##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548184_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2223311
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126109662570823	31.0	31.0	33.0	30.0	34.0
2	31.509934507588007	31.0	31.0	34.0	30.0	34.0
3	31.536221878090828	31.0	31.0	34.0	30.0	34.0
4	35.38314252931776	37.0	35.0	37.0	33.0	37.0
5	35.25386641814843	37.0	35.0	37.0	33.0	37.0
6	35.30430740458712	37.0	35.0	37.0	32.0	37.0
7	35.783744604331105	37.0	35.0	37.0	35.0	37.0
8	35.79469314009601	37.0	35.0	37.0	35.0	37.0
9	37.527162416773905	39.0	37.0	39.0	35.0	39.0
10	36.80561109084604	39.0	37.0	39.0	32.0	39.0
11	36.46687170620754	39.0	35.0	39.0	32.0	39.0
12	36.13092905131131	38.0	35.0	39.0	31.0	39.0
13	35.98995012393678	38.0	35.0	39.0	31.0	39.0
14	36.984433576769064	39.0	35.0	41.0	31.0	41.0
15	37.16597722945643	39.0	35.0	41.0	32.0	41.0
16	37.21809724325567	39.0	35.0	41.0	32.0	41.0
17	37.071433551131626	39.0	35.0	41.0	31.0	41.0
18	36.95073518729499	39.0	35.0	40.0	31.0	41.0
19	36.85159341180788	38.0	35.0	40.0	31.0	41.0
20	36.709117617823146	38.0	35.0	40.0	31.0	41.0
21	36.585228067508325	38.0	35.0	40.0	31.0	41.0
22	36.51109583859388	38.0	35.0	40.0	31.0	41.0
23	36.43694876695163	38.0	35.0	40.0	30.0	41.0
24	36.27171817168178	38.0	35.0	40.0	30.0	41.0
25	36.15391324020796	38.0	35.0	40.0	30.0	41.0
26	36.090044982460846	38.0	35.0	40.0	30.0	41.0
27	36.063799891243285	38.0	35.0	40.0	30.0	41.0
28	36.06802062329562	38.0	35.0	40.0	30.0	41.0
29	36.073140914608885	38.0	35.0	40.0	30.0	41.0
30	35.97637487512993	38.0	35.0	40.0	30.0	41.0
31	35.82843560797387	38.0	34.0	40.0	29.0	41.0
32	35.638606114933985	38.0	34.0	40.0	29.0	41.0
33	35.45740564410467	38.0	34.0	40.0	27.0	41.0
34	35.21834552161169	38.0	34.0	40.0	26.0	41.0
35	34.99851392810093	38.0	34.0	40.0	25.0	41.0
36	34.82735208884407	38.0	34.0	40.0	24.0	41.0
37	34.73743889181495	38.0	34.0	40.0	24.0	41.0
38	34.63868212769154	38.0	33.0	40.0	23.0	41.0
39	34.60131983334765	38.0	33.0	40.0	23.0	41.0
40	34.49107614724166	38.0	33.0	40.0	23.0	41.0
41	34.42309960234983	37.0	33.0	40.0	23.0	41.0
42	34.38253622637589	37.0	33.0	40.0	23.0	41.0
43	34.28090671975266	37.0	33.0	40.0	23.0	41.0
44	34.134897906770576	37.0	33.0	40.0	22.0	41.0
45	34.04612085308803	37.0	33.0	40.0	23.0	41.0
46	33.93162180189816	37.0	33.0	40.0	22.0	41.0
47	33.90143619133806	37.0	33.0	40.0	22.0	41.0
48	33.77484031698669	37.0	33.0	40.0	22.0	41.0
49	33.65073397288998	36.0	32.0	40.0	20.0	41.0
50	33.534180778127755	36.0	32.0	40.0	20.0	41.0
51	33.21469331101227	36.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	0.0
11	1.0
12	10.0
13	8.0
14	33.0
15	76.0
16	219.0
17	549.0
18	1237.0
19	2424.0
20	4169.0
21	6877.0
22	10105.0
23	14791.0
24	21644.0
25	30911.0
26	41858.0
27	49161.0
28	52582.0
29	56972.0
30	65561.0
31	78469.0
32	94482.0
33	117731.0
34	170702.0
35	216055.0
36	181455.0
37	222611.0
38	318476.0
39	464063.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.78822530900985	23.99623804317075	28.85264364724503	16.36289300057437
2	32.42137514724661	25.620527222687244	29.33300829258705	12.625089337479103
3	27.079522388005994	25.314272272300187	34.53210099711646	13.074104342577353
4	24.376256852954896	27.492869868407972	33.89121000165969	14.239663276977446
5	22.63300096117907	31.437122381888994	32.518527547428135	13.411349109503798
6	21.007632310549447	39.807206459195314	29.107443807906314	10.07771742234892
7	86.92207253056365	2.8848865498349086	8.865831186010414	1.3272097335910271
8	87.94860458118545	2.0592710601440825	8.461704188033073	1.5304201706373963
9	83.1119443028888	5.096542948782244	9.723740853168989	2.0677718951599666
