FastQCFastQC Report
Thu 26 May 2016
SRR1548184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548184_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2223311
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262101.1788724114620042No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGC253.8919858E-545.020
CGTTTTT139400.043.4505041
TACGGGA11450.041.462884
CGTTAGG3500.040.52
GTTAGCG1350.040.0000041
ACGGGAT13550.039.5202985
ACGGGAC9650.039.4041445
TGTCGCG403.4601726E-739.3751
TATAGCG1150.039.1304361
CGGGATA5600.038.9732136
AGGGATC30950.038.675286
TTGCGAG2800.038.5714261
TAGGGAC27100.038.5239875
CACGGGA8500.038.1176454
TATCGCG659.094947E-1238.076921
TAGGGAT48100.038.0301485
CTCACGA1600.037.9687524
CGAGGGA17900.037.9608964
TAAGGGA34200.037.9605264
ATAGGGA37900.037.8166244
Produced by FastQC (version 0.11.5)