FastQCFastQC Report
Thu 26 May 2016
SRR1660573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41519436
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20372314.906692374144966No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589590.14200337403427155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2474900.085.993348
AGTGGTA2516000.084.6262055
GTGGTAT2517150.084.598746
GTATCAA2518000.084.36659
TGGTATC2523750.084.303027
AAGCAGT2620000.081.709941
CAGTGGT2656250.080.33314
GCAGTGG2668500.080.269023
AGCAGTG2740200.078.321342
CAACGCA2430950.045.00326512-13
ATCAACG2456900.044.53431310-11
ACGCAGA2481750.044.0972114-15
TCAACGC2426700.043.89581312-13
AACGCAG2461150.043.2297814-15
AGAGTAC2525400.043.11031318-19
GAGTACT2439550.042.83163520-21
CGCAGAG2506800.042.43018316-17
TATCAAC2530100.042.04863710-11
AGTACTT2549900.042.02408620-21
GTACTTT2548000.041.0245322-23
Produced by FastQC (version 0.11.5)