##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1660572_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41176227 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95060856352866 34.0 31.0 34.0 30.0 34.0 2 32.09666011895651 34.0 31.0 34.0 30.0 34.0 3 32.11971266818594 34.0 31.0 34.0 30.0 34.0 4 35.539548706101705 37.0 35.0 37.0 33.0 37.0 5 35.529719369382725 37.0 35.0 37.0 33.0 37.0 6 35.55081569275398 37.0 35.0 37.0 33.0 37.0 7 35.48644393766335 37.0 35.0 37.0 33.0 37.0 8 35.516260875480405 37.0 35.0 37.0 33.0 37.0 9 37.18871561495909 39.0 37.0 39.0 34.0 39.0 10-11 37.088307131685475 39.0 37.0 39.0 34.0 39.0 12-13 37.13963256031205 39.0 37.0 39.0 34.0 39.0 14-15 38.53618166618325 40.0 38.0 41.0 34.0 41.0 16-17 38.49557576025603 40.0 38.0 41.0 34.0 41.0 18-19 38.456573522387075 40.0 38.0 41.0 34.0 41.0 20-21 38.38542839294139 40.0 38.0 41.0 34.0 41.0 22-23 38.21442784206528 40.0 38.0 41.0 33.5 41.0 24-25 38.222461992450164 40.0 38.0 41.0 34.0 41.0 26-27 38.21669948050364 40.0 38.0 41.0 34.0 41.0 28-29 38.154425586394794 40.0 38.0 41.0 33.5 41.0 30-31 38.06713733145098 40.0 38.0 41.0 33.0 41.0 32-33 37.957196333214306 40.0 38.0 41.0 33.0 41.0 34-35 37.85277093260633 40.0 38.0 41.0 33.0 41.0 36-37 37.67095190630263 40.0 38.0 41.0 33.0 41.0 38-39 37.26049504244281 40.0 36.0 41.0 32.0 41.0 40-41 37.05784881650278 40.0 36.0 41.0 31.5 41.0 42-43 36.948256793901976 40.0 35.0 41.0 31.0 41.0 44-45 36.81672089577319 40.0 35.0 41.0 31.0 41.0 46-47 36.66027188455125 39.5 35.0 41.0 31.0 41.0 48-49 36.451400258212104 39.0 35.0 41.0 30.5 41.0 50-51 35.34381974822511 38.0 34.0 40.0 29.0 40.5 52-53 35.18748508696535 38.0 34.5 39.5 28.0 40.5 54-55 35.4788496405948 39.0 35.0 40.5 28.0 41.0 56-57 35.280542726753474 39.0 35.0 41.0 26.0 41.0 58-59 35.07674338399194 39.0 35.0 41.0 26.0 41.0 60-61 34.84686051978487 39.0 35.0 41.0 26.0 41.0 62-63 34.57220288784594 38.0 35.0 41.0 25.5 41.0 64-65 34.25536890497519 37.5 34.5 40.0 24.5 41.0 66-67 33.92932434047442 37.0 34.0 40.0 23.5 41.0 68-69 33.5804981840614 36.0 34.0 39.5 23.0 41.0 70-71 33.2126621630486 36.0 34.0 39.0 22.0 41.0 72-73 32.83528840561327 35.0 34.0 39.0 21.5 41.0 74-75 32.44695055474607 35.0 33.0 37.5 20.0 40.0 76-77 32.07241916555395 35.0 33.0 37.0 18.5 39.0 78-79 31.714312775670294 35.0 33.0 36.5 16.0 39.0 80-81 31.372039538736757 35.0 33.0 36.0 11.0 38.5 82-83 31.083128354620737 35.0 33.0 36.0 7.0 37.0 84-85 30.825371336232436 35.0 33.0 35.0 2.0 37.0 86-87 30.588042962265583 35.0 32.0 35.0 2.0 36.5 88-89 30.375613044876598 35.0 32.0 35.0 2.0 36.0 90-91 30.181188699003435 35.0 32.0 35.0 2.0 36.0 92-93 29.993152650921612 35.0 32.0 35.0 2.0 36.0 94-95 29.809366117978705 35.0 32.0 35.0 2.0 35.0 96-97 29.607466621456112 35.0 31.5 35.0 2.0 35.0 98-99 29.426688997027338 35.0 31.0 35.0 2.0 35.0 100 29.292634266855 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31583550951405925 1101 2 0.3038865976431424 1101 3 0.2732502984882146 1101 4 0.23081115656754747 1101 5 0.2351181099847537 1101 6 0.25740029897621497 1101 7 0.209596741958201 1101 8 0.21050680539324418 1101 9 0.34793411550523956 1101 10-11 0.34312582234460365 1101 12-13 0.34134347140259536 1101 14-15 0.430223320756852 1101 16-17 0.3978900998347754 1101 18-19 0.41246677711070845 1101 20-21 0.41517285651696767 1101 22-23 0.4228127016615417 1101 24-25 0.41058210784935767 1101 26-27 0.3832523885239283 1101 28-29 0.44120819061767946 1101 30-31 0.40260873213836845 1101 32-33 0.38559281717862604 1101 34-35 0.41055692856978965 1101 36-37 0.38190720038831927 1101 38-39 0.40005806167136626 1101 40-41 0.4983093575197657 1101 42-43 0.4464007189428685 1101 44-45 0.42040307715402037 1101 46-47 0.3971498910559248 1101 48-49 