10	37.8296153799446	39.02872787477775	14.502649426913283	8.639007318364367
11	27.610532219738936	23.6515269343785	32.11691931538143	16.62102153050113
12	28.361889092439156	22.571920887361237	31.680588095862433	17.38560192433717
13	22.702042134456224	27.272207981699363	32.37234916752537	17.65340071631904
14	17.366171444300864	31.44652277616582	32.945008593039844	18.242297186493477
15	17.023259454030498	27.736965273864072	39.470321515973254	15.769453756132181
16	21.082610574948806	25.40485788987685	37.63049793753551	15.882033597638836
17	20.86910018436467	24.83017445602527	32.46468892566087	21.836036433949186
18	21.35207355156341	26.092166143198142	34.18442134276311	18.371338962475335
19	24.027812573229745	27.684700880803447	30.739379241140803	17.548107304826
20	25.569162388887563	27.85354815408191	30.33439766186557	16.242891795164958
21	23.641721738434253	26.647778920717798	32.383458724397975	17.32704061644997
22	22.162261599929113	23.973794039610294	33.06127662751635	20.802667732944244
23	19.24260708465887	28.04870753574286	32.530626619487784	20.178058760110485
24	19.84994451968258	26.64247152107825	35.783882686677664	17.72370127256151
25	20.059361915629438	28.622806256074835	32.39686215738599	18.920969670909738
26	18.915932139048472	31.73411187188837	30.612136583680826	18.737819405382332
27	18.362748171533358	30.68599039900401	33.07553464180225	17.875726787660387
28	17.274056575980598	29.412664265143295	35.354927853098374	17.958351305777732
29	18.269508854136916	27.274771725593045	35.27288804850064	19.182831371769403
30	20.045598658937053	27.693831407301992	33.80215363482662	18.45841629893434
31	22.78920942684132	27.658928507977514	31.31001465831816	18.241847406863005
32	23.573355234602804	27.85228877111659	30.951990072463996	17.62236592181661
33	22.459970737337244	28.01115093659861	30.669663398417946	18.8592149276462
34	19.41730149313344	29.292483147881693	31.963139659723716	19.327075699261147
35	19.3428179863276	28.49929676954776	31.967097720471855	20.190787523652787
36	22.237644665995894	29.694676093448013	29.540806481864212	18.52687275869188
37	20.22164240630303	30.59657420846656	30.63543516853918	18.546348216691232
38	19.90234384663234	30.907821712751836	28.85790606892153	20.33192837169429
39	19.308904602190157	29.865727286915778	29.992610120671376	20.832757990222692
40	21.18264156476534	28.361754158550017	29.978666952126805	20.476937324557834
41	18.41181912921764	28.15944328076459	30.725076248891853	22.703661341125915
42	20.597073463856383	28.496822981580173	29.372903745809744	21.5331998087537
43	20.711812247589293	28.417976612358775	29.73020868425515	21.140002455796782
44	19.79875959773509	30.173826333787762	29.811169017739758	20.216245050737392
45	19.210537797006356	31.090252330870488	29.087518570276494	20.611691301846662
46	20.611331478142283	30.399030994764114	29.13384587221491	19.855791654878693
47	20.203426331268997	29.31857036644896	29.85511248763668	20.622890814645363
48	20.397956021447293	28.022035603655986	30.978212224920398	20.60179614997632
49	20.03075593113154	27.636664416269248	31.275336648808917	21.057243003790294
50	18.68321615824327	30.446662657630895	30.368715847670437	20.501405336455402
51	18.92033997942708	29.643940951130993	30.056029048567655	21.37969002087427
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1216.0
1	2003.5
2	2791.0
3	21428.5
4	40066.0
5	27801.0
6	15536.0
7	15370.0
8	15204.0
9	15920.5
10	16637.0
11	17146.5
12	17656.0
13	17584.0
14	17512.0
15	16902.0
16	16292.0
17	15428.0
18	14564.0
19	14066.0
20	13568.0
21	14452.5
22	15337.0
23	16631.0
24	17925.0
25	20437.0
26	27269.5
27	31590.0
28	37273.0
29	42956.0
30	50487.5
