##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548183_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2524145
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13010187608081	31.0	31.0	33.0	30.0	34.0
2	31.521537391869327	31.0	31.0	34.0	30.0	34.0
3	31.632912530777748	31.0	31.0	34.0	30.0	34.0
4	35.444085819158566	37.0	35.0	37.0	33.0	37.0
5	35.30475586782851	37.0	35.0	37.0	33.0	37.0
6	35.346762170952935	37.0	35.0	37.0	33.0	37.0
7	35.75758643025658	37.0	35.0	37.0	35.0	37.0
8	35.75131698060135	37.0	35.0	37.0	35.0	37.0
9	37.49299584611819	39.0	37.0	39.0	35.0	39.0
10	36.777928763997316	39.0	37.0	39.0	32.0	39.0
11	36.47345932979286	39.0	35.0	39.0	32.0	39.0
12	35.842600563755255	37.0	35.0	39.0	31.0	39.0
13	35.58606815377088	37.0	35.0	39.0	30.0	39.0
14	36.495556713263305	38.0	35.0	40.0	31.0	41.0
15	36.747766471419034	38.0	35.0	41.0	31.0	41.0
16	36.863803783063176	38.0	35.0	41.0	31.0	41.0
17	36.72875052740631	38.0	35.0	40.0	31.0	41.0
18	36.66197147945146	38.0	35.0	40.0	31.0	41.0
19	36.58709701700972	38.0	35.0	40.0	31.0	41.0
20	36.4743352699627	38.0	35.0	40.0	31.0	41.0
21	36.31981918629873	38.0	35.0	40.0	30.0	41.0
22	36.250425391568236	38.0	35.0	40.0	30.0	41.0
23	36.19329158982546	38.0	34.0	40.0	30.0	41.0
24	36.04235731307037	38.0	34.0	40.0	30.0	41.0
25	35.89303665201484	38.0	34.0	40.0	30.0	41.0
26	35.85736199782501	38.0	34.0	40.0	30.0	41.0
27	35.84163429596953	38.0	34.0	40.0	30.0	41.0
28	35.8581515721165	38.0	34.0	40.0	30.0	41.0
29	35.87331987663149	38.0	34.0	40.0	30.0	41.0
30	35.780776064766485	38.0	34.0	40.0	29.0	41.0
31	35.64307636843367	38.0	34.0	40.0	29.0	41.0
32	35.484021322071435	37.0	34.0	40.0	29.0	41.0
33	35.378200143018724	37.0	34.0	40.0	27.0	41.0
34	35.22891434525354	37.0	34.0	40.0	27.0	41.0
35	35.07255803450277	37.0	34.0	40.0	26.0	41.0
36	34.89460233069019	37.0	34.0	40.0	25.0	41.0
37	34.83217208203174	37.0	33.0	40.0	25.0	41.0
38	34.74611640773411	37.0	33.0	40.0	25.0	41.0
39	34.69900104787958	37.0	33.0	40.0	25.0	41.0
40	34.56938091908349	37.0	33.0	40.0	24.0	41.0
41	34.50882734549719	37.0	33.0	40.0	24.0	41.0
42	34.44401173466659	36.0	33.0	40.0	24.0	41.0
43	34.344205661719116	36.0	33.0	40.0	24.0	41.0
44	34.20591527031926	36.0	33.0	40.0	23.0	41.0
45	34.10186261090389	36.0	33.0	40.0	23.0	41.0
46	34.00028841449283	36.0	33.0	40.0	23.0	41.0
47	33.957393889812195	36.0	33.0	40.0	23.0	41.0
48	33.833533335050085	35.0	33.0	40.0	23.0	41.0
49	33.7227068967908	36.0	33.0	40.0	23.0	41.0
50	33.58379807816112	35.0	32.0	39.0	23.0	41.0
51	33.220747223317204	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	1.0
13	17.0
14	35.0
15	92.0
16	277.0
17	582.0
18	1361.0
19	2704.0
20	4626.0
21	7389.0
22	11248.0
23	16372.0
24	22970.0
25	31101.0
26	41239.0
27	49792.0
28	56983.0
29	65322.0
30	77698.0
31	93519.0
32	115752.0
33	145687.0
34	227107.0
35	289012.0
36	201358.0
37	247872.0
38	341398.0
39	472538.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.747779941326666	23.974177394721778	30.053582500212944	14.22446016373861
