861873 72-73 23.46621496820549 27.874862494542903 28.37491288631241 20.2840096509392 74-75 23.715543252687173 27.9349969762327 28.204440585224834 20.145019185855293 76-77 23.994513472815708 28.086041461314142 27.872287747805423 20.04715731806472 78-79 24.289318069724143 28.21681741543578 27.489103995701175 20.0047605191389 80-81 24.553252183206265 28.360115913246503 27.164462651560374 19.922169251986862 82-83 24.727047842874676 28.526557864096517 26.89428927719152 19.85210501583729 84-85 24.941151761694197 28.538045502591693 26.704009422173787 19.816793313540327 86-87 25.148554425079794 28.481946734032 26.608108253124783 19.761390587763422 88-89 25.357563461133196 28.462457033470596 26.51192386869304 19.668055636703162 90-91 25.54473651710441 28.380014424510286 26.39514717676018 19.68010188162512 92-93 25.70045806316073 28.348772751356165 26.341488020018588 19.609281165464516 94-95 25.836749965177784 28.306395020673225 26.308582090608333 19.548272923540654 96-97 25.974109860392996 28.230453764318085 26.226342197998914 19.569094177290005 98-99 26.08701611419559 28.22356026255085 26.192212300168404 19.49721132308515 100 26.171379464973082 28.265290234210372 26.09257321157788 19.470757089238663 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 77236.0 1 50800.0 2 19879.5 3 14450.5 4 13351.0 5 13083.5 6 13237.0 7 13516.5 8 13353.5 9 13489.0 10 14274.5 11 18179.5 12 223391.5 13 266929.5 14 105226.5 15 107377.5 16 118768.0 17 129206.0 18 138194.0 19 147743.0 20 159716.0 21 176788.0 22 201454.0 23 230323.5 24 260231.5 25 294824.0 26 336438.5 27 386498.0 28 443554.5 29 498690.5 30 552324.0 31 609111.5 32 669369.0 33 729874.5 34 790159.0 35 859510.0 36 933923.0 37 998187.0 38 1060913.5 39 1117834.0 40 1156779.0 41 1200332.5 42 1248870.5 43 1287005.0 44 1324844.5 45 1367332.0 46 1406162.0 47 1435889.5 48 1461591.0 49 1481814.5 50 1484679.0 51 1472175.5 52 1448799.0 53 1407759.5 54 1345131.0 55 1266975.5 56 1176000.5 57 1069281.0 58 948669.5 59 818738.0 60 678935.5 61 545186.0 62 430003.0 63 329522.5 64 246752.5 65 180676.5 66 130432.5 67 95698.5 68 69889.0 69 51239.5 70 38962.5 71 30149.0 72 23799.0 73 18724.0 74 14820.5 75 11889.5 76 9009.5 77 6138.0 78 4201.0 79 2971.0 80 2085.5 81 1543.5 82 1124.0 83 794.5 84 620.0 85 509.0 86 406.0 87 325.0 88 247.5 89 206.5 90 187.0 91 159.5 92 134.5 93 108.5 94 92.5 95 78.5 96 59.0 97 45.5 98 40.5 99 29.0 100 14.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00619709766770435 2 0.009195693313367743 3 0.012230416617412624 4 0.012974646380071252 5 0.015662544163653862 6 0.004460561554834223 7 0.0024783573649699866 8 0.005958655122386537 9 0.007278518908590184 10-11 0.008191344410362414 12-13 0.009276378417086398 14-15 0.012331574060880788 16-17 0.020838433354441518 18-19 0.03466087545119832 20-21 0.027945948013359333 22-23 0.038160441293085004 24-25 1.348765912908836E-4 26-27 0.006580050846548108 28-29 0.009676191169841517 30-31 0.011122501760380368 32-33 0.013057739994348672 34-35 0.010921391128723425 36-37 0.01439566760974306 38-39 0.012014854922403088 40-41 0.01188238684167097 42-43 0.002430187153794671 44-45 0.0049747785591307166 46-47 0.007671106129669006 48-49 0.014149999532748953 50-51 0.011105642186469008 52-53 0.0015354254812131839 54-55 0.0095858720238878 56-57 0.015467454808393833 58-59 0.014398076120301827 60-61 0.016898110080300704 62-63 0.017756744094500704 64-65 0.016870412208874898 66-67 0.015274773963692572 68-69 0.009124642251884155 70-71 0.0017786850476485276 72-73 0.011368169837374476 74-75 0.012037735772711364 76-77 0.015590288846890888 78-79 0.0071183529564322594 80-81 0.0015089318650667604 82-83 0.00954854011022693 