##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548182_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2023172
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09026667035724	31.0	31.0	33.0	30.0	34.0
2	31.458489935606067	31.0	31.0	34.0	30.0	34.0
3	31.52957632865619	31.0	31.0	34.0	30.0	34.0
4	35.36369522709883	37.0	35.0	37.0	33.0	37.0
5	35.21840209334649	37.0	35.0	37.0	32.0	37.0
6	35.268987016427666	37.0	35.0	37.0	32.0	37.0
7	35.73091165753579	37.0	35.0	37.0	35.0	37.0
8	35.72436896121536	37.0	35.0	37.0	35.0	37.0
9	37.44585136607268	39.0	37.0	39.0	35.0	39.0
10	36.6960149705512	39.0	37.0	39.0	32.0	39.0
11	36.42391897475845	38.0	35.0	39.0	32.0	39.0
12	35.88125181645456	37.0	35.0	39.0	31.0	39.0
13	35.661351086313964	37.0	35.0	39.0	30.0	39.0
14	36.58415102621033	38.0	35.0	41.0	31.0	41.0
15	36.83628480425787	38.0	35.0	41.0	31.0	41.0
16	36.931513979038854	38.0	35.0	41.0	31.0	41.0
17	36.79102715933198	38.0	35.0	41.0	31.0	41.0
18	36.72465069702427	38.0	35.0	40.0	31.0	41.0
19	36.637134163580754	38.0	35.0	40.0	31.0	41.0
20	36.476467645855124	38.0	35.0	40.0	31.0	41.0
21	36.33037477782413	38.0	35.0	40.0	30.0	41.0
22	36.25655307606076	38.0	35.0	40.0	30.0	41.0
23	36.194393753966544	38.0	34.0	40.0	30.0	41.0
24	36.01998693141265	38.0	34.0	40.0	30.0	41.0
25	35.90918023776525	38.0	34.0	40.0	30.0	41.0
26	35.818323899302676	38.0	34.0	40.0	30.0	41.0
27	35.77882404461904	38.0	34.0	40.0	30.0	41.0
28	35.790846749559606	38.0	34.0	40.0	29.0	41.0
29	35.79139489870362	38.0	34.0	40.0	29.0	41.0
30	35.70817706057616	38.0	34.0	40.0	29.0	41.0
31	35.55989011314905	38.0	34.0	40.0	29.0	41.0
32	35.37946254693126	37.0	34.0	40.0	27.0	41.0
33	35.22828805459941	37.0	34.0	40.0	27.0	41.0
34	35.02894563586289	37.0	34.0	40.0	26.0	41.0
35	34.86365271959082	37.0	34.0	40.0	25.0	41.0
36	34.64506527373847	37.0	33.0	40.0	24.0	41.0
37	34.560013681486296	37.0	33.0	40.0	24.0	41.0
38	34.46898533589828	37.0	33.0	40.0	23.0	41.0
39	34.42657124554907	37.0	33.0	40.0	23.0	41.0
40	34.29258510892796	37.0	33.0	40.0	23.0	41.0
41	34.21084218247386	37.0	33.0	40.0	23.0	41.0
42	34.157352909194074	37.0	33.0	40.0	23.0	41.0
43	34.0641057705425	36.0	33.0	40.0	23.0	41.0
44	33.92724246875698	36.0	33.0	40.0	23.0	41.0
45	33.83680329700095	36.0	33.0	40.0	23.0	41.0
46	33.72408623685974	36.0	33.0	40.0	23.0	41.0
47	33.667301148888974	36.0	33.0	40.0	23.0	41.0
48	33.540157732511126	35.0	32.0	40.0	22.0	41.0
49	33.428189990766974	36.0	32.0	40.0	21.0	41.0
50	33.27628595097204	35.0	32.0	39.0	20.0	41.0
51	32.92420120484071	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	6.0
13	6.0
14	28.0
15	88.0
16	250.0
17	610.0
18	1308.0
19	2665.0
20	4448.0
21	6928.0
22	10585.0
23	15026.0
24	20564.0
25	28722.0
26	37754.0
27	43525.0
28	48028.0
29	53797.0
30	62706.0
31	74962.0
32	91684.0
33	114550.0
34	171278.0
35	221744.0
36	164469.0
37	197029.0
38	270227.0
39	380111.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.570170010261116	23.294312100009293	27.72240817883996	14.413109710889632
2	32.48369392221719	25.063365843339074	28.65480542435344	13.798134810090293
3	28.76285357844019	25.209917891311267	32.13928425264881	13.887944277599729
4	25.77877708865089	28.094546583286046	31.414284104366807	14.712392223696256
5	23.24345137239938	31.446609581390017	30.869891437801627	14.440047608408973
6	21.12692346473755	40.736427748110394	27.808461168897157	10.328187618254898
7	86.83750071669635	3.7553406235357154	7.769482772596695	1.637675887171234
8	87.33300974904753	3.361009345720482	7.380094228271249	1.9258866769607328
9	82.77793484686423	5.756060285531828	9.031758051218581	2.4342468163853592
10	40.488697945602254	33.0946652088898	15.345408101733318	11.071228743774627
11	34.74627960450224	24.327491681379538	25.5554643895823	15.370764324535926
12	32.17670074516651	23.28160927494054	27.919524390412683	16.622165589480282
13	22.446682733845662	32.579582951919065	28.37964345097698	16.59409086325829
14	17.32220493363886	34.776331424120144	30.73693190692635	17.164531735314643
15	16.985604782984343	27.427623553509044	39.37964740516377	16.20712425834284