2	30.499317590708934	25.83013257954674	30.26755594468622	13.402993885058109
3	28.984586860105104	25.25881040906921	31.668545190549676	14.088057540276013
4	25.205287334919348	28.59356336502063	31.304540745480153	14.896608554579869
5	23.27092936420055	32.85932464260175	29.733949515578544	14.135796477619152
6	22.34780490027316	40.85054543221566	26.573156454958013	10.228493212553163
7	88.86668555094893	3.3027817340129038	6.3214276517395005	1.5091050632986613
8	89.58118491608049	2.44569943485814	5.978024241872	1.995091407189365
9	84.56677409578293	5.019481844347294	7.730261137929874	2.6834829219399046
10	43.81344970277064	30.787018970780206	14.077519318422674	11.32201200802648
11	37.589718498739174	21.798866546890135	24.456637792202905	16.154777162167782
12	33.99824495027029	21.139673037800918	27.55994604113472	17.302135970794073
13	24.243773634240505	29.526235616416646	28.17025170899453	18.059739040348315
14	19.06368295006824	32.51845674475911	30.15528030283522	18.262580002337426
15	17.791252087340464	25.565171572948465	39.66111297092679	16.98246336878428
16	22.25969585740914	22.844289848641814	37.70029059344848	17.195723700500565
17	22.27926684085106	23.127395613168023	28.759679020024603	25.833658525956317
18	24.038119838598814	24.563446236250293	31.95791050038726	19.440523424763633
19	27.446244173769728	26.089269831962902	27.426078929696985	19.03840706457038
20	29.398667667665684	25.883101010441163	26.43037543405787	18.287855887835285
21	25.557525419498482	26.62101424442732	29.44886288228291	18.372597453791283
22	24.536902594740003	23.32029261393462	29.111203991846747	23.031600799478635
23	21.74724510675892	26.8449712674985	28.855751155341707	22.552032470400867
24	21.751404931174715	24.77646886371425	34.64701908963233	18.825107115478705
25	21.164750836421838	25.92687821024545	31.851617082219917	21.056753871112793
26	20.02856412765511	30.958760293089345	27.85359002751427	21.15908555174128
27	19.736861392669596	30.3213563404638	30.15171473905025	19.790067527816348
28	18.383650701524676	27.908737414055057	34.55609721311573	19.15151467130454
29	19.94112858017269	25.89403540604838	33.44807845825022	20.7167575555287
30	21.354676534034297	27.918602140526794	30.991444627784855	19.735276697654058
31	27.112230081869303	25.915349554007395	27.124511468239742	19.847908895883556
32	27.16801134641631	26.31706974044676	28.104249161597295	18.41066975153963
33	26.117081229485628	25.997714077440083	27.63577369762831	20.249430995445984
34	21.15500496207627	26.962634872402337	29.78077725328775	22.101582912233646
35	20.81706875001238	27.283614847799946	30.129172452454196	21.770143949733477
36	25.70850723710405	26.33822541890422	28.6071125074035	19.34615483658823
37	21.965101054020273	29.77118984844373	28.354829060929543	19.908880036606455
38	21.757149450606047	30.346236052207775	25.6246372534066	22.271977243779578