0.43229637966528855 1101 50-51 0.34870246830165996 1101 52-53 0.3700676077797027 1101 54-55 0.37607345321855945 1101 56-57 0.3627447502532206 1101 58-59 0.36769616775626446 1101 60-61 0.3976295897676678 1101 62-63 0.3527172561724399 1101 64-65 0.3190641271355332 1101 66-67 0.3521177946639753 1101 68-69 0.35809693740512216 1101 70-71 0.35029094136226746 1101 72-73 0.30251314597614254 1101 74-75 0.36403353891120815 1101 76-77 0.33088768310050654 1101 78-79 0.3312374555936408 1101 80-81 0.29795073794708316 1101 82-83 0.2918749760358992 1101 84-85 0.2585874887033235 1101 86-87 0.25763907276743936 1101 88-89 0.2317548871908066 1101 90-91 0.23591252032719012 1101 92-93 0.23803669013922857 1101 94-95 0.16843744805230543 1101 96-97 0.21243541171894975 1101 98-99 0.28943642597913666 1101 100 0.2743563268631526 1102 1 0.35230685895867 1102 2 0.3548305320128122 1102 3 0.3502759015870467 1102 4 0.326089704399692 1102 5 0.3150568299211258 1102 6 0.31129806936938564 1102 7 0.32826276508758667 1102 8 0.31438059911805283 1102 9 0.35331222299037535 1102 10-11 0.3908137375916141 1102 12-13 0.40720018538818437 1102 14-15 0.4300906819275667 1102 16-17 0.4820159840232847 1102 18-19 0.499888139142584 1102 20-21 0.47748455327739237 1102 22-23 0.48903046450059406 1102 24-25 0.4969609162264561 1102 26-27 0.46909106376281073 1102 28-29 0.48538290272028206 1102 30-31 0.49768223844217374 1102 32-33 0.47695555078849594 1102 34-35 0.5034480230759257 1102 36-37 0.4944763460474846 1102 38-39 0.5226268765538933 1102 40-41 0.5951618807475754 1102 42-43 0.516520752823368 1102 44-45 0.51157725545076 1102 46-47 0.49569974066479716 1102 48-49 0.5004657769105023 1102 50-51 0.4591168817307718 1102 52-53 0.4775053841716854 1102 54-55 0.5857631206385534 1102 56-57 0.5515922126561108 1102 58-59 0.5550290790356271 1102 60-61 0.5449097582229498 1102 62-63 0.5298321863472566 1102 64-65 0.5191803208297188 1102 66-67 0.5041209946492984 1102 68-69 0.5637028751160003 1102 70-71 0.5228759803892302 1102 72-73 0.5198071847096131 1102 74-75 0.4777570209145452 1102 76-77 0.44934319528010747 1102 78-79 0.5073458352731599 1102 80-81 0.46229422215402494 1102 82-83 0.4252608738105863 1102 84-85 0.4423324544444469 1102 86-87 0.4365785314705235 1102 88-89 0.4014698631941762 1102 90-91 0.34996906215146595 1102 92-93 0.32089134834417976 1102 94-95 0.37987060434724995 1102 96-97 0.4033798701054252 1102 98-99 0.4151174854862276 1102 100 0.40557612483521766 1103 1 0.3673114235629811 1103 2 0.3300079675366092 1103 3 0.3421559457508252 1103 4 0.3232069762754506 1103 5 0.31625996760205766 1103 6 0.30930020163864214 1103 7 0.33062507980140055 1103 8 0.338298461622756 1103 9 0.3828730553944766 1103 10-11 0.38185890275481427 1103 12-13 0.37841553169605646 1103 14-15 0.455920161938451 1103 16-17 0.4556069173756825 1103 18-19 0.5113037510861034 1103 20-21 0.4937289931965907 1103 22-23 0.4700333986819345 1103 24-25 0.48909381137307406 1103 26-27 0.44777712434628114 1103 28-29 0.4928283246042824 1103 30-31 0.4738890897553816 1103 32-33 0.44195138683534907 1103 34-35 0.4763976645817536 1103 36-37 0.4405581830055425 1103 38-39 0.4407035464639719 1103 40-41 0.5336915096503034 1103 42-43 0.49898978104604197 1103 44-45 0.4923981349394708 1103 46-47 0.4407598322210404 1103 48-49 0.5214737425623355 1103 50-51 0.4120571828000337 1103 52-53 0.45855501324256664 1103 54-55 0.5397301957379881 1103 56-57 0.5370817393597278 1103 58-59 0.5231874448706009 1103 60-61 0.5259346167126466 1103 62-63 0.5289966509482653 1103 64-65 0.4881334787452829 1103 66-67 0.45136371805808295 1103 68-69 0.4253289702318739 1103 70-71 0.42435723385030144 1103 72-73 0.41774761161864404 1103 74-75 0.4550008542682278 1103 76-77 0.4223288770435758 1103 