FastQCFastQC Report
Sat 31 Dec 2022
SRR1645104.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645104.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011676
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC115281.1394952534210558No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.7491528908464765No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG60320.596238321359803No Hit
ACTCTGGACGAGGCGGAGCGCCAGTGGAAGGCCGAGTTCCACCGCTGGAGCT20080.19848251811844903No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT16760.16566568743352617Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT16290.16101993128234732No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA14300.14134960204650499No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT13430.1327500108730463No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA10830.10705008322822722No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCTTAACATCG10250.10131702244592143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)