FastQCFastQC Report
Thu 26 May 2016
SRR1548183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548183_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2524145
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133370.5283769355563963No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC38160.15117990448250793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81850.041.948691
TACGGGA10200.039.926474
GCGATAA2050.039.5121969
GGGCGAT35600.039.0589877
CGGGTAT1450.038.7931066
TAGGGAC22950.038.7254875
CGAGGGT3150.038.5714264
TAGGGCG8750.038.3142855
ACGGGTA2150.037.674425
GGCGATA8250.037.6363648
CGTTCGA301.1403086E-437.50000414
CCGGTTA301.1403086E-437.50000411
ACGGGAT10950.037.191785
CGTAAGG4850.037.1134032
CGGTAGT3100.037.0161312
TAAGACG2250.037.0000041
ACTATCG552.752131E-936.81818411
CGTTAGG3850.036.818182
CTACGGG5400.036.6666683
ACGTAGG3950.036.4556962
Produced by FastQC (version 0.11.5)