78-79 0.4083465472037737 1103 80-81 0.39407672918350656 1103 82-83 0.3485283216586801 1103 84-85 0.37673675243355476 1103 86-87 0.3661057712386828 1103 88-89 0.3504666861604626 1103 90-91 0.38038693207768404 1103 92-93 0.3848053493615602 1103 94-95 0.41121427046874004 1103 96-97 0.46893517767404447 1103 98-99 0.48536631197989877 1103 100 0.4596355360927795 1104 1 0.2532196744309445 1104 2 0.27372030186919716 1104 3 0.2559081298583692 1104 4 0.212201085610765 1104 5 0.23231698907063247 1104 6 0.26038973510629404 1104 7 0.2807996262428176 1104 8 0.28776684936416785 1104 9 0.353937014273626 1104 10-11 0.3646359358722009 1104 12-13 0.3678344499777211 1104 14-15 0.40669139308492674 1104 16-17 0.35853475919577704 1104 18-19 0.36892024133064183 1104 20-21 0.3508156939841953 1104 22-23 0.3759362034009399 1104 24-25 0.3936594028407754 1104 26-27 0.356280306457073 1104 28-29 0.38868716848757145 1104 30-31 0.3896347470514101 1104 32-33 0.33241326955557327 1104 34-35 0.4040732789557424 1104 36-37 0.40142160223399515 1104 38-39 0.43502550274746454 1104 40-41 0.5004236691741042 1104 42-43 0.4217298198935566 1104 44-45 0.38339643470833096 1104 46-47 0.39885139278831616 1104 48-49 0.39818762510077477 1104 50-51 -0.016221265377716065 1104 52-53 0.2285444956738445 1104 54-55 0.3621365525291722 1104 56-57 0.40781418473282827 1104 58-59 0.40774190067213567 1104 60-61 0.3860851553056932 1104 62-63 0.3922294810607738 1104 64-65 0.36177284794982256 1104 66-67 0.3563980650956893 1104 68-69 0.3038226189292388 1104 70-71 0.2537370678959121 1104 72-73 0.3033551738425402 1104 74-75 0.26501277204584284 1104 76-77 0.2835508841390961 1104 78-79 0.25807346108145524 1104 80-81 0.25940163270795935 1104 82-83 0.23434501135608343 1104 84-85 0.20723212482960562 1104 86-87 0.2428267858901414 1104 88-89 0.25329982950747265 1104 90-91 0.21686940333094995 1104 92-93 0.2815495803797461 1104 94-95 0.2962662666532587 1104 96-97 0.23821251489441764 1104 98-99 0.2774737044917366 1104 100 0.23925371588544664 1105 1 0.218429405496245 1105 2 0.22280509861732156 1105 3 0.19608581062605168 1105 4 0.20799203012385448 1105 5 0.20904983955229994 1105 6 0.16889218968241693 1105 7 0.19118032700770016 1105 8 0.21113316527931403 1105 9 0.2409656968601368 1105 10-11 0.2749263926779264 1105 12-13 0.24585478894003643 1105 14-15 0.2668003719004588 1105 16-17 0.31418163345225736 1105 18-19 0.3092445756881119 1105 20-21 0.3125700932269311 1105 22-23 0.3540100717413708 1105 24-25 0.29178357754715734 1105 26-27 0.299607034681209 1105 28-29 0.29399081132389426 1105 30-31 0.3110258599989848 1105 32-33 0.33689749714736905 1105 34-35 0.32844818077406757 1105 36-37 0.3600003463919066 1105 38-39 0.33531809812756563 1105 40-41 0.43394674497741903 1105 42-43 0.36820769437387924 1105 44-45 0.3695600576710021 1105 46-47 0.34108643265787464 1105 48-49 0.3602463329277015 1105 50-51 0.30142958168485023 1105 52-53 0.36588204361516574 1105 54-55 0.4108776459594523 1105 56-57 0.4144885117357333 1105 58-59 0.3836609620829492 1105 60-61 0.340883693954396 1105 62-63 0.3238425639011595 1105 64-65 0.3149657557206069 1105 66-67 0.32233388716211664 1105 68-69 0.3228391912115214 1105 70-71 0.2939707428279661 1105 72-73 0.3003311114615812 1105 74-75 0.31809883111074555 1105 76-77 0.29396012667223914 1105 78-79 0.27793936913018413 1105 80-81 0.3107697630111197 1105 82-83 0.2815505940617271 1105 84-85 0.2918656845417509 1105 86-87 0.278768141359123 1105 88-89 0.2669489233406104 1105 90-91 0.2776359749211359 1105 92-93 0.3056621972484237 1105 94-95 0.30804842137255406 1105 96-97 0.28021268847425773 1105 98-99 0.28112340582159234 1105 100 0.2947764841837639 1106 1 0.12195109968744333 1106 2 0.1375210321818514 1106 3 