FastQCFastQC Report
Sat 31 Dec 2022
SRR1645096.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1645096.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288450
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT10290.3567342693707748No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT9140.3168660079736523No Hit
CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCTT8390.29086496793205063Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT4710.16328653146125846No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC4520.15669960131738603No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT3140.10885768764083896No Hit
ACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT3010.10435084070029468No Hit
AGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC2990.10365747963251863No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)