FastQCFastQC Report
Sat 31 Dec 2022
SRR1645101.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645101.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595942
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT43030.7220501323954345RNA PCR Primer, Index 38 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT32840.5510603380865923RNA PCR Primer, Index 38 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT31360.5262257065284877RNA PCR Primer, Index 27 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC15470.2595890204080263RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT14460.2426410623852657RNA PCR Primer, Index 27 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC13960.23425098415617626RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.1968312352544375No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC11580.19431421178571068RNA PCR Primer, Index 38 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT11330.190119172671166RNA PCR Primer, Index 27 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC10580.1775340553275319RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG9560.16041829574018948No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8550.14347033771742887No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT8120.13625487044041198RNA PCR Primer, Index 27 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC7990.13407345010084873RNA PCR Primer, Index 38 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT6980.11712549207808813RNA PCR Primer, Index 38 (95% over 21bp)
ATAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT6800.11410506391561594RNA PCR Primer, Index 38 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)