0.10960076409569552 1106 4 0.07826173294292715 1106 5 0.10032445157472125 1106 6 0.11322663351148066 1106 7 0.1504787990331522 1106 8 0.16258356388482298 1106 9 0.13971554642666462 1106 10-11 0.1872404577295157 1106 12-13 0.1815738669291278 1106 14-15 0.1647497872216519 1106 16-17 0.13600542987322228 1106 18-19 0.16736295343183372 1106 20-21 0.20362290038628572 1106 22-23 0.22567602333539583 1106 24-25 0.1680783677490041 1106 26-27 0.172046673480871 1106 28-29 0.1590186133214857 1106 30-31 0.10785229340545754 1106 32-33 0.1884261280363475 1106 34-35 0.14779566382998865 1106 36-37 0.22257944087950676 1106 38-39 0.23957492516513668 1106 40-41 0.28726127011698566 1106 42-43 0.1854405261733234 1106 44-45 0.17842529562540932 1106 46-47 0.17261634444228235 1106 48-49 0.14486145809767947 1106 50-51 0.11097016773931756 1106 52-53 0.15649992238487442 1106 54-55 0.16654748828366195 1106 56-57 0.22452531658696984 1106 58-59 0.20090233390559575 1106 60-61 0.21880188320770344 1106 62-63 0.2318614929878393 1106 64-65 0.1992297918696977 1106 66-67 0.1578976261791709 1106 68-69 0.21023199483182253 1106 70-71 0.19691045488461612 1106 72-73 0.09780598942300145 1106 74-75 0.14204743068635395 1106 76-77 0.1420847972307726 1106 78-79 0.1950367953795542 1106 80-81 0.23741580417440744 1106 82-83 0.25320322121574534 1106 84-85 0.2432715210095111 1106 86-87 0.25805678100633145 1106 88-89 0.2982537072391658 1106 90-91 0.29736117295875175 1106 92-93 0.2511833742671037 1106 94-95 0.2786615594422237 1106 96-97 0.2480115560766727 1106 98-99 0.22880218992565204 1106 100 0.22158378028977666 1107 1 0.1624870553717983 1107 2 0.09651173183423367 1107 3 0.15383542668474348 1107 4 0.1706675627117491 1107 5 0.16972691451135802 1107 6 0.187355100093626 1107 7 0.118842922901365 1107 8 0.13726551898138695 1107 9 0.18133101232795212 1107 10-11 0.20217310338131256 1107 12-13 0.18179244854682963 1107 14-15 0.23844037125693518 1107 16-17 0.24683252470276074 1107 18-19 0.22440926516777893 1107 20-21 0.2901406528831245 1107 22-23 0.2973128058738226 1107 24-25 0.24690767413654413 1107 26-27 0.27724440071026635 1107 28-29 0.24640898431101022 1107 30-31 0.2221958495739642 1107 32-33 0.22819416957593575 1107 34-35 0.1847067975852994 1107 36-37 0.22326744712953683 1107 38-39 0.19586660991702587 1107 40-41 0.26589981331400026 1107 42-43 0.25313002063420953 1107 44-45 0.25125273653467417 1107 46-47 0.24148996162129777 1107 48-49 0.22114461130859553 1107 50-51 0.22591552102058898 1107 52-53 0.25676879735625135 1107 54-55 0.24957705833936217 1107 56-57 0.25811835289755436 1107 58-59 0.3149834589087064 1107 60-61 0.31715225124946755 1107 62-63 0.3252695960560814 1107 64-65 0.29870199229893757 1107 66-67 0.2955221925763851 1107 68-69 0.28442531788666514 1107 70-71 0.2650890978318188 1107 72-73 0.2593471581207254 1107 74-75 0.2645439541560748 1107 76-77 0.2592657646270524 1107 78-79 0.2888130377809581 1107 80-81 0.23847902133755028 1107 82-83 0.2599263566145744 1107 84-85 0.284243797537016 1107 86-87 0.23738763951861586 1107 88-89 0.2878251047686966 1107 90-91 0.2800082582730319 1107 92-93 0.22370228499144673 1107 94-95 0.26602298757790166 1107 96-97 0.2428747541561087 1107 98-99 0.2792320392851053 1107 100 0.333285252306851 1108 1 0.1193070378705059 1108 2 0.14069107447683393 1108 3 0.1378767376666019 1108 4 0.11928209426133662 1108 5 0.08458276582129542 1108 6 0.12873711556726875 1108 7 0.10130173112718666 1108 8 0.09949356554463407 1108 9 0.1618956420383455 1108 10-11 0.1743777750270823 1108 12-13 0.1945380745322467 1108 14-15 0.23819850623810623 1108 16-17 0.220166549525743 1108 18-19 0.17520020599569364 1108 20-21 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-0.9786196873488677 