FastQCFastQC Report
Sat 31 Dec 2022
SRR1645097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810191
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT22550.2783294309613412Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC17090.2109379146398812No Hit
CTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCTT16710.2062476625882045Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGGACTGGTCGTATGCCGTCTTCTGCT15630.19291747254659702Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG11930.14724922888553438No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9450.11663916286406538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)