31	58019.0
32	65171.0
33	72323.0
34	80230.0
35	88137.0
36	94143.0
37	100149.0
38	107756.0
39	115363.0
40	127451.5
41	139540.0
42	154141.5
43	168743.0
44	171552.0
45	174361.0
46	178275.0
47	182189.0
48	176563.5
49	170938.0
50	161861.0
51	152784.0
52	136716.5
53	120649.0
54	106540.0
55	92431.0
56	86566.5
57	80702.0
58	73126.0
59	65550.0
60	59073.0
61	52596.0
62	44068.0
63	35540.0
64	28721.5
65	21903.0
66	16975.5
67	12048.0
68	9575.5
69	7103.0
70	5728.5
71	4354.0
72	3612.0
73	2870.0
74	2192.0
75	1231.5
76	949.0
77	687.5
78	426.0
79	319.0
80	212.0
81	131.5
82	51.0
83	44.0
84	37.0
85	26.5
86	16.0
87	12.0
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2223311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.41983315863395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.67035041341866	19.45536664785587
2	6.68097055801988	3.2629637272918206
3	2.426948664958447	1.7779704444856401
4	1.3712850532856515	1.3394620885665631
5	0.8749732069612912	1.0683349866134813
6	0.6203309087885528	0.908902637745614
7	0.5178181819002194	0.8851523525957368
8	0.42989872300039517	0.8398444072623553
9	0.3662214826402474	0.8048760754664162
>10	5.278030777553015	31.24078851122168
>50	1.2971323103640078	22.016220122759783
>100	0.4632568485899027	14.743728381571403
>500	0.002411821117203373	0.3858611734983386
>1k	1.855247013233364E-4	0.0830890445222441
>5k	0.0	0.0
>10k+	1.855247013233364E-4	1.187439398543085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26210	1.1788724114620042	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03539765691799303	0.0
2	0.0	0.0	0.0	0.11082570094782061	0.0
3	0.0	0.0	0.0	0.18733321609077633	0.0
4	0.0	0.0	0.0	0.29662966629499876	0.0
5	0.0	0.0	0.0	0.508700762061628	0.0
6	0.0	0.0	0.0	0.9185849393089855	0.0
7	0.0	0.0	0.0	1.150761184557626	0.0
8	0.0	0.0	0.0	1.6398965326938066	0.0
9	0.0	0.0	0.0	1.8771103098037116	0.0
10	0.0	0.0	0.0	2.1848495329713207	0.0
11	0.0	0.0	0.0	2.4884507835386054	0.0
12	0.0	0.0	0.0	2.730432224731493	0.0
13	0.0	0.0	0.0	2.8583945296002224	0.0
14	0.0	0.0	0.0	2.9290549095470673	0.0
15	0.0	0.0	0.0	2.99764630319375	0.0
16	0.0	0.0	0.0	3.1169728391574547	0.0
17	0.0	0.0	0.0	3.2722367675957167	0.0
18	0.0	0.0	0.0	3.4578608210907067	0.0
19	0.0	0.0	0.0	3.575298282606437	0.0
20	0.0	0.0	0.0	3.698897725059607	0.0
21	0.0	0.0	0.0	3.842512361068694	0.0
22	0.0	0.0	0.0	4.019185799917331	0.0
23	0.0	0.0	0.0	4.207058751564671	0.0
24	0.0	0.0	0.0	4.3493690266453955	0.0
25	0.0	0.0	0.0	4.474947499472633	0.0
26	0.0	0.0	0.0	4.59409412358415	0.0
27	0.0	0.0	0.0	4.721426736970222	0.0
28	0.0	0.0	0.0	4.8569453396308475	0.0
29	0.0	0.0	0.0	5.009105788618866	0.0
30	0.0	0.0	0.0	5.185779227467502	0.0
31	0.0	0.0	0.0	5.345046194616947	0.0
32	0.0	0.0	0.0	5.503953338062017	0.0
33	0.0	0.0	0.0	5.658857442795902	0.0
34	0.0	0.0	0.0	5.827794672000453	0.0
35	0.0	0.0	0.0	6.011889474751845	0.0
36	0.0	0.0	0.0	6.1906318999006436	0.0
37	0.0	0.0	0.0	6.37135335542351	0.0
38	0.0	0.0	0.0	6.571505290982683	0.0
39	0.0	0.0	0.0	6.839214127038457	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGC	25	3.8919858E-5	45.0	20
CGTTTTT	13940	0.0	43.450504	1
TACGGGA	1145	0.0	41.46288	4
CGTTAGG	350	0.0	40.5	2
GTTAGCG	135	0.0	40.000004	1
ACGGGAT	1355	0.0	39.520298	5
ACGGGAC	965	0.0	39.404144	5
TGTCGCG	40	3.4601726E-7	39.375	1
TATAGCG	115	0.0	39.130436	1
CGGGATA	560	0.0	38.973213	6
AGGGATC	3095	0.0	38.67528	6
TTGCGAG	280	0.0	38.571426	1
TAGGGAC	2710	0.0	38.523987	5
CACGGGA	850	0.0	38.117645	4
TATCGCG	65	9.094947E-12	38.07692	1
TAGGGAT	4810	0.0	38.030148	5
CTCACGA	160	0.0	37.96875	24
CGAGGGA	1790	0.0	37.960896	4
TAAGGGA	3420	0.0	37.960526	4
ATAGGGA	3790	0.0	37.816624	4
>>END_MODULE