2316 66-67 -1.0219788458744148 2316 68-69 -1.0938843203294368 2316 70-71 -1.0115786305484562 2316 72-73 -1.088745205656668 2316 74-75 -1.0479554496617105 2316 76-77 -1.0106364262751697 2316 78-79 -0.9776860713296891 2316 80-81 -1.0182760052613524 2316 82-83 -1.0234429178581763 2316 84-85 -1.0528750506698898 2316 86-87 -1.040427976494822 2316 88-89 -1.0451086099794153 2316 90-91 -1.03542308229245 2316 92-93 -1.0593489028714203 2316 94-95 -1.0561528205309934 2316 96-97 -1.083774348444006 2316 98-99 -1.0565965161841397 2316 100 -1.118457304139831 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 311467.0 3 43630.0 4 35954.0 5 36769.0 6 37677.0 7 40209.0 8 47096.0 9 59066.0 10 71505.0 11 84502.0 12 91000.0 13 95297.0 14 93568.0 15 88893.0 16 103247.0 17 134169.0 18 185378.0 19 285391.0 20 551881.0 21 672179.0 22 297496.0 23 240871.0 24 264478.0 25 287887.0 26 313058.0 27 357289.0 28 423315.0 29 510648.0 30 621398.0 31 773739.0 32 986475.0 33 1286136.0 34 1762359.0 35 2681841.0 36 4620616.0 37 8710730.0 38 1.0730891E7 39 3227919.0 40 10203.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 17.427898023063918 33.40114529037652 26.92160520184954 22.24935148471002 2 22.071261866475158 33.1090374403493 25.449376899315485 19.370323793860056 3 26.94930455748723 24.764216225343564 25.98909441445422 22.297384802714987 4 20.764410794419312 26.6249613987795 24.476275496783195 28.134352310017995 5 21.97324727150608 31.659434096590555 25.34962414909071 21.01769448281266 6 28.18085003783542 26.13270014345572 25.05744652518014 20.629003293528726 7 21.647130225750022 25.52599271551631 31.386718555518506 21.440158503215166 8 27.591846491249054 26.001832282450216 25.091131412433004 21.315189813867722 9 27.14899198833883 25.22491917929012 26.007692278920814 21.618396553450232 10-11 21.265734355582257 29.058708570818737 28.417826772918804 21.257730300680198 12-13 21.210910415006374 26.00901267147499 28.36979976623218 24.410277147286454 14-15 21.214936552550963 32.10207747768579 25.349721212074105 21.333264757689136 16-17 24.392362755811785 25.83156470826115 25.378758067150525 24.39731446877654 18-19 21.31700112722289 28.908403972184928 25.34483476352134 24.429760137070847 20-21 24.414407420957794 28.951901409573814 25.30307013387994 21.33062103558845 22-23 24.39179870974714 25.949553239451294 28.315372743602673 21.343275307198898 24-25 21.38694584560879 28.833327790078783 25.19160990962941 24.58811645468302 26-27 21.43361376052294 25.816609480481663 31.350515148055663 21.399261610939732 28-29 21.442387075007268 25.896707450838928 31.31929984028325 21.34160563387055 30-31 21.413282407414773 25.86625126159168 31.303867325081704 21.41659900591184 32-33 21.461819338592818 25.934066563978448 31.183152499101823 21.420961598326915 34-35 21.458496587430233 26.042603620603145 31.116933818640103 21.381965973326523 36-37 21.426499114453307 26.014839739991885 31.140870972604894 21.41779017294991 38-39 21.464660307992187 26.00256746381205 31.149422065610693 21.38335016258507 40-41 21.541473693854424 26.05964428645554 31.089016991339154 21.30986502835088 42-43 21.484624381997882 26.06360504714206 31.083639789880436 21.36813078097962 44-45 21.52110369879115 26.120491054968113 31.05545288668064 21.302952359560095 46-47 21.543154529707362 26.2256955972271 30.969433295253783 21.261716577811754 48-49 21.629596794051057 26.23235721592032 30.85567612847478 21.282369861553843 50-51 21.669923118366878 26.309704927197146 30.782293824758145 21.238078129677827 52-53 21.79545371558182 26.440966956192547 30.535859563117047 21.227719765108585 54-55 22.129772901581575 26.709256740825865 29.80402319785142 21.356947159741136 56-57 22.606192654455786 27.21192463296884 