FastQCFastQC Report
Thu 26 May 2016
SRR1660572_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660572_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41176227
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22509865.466712625224258No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA521610.1266774636733958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2724600.086.237328
GTGGTAT2747250.085.3930746
AGTGGTA2756500.085.1871265
GTATCAA2755550.085.110089
TGGTATC2756500.085.0792547
AAGCAGT2880450.082.503451
GCAGTGG2937950.080.760363
CAGTGGT2914850.080.726524
AGCAGTG3013900.078.852692
CAACGCA2693250.044.9844112-13
ATCAACG2715750.044.65757410-11
ACGCAGA2749400.044.0471614-15
TCAACGC2693650.043.82237212-13
AGAGTAC2745800.043.46373718-19
AACGCAG2725000.043.2400714-15
GAGTACT2672850.042.92365320-21
CGCAGAG2771000.042.4621216-17
AGTACTT2782200.042.3374620-21
TATCAAC2781200.042.1742710-11
GTACTTT2766900.041.5329322-23
Produced by FastQC (version 0.11.5)