84-85 0.013123974034714728 86-87 0.012296650657778685 88-89 0.013443101683751195 90-91 0.012945744253366063 92-93 0.013534625084984295 94-95 0.012014854922403088 96-97 0.0128373612782216 98-99 0.013423833599281069 100 0.011955846413713327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.1519436E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.49786852665852 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48518020412975 32.69933887381175 2 13.267081005096207 11.807136525945994 3 4.74239289400473 6.330791264975464 4 2.3231756439703024 4.135054574789031 5 1.4272680116648508 3.175519216768393 6 0.9145915648149566 2.4418425124036096 7 0.6366458310344614 1.9830567741191705 8 0.48097174708816054 1.7121774053572978 9 0.35392001380202825 1.4173817618804243 >10 2.080446886948215 16.948153096214714 >50 0.2222579019717931 6.8532526390589625 >100 0.06487883792919094 4.113483601563244 >500 7.584357498511798E-4 0.22733627593380465 >1k 3.716979666629335E-4 0.37386791813377923 >5k 3.235845977630015E-5 0.09516182248210804 >10k+ 2.6965369000833768E-5 5.686445736562187 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2210255 5.323422505064857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55747 0.13426723811951588 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.10296864340835458 1.2042552793828894E-5 0.0 0.0 0.0 2 0.1030095880878536 2.167659502889201E-5 2.408510558765779E-6 0.0 4.817021117531558E-6 3 0.10304571574623508 3.131063726395513E-5 2.408510558765779E-6 0.0 1.2042552793828894E-5 4 0.10308184340461657 4.817021117531558E-5 2.408510558765779E-6 0.0 1.6859573911360454E-5 5 0.1031011114890867 6.98468062042076E-5 2.408510558765779E-6 2.408510558765779E-6 1.6859573911360454E-5 6 0.10313242212635065 7.707233788050493E-5 2.408510558765779E-6 4.817021117531558E-6 1.9268084470126232E-5 7 0.10314205616858572 9.15234012330996E-5 2.408510558765779E-6 9.634042235063116E-6 2.408510558765779E-5 8 0.10316373276361461 1.0115744346816271E-4 2.408510558765779E-6 1.2042552793828894E-5 2.408510558765779E-5 9 0.10320226893255487 1.1079148570322584E-4 2.408510558765779E-6 1.2042552793828894E-5 2.649361614642357E-5 10-11 0.10323357956981882 1.4330637824656384E-4 2.408510558765779E-6 1.3246808073211784E-5 2.769787142580646E-5 12-13 0.10331185616297871 1.6739148383422163E-4 2.408510558765779E-6 1.6859573911360454E-5 3.131063726395513E-5 14-15 0.10337086467166848 1.830468024661992E-4 2.408510558765779E-6 1.6859573911360454E-5 4.937446645469847E-5 16-17 0.10343348594619639 1.9870212109817677E-4 2.408510558765779E-6 1.8063829190743343E-5 6.98468062042076E-5 18-19 0.10351898807103256 2.2639999252398323E-4 2.408510558765779E-6 2.167659502889201E-5 8.188935899803649E-5 20-21 0.10359124338779555 2.637319061848528E-4 2.408510558765779E-6 2.28808503082749E-5 9.15234012330996E-5 22-23 0.10366711147039666 2.962467987281908E-4 2.408510558765779E-6 2.8902126705189352E-5 1.0477020930631139E-4 24-25 0.10391037103683201 3.2274041487461433E-4 2.408510558765779E-6 3.251489254333802E-5 1.1319999626199162E-4 26-27 0.10474732845600311 3.4080424406535777E-4 2.408510558765779E-6 3.7331913660869576E-5 1.1681276210014028E-4 28-29 0.10794462622276468 3.660936049323984E-4 2.408510558765779E-6 4.094467949901824E-5 1.2403829377643762E-4 30-31 0.1162419450977128 3.913829657994391E-4 2.408510558765779E-6 4.214893477840113E-5 1.3005957017335208E-4 32-33 0.13527038276724185 4.287148794603087E-4 2.408510558765779E-6 4.937446645469847E-5 1.396936124084152E-4 34-35 0.17245056026290917 4.6484253784179533E-4 3.6127658381486686E-6 5.2987232292847134E-5 1.4210212296718097E-4 36-37 0.2358485794460214 