28.736258966433226 21.445623746142147 58-59 22.7376618101115 27.67049304812489 28.150654731264108 21.4411904104995 60-61 22.83377632897539 27.858803576808356 27.89321147303615 21.4142086211801 62-63 22.941408812990062 27.98626469906111 27.73379898193192 21.338527506016913 64-65 23.06733397563988 28.03718714426648 27.70545722927028 21.19002165082336 66-67 23.1573997675663 27.851938471877574 27.86157169539354 21.129090065162586 68-69 23.27302440829881 27.69383301913099 28.062977355790743 20.970165216779456 70-71 23.424561892813742 27.614156948687157 28.193439770943307 20.767841387555794 72-73 23.58807565051027 27.540622955882064 28.193883305696488 20.677418087911178 74-75 23.83208762449807 27.605517356926907 28.020511827039396 20.541883191535632 76-77 24.109822681203262 27.744908172498604 27.689595712113558 20.455673434184575 78-79 24.3728039443085 27.876999022423536 27.3401067440632 20.410090289204764 80-81 24.614817886753677 28.065757069455817 26.99282885772026 20.32659618607025 82-83 24.8139076394313 28.20947288562939 26.71717457470775 20.259444900231564 84-85 24.997153629016434 28.228912617303425 26.539688139268357 20.23424561441178 86-87 25.20253822814178 28.217342980196857 26.41828009066523 20.16183870099613 88-89 25.37964213186685 28.229157040857096 26.304382942275844 20.08681788500021 90-91 25.58216785078795 28.161699292672647 26.20050773402436 20.055625122515043 92-93 25.748804964225315 28.14868080807109 26.11399641308314 19.988517814620455 94-95 25.89916557093032 28.118754110035983 26.063741281872048 19.918339037161655 96-97 26.027988579438926 28.02985362976427 26.00843234172047 19.933725449076338 98-99 26.164892790546535 28.00701313211028 25.970610503380758 19.857483573962426 100 26.23171751478187 28.042512161120108 25.88613029461345 19.839640029484563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 62782.0 1 42297.5 2 18013.0 3 13425.5 4 12504.5 5 12302.0 6 12545.0 7 13098.0 8 13174.0 9 13195.0 10 13729.0 11 17119.0 12 213555.5 13 255294.0 14 100725.5 15 103246.0 16 114403.0 17 124656.0 18 134131.5 19 143901.0 20 154710.5 21 170301.5 22 192397.5 23 219321.0 24 247841.0 25 278382.5 26 313722.0 27 357320.5 28 409135.5 29 461839.0 30 511664.5 31 563079.0 32 620015.5 33 676855.0 34 734063.0 35 799949.5 36 871343.5 37 931664.0 38 993186.5 39 1055478.5 40 1098072.5 41 1145984.0 42 1202786.5 43 1247424.5 44 1286345.0 45 1331470.0 46 1380492.0 47 1422710.0 48 1457721.0 49 1487259.0 50 1502051.0 51 1499852.5 52 1483795.0 53 1451357.5 54 1398579.5 55 1327138.5 56 1242634.0 57 1136821.5 58 1009805.5 59 875990.0 60 732532.0 61 590746.0 62 465544.0 63 355917.5 64 267708.5 65 200119.0 66 148137.0 67 109982.5 68 81727.0 69 61970.0 70 47998.0 71 37596.0 72 30110.0 73 23258.5 74 17808.5 75 14133.0 76 10794.5 77 7261.0 78 4883.0 79 3492.0 80 2456.5 81 1770.0 82 1332.0 83 1005.5 84 803.5 85 697.0 86 557.0 87 442.0 88 370.0 89 327.0 90 278.0 91 234.0 92 195.0 93 153.0 94 140.0 95 123.0 96 103.0 97 88.5 98 68.0 99 54.5 100 34.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006226893979382812 2 0.009296626424757178 3 0.012218215136612686 4 0.01274764684000795 5 0.01565223545129572 6 0.0044880265498827765 7 0.0025184434698205835 8 0.005935463683935879 9 0.0072687572856056 10-11 0.008126048071378662 12-13 0.009109625318512062 14-15 0.012499931088878055 16-17 0.021019410059110077 18-19 0.0350869932789131 20-21 0.028223809821137813 22-23 0.03867037162001268 24-25 1.3478651164420676E-4 26-27 0.006497681295568922 28-29 0.009626914092930369 30-31 0.010992993602837871 32-33 0.012933433653355371 34-35 0.010850921333807492 36-37 0.014320156142523695 38-39 0.011798069794010024 40-41 0.011754355249692984 42-43 0.002438300138572677 44-45 