FastQCFastQC Report
Sat 31 Dec 2022
SRR1645099.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645099.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892632
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT32830.36778874160908415RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT25390.2844397243208847RNA PCR Primer, Index 14 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCTT24240.2715564756809077RNA PCR Primer, Index 16 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21470.2405246506959195No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18270.2046756110020703No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15740.17633246399412075No Hit
ACCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC10740.12031833947248138RNA PCR Primer, Index 14 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.11886197223491876No Hit
CGCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGC10530.11796574624257253RNA PCR Primer, Index 16 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC10510.11774168974448597No Hit
TCTGTCTCTTATACACATCTGACGCAACCCATGTCGTATGCCGTCTTCTGCT10470.11729357674831287RNA PCR Primer, Index 14 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)