FastQCFastQC Report
Sat 31 Dec 2022
SRR1645098.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645098.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178732
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT44000.3732824764238181Illumina Single End Adapter 2 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34240.2904816361988985No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC28600.2426336096754818No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT28580.24246393582256187Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT26270.22286660581031142Illumina Single End Adapter 2 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23690.20097867878364206No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.14065962407061147No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC15470.13124272523355607No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC14950.1268312050576382Illumina Single End Adapter 2 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC12740.10808224430998734Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)