0.004999243859812605 46-47 0.007792117524512384 48-49 0.014303156041955957 50-51 0.011232209303683894 52-53 0.0016769384917175631 54-55 0.009421698593219821 56-57 0.015273376067214708 58-59 0.01414408367235784 60-61 0.016752384816607895 62-63 0.01765824731828878 64-65 0.016737813301835547 66-67 0.015075446324890331 68-69 0.00901976764408259 70-71 0.0018068678317709876 72-73 0.011511496670153873 74-75 0.012034856909060658 76-77 0.01573723595413441 78-79 0.007229899912879341 80-81 0.001551866323254921 82-83 0.009380412634698172 84-85 0.013069434457897272 86-87 0.012111357361615479 88-89 0.013229721120393086 90-91 0.012778004162450337 92-93 0.01334022177408338 94-95 0.011906141861904928 96-97 0.012677217851941605 98-99 0.013269792786017037 100 0.01179078403662385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4.1176227E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.184989713855714 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41410464716773 34.8299964740908 2 11.929535910048806 11.019309865933527 3 4.383220615053282 6.073169970593883 4 2.290583551179116 4.231623109997383 5 1.3558500034878285 3.1309959232308295 6 0.903286411245991 2.503096416723704 7 0.6428529606202869 2.078311016163875 8 0.4690703748658686 1.7331208350603668 9 0.36475329081467944 1.5161514285933895 >10 2.018720615174554 17.128973745807112 >50 0.171809021348905 5.543952069116576 >100 0.05504452267533456 3.7946124720903045 >500 7.818066650544811E-4 0.24067905379904983 >1k 3.338524371703624E-4 0.3599599177681877 >5k 3.1450334760322034E-5 0.09829050317919359 >10k+ 2.0966880705822437E-5 5.71775719785182 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2250986 5.466712625224258 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52161 0.1266774636733958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0018457252044972454 1.2142928976955562E-5 0.0 2.4285857953911126E-6 2.4285857953911126E-6 2 0.0018942969204050677 2.4285857953911124E-5 0.0 4.857171590782225E-6 9.71434318156445E-6 3 0.001984154594834539 3.88573727262578E-5 0.0 4.857171590782225E-6 1.4571514772346675E-5 4 0.002044869239719317 4.614313011243114E-5 0.0 4.857171590782225E-6 1.94286863631289E-5 5 0.0020691550976732276 5.82860590893867E-5 0.0 4.857171590782225E-6 1.94286863631289E-5 6 0.0021055838846040947 6.314323068016892E-5 0.0 4.857171590782225E-6 1.94286863631289E-5 7 0.00211772681358105 7.042898806634226E-5 0.0 4.857171590782225E-6 2.6714443749302236E-5 8 0.002159012772102699 8.500050283868893E-5 0.0 4.857171590782225E-6 3.157161534008446E-5 9 0.0021954415590335658 8.742908863408005E-5 0.0 4.857171590782225E-6 3.157161534008446E-5 10-11 0.0022646562542022125 1.0685777499720896E-4 0.0 4.857171590782225E-6 3.400020113547557E-5 12-13 0.0023435852925524237 1.275007542580334E-4 0.0 4.857171590782225E-6 4.492883721473558E-5 14-15 0.0024407287243680678 1.469294406211623E-4 0.0 8.500050283868894E-6 5.464318039630003E-5 16-17 0.00252208634851367 1.602866624958134E-4 0.0 9.71434318156445E-6 5.82860590893867E-5 18-19 0.002618015487431619 1.7607247016585565E-4 0.0 9.71434318156445E-6 6.192893778247337E-5 20-21 0.002704230283168004 1.9914403522207122E-4 0.0 9.71434318156445E-6 6.92146951686467E-5 22-23 0.002798945129188257 2.1128696419902678E-4 0.0 1.2142928976955562E-5 7.892903835021115E-5 24-25 0.003100089767816755 2.4042999374372014E-4 0.0 1.2142928976955562E-5 8.500050283868893E-5 26-27 0.003963452018078296 2.6107297300454455E-4 0.0 1.700010056773779E-5 8.985767442947116E-5 28-29 0.006545038718579048 2.744301948791957E-4 0.0 1.700010056773779E-5 1.080720678949045E-4 30-31 0.013636509241121097 2.8535883095845573E-4 0.0 1.700010056773779E-5 1.2264358266725117E-4 32-33 0.030147249771087573 