39	21.375871829867144	29.09210841690949	27.872962924079243	21.659056829144127
40	23.668053935094854	25.680973161209042	27.586767004272733	23.064205899423367
41	19.69046944608967	25.298823958211592	28.395119931699647	26.615586663999096
42	21.63489023015714	26.29199194182585	27.328897507868998	24.74422032014801
43	21.474717181461443	25.687232706520426	28.596851607177875	24.241198504840252
44	20.78466173694459	27.45080017193941	29.23611757644668	22.52842051466932
45	20.03474443821571	30.624270792684253	27.112031994992364	22.22895277410767
46	22.39118592632357	28.820610543372112	27.65019442226972	21.138009108034602
47	20.847613746436913	27.4563466044938	28.95859786185025	22.73744178721904
48	22.100632095224324	25.397590075055117	30.932850529585266	21.568927300135293
49	22.115448993619623	24.51634117691337	31.089378779745218	22.278831049721788
50	20.552900090921877	28.591899435254316	28.587264202333863	22.267936271489948
51	19.371311869959925	29.59532831909419	27.703915583296524	23.32944422764936
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1200.0
1	2300.5
2	3401.0
3	13425.0
4	23449.0
5	16748.0
6	10047.0
7	10136.5
8	10226.0
9	10929.0
10	11632.0
11	12242.5
12	12853.0
13	12403.5
14	11954.0
15	11583.5
16	11213.0
17	10620.0
18	10027.0
19	10178.0
20	10329.0
21	10705.0
22	11081.0
23	11882.0
24	12683.0
25	16076.5
26	22152.0
27	24834.0
28	30281.5
29	35729.0
30	39189.5
31	42650.0
32	51134.0
33	59618.0
34	65282.0
35	70946.0
36	76632.5
37	82319.0
38	94840.5
39	107362.0
40	127550.5
41	147739.0
42	170599.5
43	193460.0
44	200652.0
45	207844.0
46	212312.0
47	216780.0
48	210532.5
49	204285.0
50	194844.0
51	185403.0
52	171493.5
53	157584.0
54	142682.5
55	127781.0
56	119086.5
57	110392.0
58	104868.0
59	99344.0
60	91741.0
61	84138.0
62	75307.0
63	66476.0
64	57080.5
65	47685.0
66	40161.5
67	32638.0
68	27493.0
69	22348.0
70	18625.0
71	14902.0
72	12266.5
73	9631.0
74	8197.5
75	5000.5
76	3237.0
77	2269.0
78	1301.0
79	1047.0
80	793.0
81	571.0
82	349.0
83	232.0
84	115.0
85	87.0
86	59.0
87	39.0
88	19.0
89	21.5
90	24.0
91	19.0
92	14.0
93	12.5
94	11.0
95	8.5
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2524145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.68408944480393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.2132558940999	17.610355049114123
2	7.27094432173739	3.153276140414856
3	2.230268285049286	1.4508401093675456
4	1.140223923426961	0.9889887017078196
5	0.6367796633610491	0.6903993588476561
6	0.4603160092462707	0.5988920110422796
7	0.36639321577557044	0.5561432283992771
8	0.27694023732610373	0.48041575016355687
9	0.220264750034371	0.42986164871544114
>10	3.5256025560255213	20.017667553735656
>50	1.7818162019724642	27.578911090205203
>100	0.8731474882082692	25.248965093569915
>500	0.0036795033971731925	0.5109937970347813
>1k	1.8397516985865962E-4	0.1522329869220646
>5k	0.0	0.0
>10k+	1.8397516985865962E-4	0.5320574807598468