4.913361539882189E-4 4.817021117531558E-6 6.502978508667604E-5 1.5294042048162696E-4 38-39 0.3338135421685401 5.250553018109399E-4 6.021276396914447E-6 6.98468062042076E-5 1.6739148383422163E-4 40-41 0.47293152055341026 5.467318968398317E-4 7.225531676297337E-6 7.586808260112204E-5 1.7341276023113609E-4 42-43 0.6568754932027496 5.647957260305752E-4 7.225531676297337E-6 7.827659315988782E-5 1.8665956830434785E-4 44-45 0.8907358471825099 5.88880831618233E-4 7.225531676297337E-6 7.948084843927071E-5 1.9629361053941096E-4 46-47 1.1818501099099707 6.153744477646565E-4 7.225531676297337E-6 8.06851037186536E-5 1.9990637637755965E-4 48-49 1.5317874260141684 6.43072319190463E-4 7.225531676297337E-6 8.188935899803649E-5 2.1194892917138856E-4 50-51 1.9397289019051223 6.659531694987379E-4 7.225531676297337E-6 8.188935899803649E-5 2.1676595028892012E-4 52-53 2.4028577844843557 6.852212539688641E-4 7.225531676297337E-6 8.791063539495093E-5 2.3001275836213191E-4 54-55 2.919955367409133 7.357999757029455E-4 9.634042235063116E-6 9.754467763001404E-5 2.3603403475904635E-4 56-57 3.489636757108165 7.671106129669006E-4 9.634042235063116E-6 1.0717871986507717E-4 2.540978639497897E-4 58-59 4.11129862168648 8.092595477453017E-4 9.634042235063116E-6 1.1079148570322584E-4 2.6614041674361856E-4 60-61 4.781653344231362 8.417744402886397E-4 9.634042235063116E-6 1.1079148570322584E-4 2.805914800962133E-4 62-63 5.491778597377865 8.586340142000002E-4 9.634042235063116E-6 1.1319999626199162E-4 2.914297776106593E-4 64-65 6.238314508896508 8.839233750670408E-4 9.634042235063116E-6 1.156085068207574E-4 2.986553092869566E-4 66-67 7.018786334188162 9.188467781691446E-4 9.634042235063116E-6 1.1801701737952317E-4 3.058808409632539E-4 68-69 7.831145635022596 9.453403943155683E-4 9.634042235063116E-6 1.1922127265890606E-4 3.131063726395513E-4 70-71 8.666854241468982 9.754467763001405E-4 9.634042235063116E-6 1.2042552793828895E-4 3.2274041487461433E-4 72-73 9.52929851937295 0.00101277868996101 9.634042235063116E-6 1.2162978321767184E-4 3.323744571096775E-4 74-75 10.411873128527082 0.0010513148589012624 9.634042235063116E-6 1.2644680433520342E-4 3.4682552046227217E-4 76-77 11.31334732003585 0.0010850340067239835 9.634042235063116E-6 1.3005957017335208E-4 3.49234031021038E-4 78-79 12.232326566285728 0.0011175488992673215 1.2042552793828894E-5 1.3126382545273498E-4 3.576638179767182E-4 80-81 13.16581106737577 0.0011464510259725108 1.2042552793828894E-5 1.372851018496494E-4 3.648893496530155E-4 82-83 14.112853074401107 0.0011741488973983172 1.2042552793828894E-5 1.408978676877981E-4 3.745233918880786E-4 84-85 15.069903647053394 0.0012114808110591868 1.2042552793828894E-5 1.4691914408471253E-4 3.901787105200562E-4 86-87 16.03681225342271 0.0012391786824849933 1.2042552793828894E-5 1.4691914408471253E-4 3.92587221078822E-4 88 16.762515752863308 0.0012596510222345025 1.2042552793828894E-5 1.493276546434783E-4 3.9740424219635354E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 267060 0.0 86.26395 8 AGTGGTA 270135 0.0 85.13152 5 GTATCAA 270450 0.0 85.03965 9 TGGTATC 270500 0.0 84.934784 7 GTGGTAT 271270 0.0 84.79244 6 AAGCAGT 281960 0.0 82.49248 1 CAGTGGT 283820 0.0 81.23994 4 GCAGTGG 286530 0.0 81.06522 3 AGCAGTG 294535 0.0 78.970215 2 CAACGCA 263105 0.0 45.1296 12-13 ATCAACG 265515 0.0 44.771214 10-11 ACGCAGA 268040 0.0 44.257023 14-15 TCAACGC 263060 0.0 44.004284 12-13 AGAGTAC 269470 0.0 43.509945 18-19 AACGCAG 265570 0.0 43.50482 14-15 GAGTACT 261745 0.0 43.025517 20-21 CGCAGAG 271065 0.0 42.53262 16-17 AGTACTT 272120 0.0 42.456226 20-21 TATCAAC 271910 0.0 42.33784 10-11 GTACTTT 271995 0.0 41.435516 22-23 >>END_MODULE