3.0114463862849795E-4 0.0 1.700010056773779E-5 1.3357221874651118E-4 34-35 0.06104250396715562 3.1207327470775796E-4 0.0 2.1857272158520012E-5 1.4571514772346674E-4 36-37 0.11402938885099889 3.3878771845706024E-4 0.0 2.4285857953911124E-5 1.5300090510964007E-4 38-39 0.19470215180230088 3.7278791959253577E-4 0.0 2.914302954469335E-5 1.5907236959811786E-4 40-41 0.310226335210363 3.88573727262578E-4 0.0 2.914302954469335E-5 1.6514383408659566E-4 42-43 0.4659436135321481 4.0921670652340245E-4 0.0 3.157161534008446E-5 1.7364388437046455E-4 44-45 0.6666018719976456 4.335025644773136E-4 0.0 3.157161534008446E-5 1.7971534885894233E-4 46-47 0.9177516434422222 4.6143130112431135E-4 0.0 3.278590823778002E-5 1.8335822755202898E-4 48-49 1.221760799016384 4.832885732828314E-4 0.0 3.6428786930866686E-5 1.9550115652898454E-4 50-51 1.578257765093436 5.015029667482648E-4 2.4285857953911126E-6 4.128595852164891E-5 2.0035832811976677E-4 52-53 1.9913019228303748 5.391460465768269E-4 2.4285857953911126E-6 4.73574230101267E-5 2.137155499944179E-4 54-55 2.457365751359395 5.707176619169114E-4 2.4285857953911126E-6 5.464318039630003E-5 2.246441860736779E-4 56-57 2.97686332455861 5.950035198708226E-4 2.4285857953911126E-6 5.950035198708226E-5 2.2950135766446013E-4 58-59 3.544466325192932 6.241465494155159E-4 2.4285857953911126E-6 6.314323068016892E-5 2.355728221529379E-4 60-61 4.1598760372095285 6.532895789602092E-4 2.4285857953911126E-6 6.435752357786449E-5 2.38001407948329E-4 62-63 4.816009490136141 6.727182653233382E-4 2.4285857953911126E-6 7.042898806634226E-5 2.5378721561837125E-4 64-65 5.51606998863689 6.970041232772493E-4 2.4285857953911126E-6 7.285757386173337E-5 2.7321590198150017E-4 66-67 6.248135605042201 7.164328096403781E-4 2.4285857953911126E-6 7.77147454525156E-5 2.792873664699779E-4 68-69 7.012847486002057 7.285757386173337E-4 2.4285857953911126E-6 8.01433312479067E-5 2.9871605283310684E-4 70-71 7.807078827304892 7.46790132082767E-4 2.4285857953911126E-6 8.378620994099338E-5 3.096446889123668E-4 72-73 8.628138755889411 7.650045255482005E-4 2.4285857953911126E-6 8.742908863408005E-5 3.217876178893224E-4 74-75 9.47091509865632 7.77147454525156E-4 2.4285857953911126E-6 8.864338153177561E-5 3.242162036847135E-4 76-77 10.332582196032677 7.99004726683676E-4 3.642878693086669E-6 9.957201761103561E-5 3.290733752754957E-4 78-79 11.212860760652013 8.172191201491093E-4 4.857171590782225E-6 1.0200060340642673E-4 3.363591326616691E-4 80-81 12.10991842453171 8.342192207168471E-4 4.857171590782225E-6 1.0564348209951339E-4 3.472877687409291E-4 82-83 13.021622889343407 8.463621496938027E-4 4.857171590782225E-6 1.1292923948568674E-4 3.582164048201891E-4 84-85 13.945341568084906 8.694337147500183E-4 4.857171590782225E-6 1.1535782528107784E-4 3.7278791959253577E-4 86-87 14.881473963119545 8.900766940108428E-4 4.857171590782225E-6 1.190007039741645E-4 3.8007367697870907E-4 88 15.586816635725269 9.05862501680885E-4 4.857171590782225E-6 1.190007039741645E-4 3.837165556717958E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 272460 0.0 86.23732 8 GTGGTAT 274725 0.0 85.393074 6 AGTGGTA 275650 0.0 85.187126 5 GTATCAA 275555 0.0 85.11008 9 TGGTATC 275650 0.0 85.079254 7 AAGCAGT 288045 0.0 82.50345 1 GCAGTGG 293795 0.0 80.76036 3 CAGTGGT 291485 0.0 80.72652 4 AGCAGTG 301390 0.0 78.85269 2 CAACGCA 269325 0.0 44.98441 12-13 ATCAACG 271575 0.0 44.657574 10-11 ACGCAGA 274940 0.0 44.04716 14-15 TCAACGC 269365 0.0 43.822372 12-13 AGAGTAC 274580 0.0 43.463737 18-19 AACGCAG 272500 0.0 43.24007 14-15 GAGTACT 267285 0.0 42.923653 20-21 CGCAGAG 277100 0.0 42.46212 16-17 AGTACTT 278220 0.0 42.33746 20-21 TATCAAC 278120 0.0 42.17427 10-11 GTACTTT 276690 0.0 41.53293 22-23 >>END_MODULE