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13337	0.5283769355563963	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3816	0.15117990448250793	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9617375388497885E-5	0.0	0.0	0.03890426263150493	0.0
2	3.9617375388497885E-5	0.0	0.0	0.12637942748930825	0.0
3	3.9617375388497885E-5	0.0	0.0	0.20208823185672772	0.0
4	3.9617375388497885E-5	0.0	0.0	0.35342660584078966	0.0
5	3.9617375388497885E-5	0.0	0.0	0.6010352020189015	0.0
6	3.9617375388497885E-5	0.0	0.0	1.032270333122701	0.0
7	3.9617375388497885E-5	0.0	0.0	1.2440648219496107	0.0
8	3.9617375388497885E-5	0.0	0.0	1.781910310223858	0.0
9	3.9617375388497885E-5	0.0	0.0	2.0054711595411514	0.0
10	3.9617375388497885E-5	0.0	0.0	2.3381778780537568	0.0
11	3.9617375388497885E-5	0.0	0.0	2.704876304649693	0.0
12	3.9617375388497885E-5	0.0	0.0	3.005255244845284	0.0
13	3.9617375388497885E-5	0.0	0.0	3.1409447555508896	0.0
14	3.9617375388497885E-5	0.0	0.0	3.2025893916553922	0.0
15	3.9617375388497885E-5	0.0	0.0	3.277703935391984	0.0
16	3.9617375388497885E-5	0.0	0.0	3.4318551430286295	0.0
17	3.9617375388497885E-5	0.0	0.0	3.613580043935669	0.0
18	3.9617375388497885E-5	0.0	0.0	3.825849941267241	0.0
19	3.9617375388497885E-5	0.0	0.0	3.954725263406025	0.0
20	3.9617375388497885E-5	0.0	0.0	4.099447535700207	0.0
21	3.9617375388497885E-5	0.0	0.0	4.275586386677469	0.0
22	3.9617375388497885E-5	0.0	0.0	4.469592673954943	0.0
23	3.9617375388497885E-5	0.0	0.0	4.667798403023598	0.0
24	3.9617375388497885E-5	0.0	0.0	4.824564357435884	0.0
25	3.9617375388497885E-5	0.0	0.0	4.9617989457816405	0.0
26	3.9617375388497885E-5	0.0	0.0	5.090040389914209	0.0
27	3.9617375388497885E-5	0.0	0.0	5.222085102084072	0.0
28	3.9617375388497885E-5	0.0	0.0	5.358685812423612	0.0
29	3.9617375388497885E-5	0.0	0.0	5.518462687365425	0.0
30	3.9617375388497885E-5	0.0	0.0	5.709061880359488	0.0
31	3.9617375388497885E-5	0.0	0.0	5.874781361609575	0.0
32	3.9617375388497885E-5	0.0	0.0	6.040381990733496	0.0
33	3.9617375388497885E-5	0.0	0.0	6.202813229826337	0.0
34	3.9617375388497885E-5	0.0	0.0	6.383191139970168	0.0
35	3.9617375388497885E-5	0.0	0.0	6.581793042792708	0.0
36	3.9617375388497885E-5	0.0	0.0	6.76082396217333	0.0
37	3.9617375388497885E-5	0.0	0.0	6.944767436102126	0.0
38	3.9617375388497885E-5	0.0	0.0	7.157671211439913	0.0
39	3.9617375388497885E-5	0.0	0.0	7.438003759688924	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8185	0.0	41.94869	1
TACGGGA	1020	0.0	39.92647	4
GCGATAA	205	0.0	39.512196	9
GGGCGAT	3560	0.0	39.058987	7
CGGGTAT	145	0.0	38.793106	6
TAGGGAC	2295	0.0	38.725487	5
CGAGGGT	315	0.0	38.571426	4
TAGGGCG	875	0.0	38.314285	5
ACGGGTA	215	0.0	37.67442	5
GGCGATA	825	0.0	37.636364	8
CGTTCGA	30	1.1403086E-4	37.500004	14
CCGGTTA	30	1.1403086E-4	37.500004	11
ACGGGAT	1095	0.0	37.19178	5
CGTAAGG	485	0.0	37.113403	2
CGGTAGT	310	0.0	37.01613	12
TAAGACG	225	0.0	37.000004	1
ACTATCG	55	2.752131E-9	36.818184	11
CGTTAGG	385	0.0	36.81818	2
CTACGGG	540	0.0	36.666668	3
ACGTAGG	395	0.0	36.455696	2
>>END_MODULE