FastQCFastQC Report
Thu 26 May 2016
SRR1660572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1660572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41176227
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21051425.112517958481237No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA556910.13525037153112643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2543800.085.918128
AGTGGTA2577550.084.855295
GTATCAA2572950.084.796769
GTGGTAT2581700.084.6934056
TGGTATC2585350.084.512037
AAGCAGT2691200.081.672041
CAGTGGT2739200.080.038134
GCAGTGG2752500.079.92973
AGCAGTG2828650.077.894222
CAACGCA2501750.044.8774812-13
ATCAACG2526600.044.45710810-11
ACGCAGA2556950.043.90845514-15
TCAACGC2500700.043.75455512-13
AGAGTAC2590400.043.17127618-19
AACGCAG2532700.043.1421214-15
GAGTACT2501300.042.8965820-21
CGCAGAG2578750.042.3568716-17
AGTACTT2608100.042.18430320-21
TATCAAC2606550.041.93408610-11
GTACTTT2597500.041.34369322-23
Produced by FastQC (version 0.11.5)