FastQCFastQC Report
Thu 26 May 2016
SRR1548182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548182_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2023172
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167230.8265733214971341No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25520.1261385586593725No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG551.8189894E-1245.01
AACGCGT207.034561E-445.026
GTATCGC207.034561E-445.024
TATGTCG253.891862E-545.026
CGTTTTT95000.042.6789471
ACGGGAT9200.042.065225
CGACGGT3400.041.69117728
ACGTTAG603.6379788E-1241.2500041
GGGCGAT27600.040.5163047
AATCGGT1000.040.526
AGGGCGA15750.040.1428576
CACGACG3500.039.85714326
TCACGAC3700.039.52702725
GGCGATA7600.039.3750048
ACTACGT403.4599543E-739.37528
CGGTCTA3550.039.29577331
GCGAGAC3750.039.021
ACACGAC3750.039.026
TACGGGA8350.038.8023954
TCCAACG356.250644E-638.5714314
Produced by FastQC (version 0.11.5)