16	19.902064678633355	25.38177673475117	38.00368925627678	16.712469330338696
17	20.097549788154442	25.05530918775072	29.50525214860625	25.34188887548859
18	21.85370299707588	27.03877870986748	32.41167829527099	18.695839997785654
19	25.815205034470623	27.843159157995462	27.654495020690284	18.687140786843628
20	26.90754913571362	27.478830272463238	28.132655058492308	17.480965533330828
21	23.19812650629803	28.736508808939625	30.459990549493565	17.605374135268775
22	23.139258550434665	24.857797557498817	29.64300613096662	22.359937761099896
23	19.681470482984146	30.11775568266069	28.91420007789748	21.28657375645768
24	19.22916094133371	27.279588685489912	35.41157153222761	18.07967884094877
25	20.002006749796852	28.306392140658332	32.42937328116443	19.262227828380386
26	18.877732590209828	33.46971982609486	27.961191633731588	19.69135594996372
27	18.78016303112143	32.96595642881574	30.160905746026533	18.092974794036294
28	17.216232727617818	30.466762094374577	34.08751208498339	18.22949309302422
29	17.97454689962099	27.797043454535746	34.03976527947204	20.18864436637122
30	19.452127649058014	29.925384495238173	31.749352007639487	18.873135848064326
31	24.741198474474736	28.97820847659023	27.52771390667724	18.7528791422578
32	25.25247482665834	28.444146123018704	28.25281290962904	18.05056614069392
33	24.466085928433174	29.479549934459353	26.937996374010716	19.11636776309676
34	19.49399260171651	30.558400373275234	28.92087276810869	21.026734256899562
35	20.567850879707706	29.276947288712975	29.412131049658658	20.743070781920668
36	25.173045099477452	29.057687631106006	27.58292423975816	18.186343029658378
37	19.72738847710427	32.23556870102986	29.05714393042213	18.97989889144373
38	20.237873991929504	32.45660774269316	26.928901744389506	20.376616520987834
39	19.448766590284958	31.48382836456811	28.458727186813576	20.608677858333348
40	22.449697801274436	28.544533040196285	28.047590615133068	20.958178543396212
41	18.657039539890825	27.73036597975852	29.590810865314467	24.021783615036192
42	21.390816005757294	28.279058824459806	27.703477509574075	22.62664766020882
43	21.092423184978834	28.20867429956524	28.122917873517427	22.5759846419385
44	19.716860454771023	30.478871791424556	28.820090432251927	20.984177321552494
45	19.15561306700567	33.23138121721732	26.20073824667403	21.41226746910297
46	21.480674900601628	31.73674803724053	26.47738304009743	20.30519402206041
47	20.18889150304571	29.92024405240879	28.284990104647555	21.605874339897944
48	21.057873477885224	29.539011018341494	29.52235400648091	19.880761497292372
49	21.003651691502252	28.124894966913345	29.481329318515677	21.390124023068726
50	19.36740919704306	31.671849946519625	28.71485963625436	20.24588122018296
51	18.583096246883606	31.96614029850156	27.12720421199977	22.32355924261506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1244.0
1	2342.5
2	3441.0
3	14065.0
4	24689.0
5	17468.5
6	10248.0
7	10549.5
8	10851.0
9	12059.0
10	13267.0
11	14268.5
12	15270.0
13	15586.0
14	15902.0
15	15096.5
16	14291.0
17	13325.5
18	12360.0
19	12318.0
20	12276.0
21	12869.5
22	13463.0
23	14194.5
24	14926.0
25	16830.0
26	21758.5
27	24783.0
28	29125.0
29	33467.0
30	38024.5
31	42582.0
32	51074.5
33	59567.0
34	64842.5
35	70118.0
36	76340.5
37	82563.0
38	89537.0
39	96511.0
40	113884.0
41	131257.0
42	147533.5
43	163810.0
44	164991.5
45	166173.0
46	165878.5
47	165584.0
48	162280.5
49	158977.0
50	150645.0
51	142313.0
52	127563.0
53	112813.0
54	102081.5
55	91350.0
56	84313.0
57	77276.0
58	69706.5
59	62137.0
60	54689.5
61	47242.0
62	42188.5
63	37135.0
64	31247.5
65	25360.0
66	20770.0
67	16180.0
68	14094.0
69	12008.0
70	10354.0
71	8700.0
72	7431.5
73	6163.0
74	5187.0
75	3086.5
76	1962.0
77	1414.0
78	866.0
79	739.5
80	613.0
81	468.0
82	323.0
83	205.0
84	87.0
85	62.0
86	37.0
87	29.5
88	22.0
89	18.0
90	14.0
91	9.5
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2023172.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.729931119925542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.23728569500473	19.27755193912175
2	7.108693741464673	3.37377625675205
3	2.4251123627837075	1.7264324798081179
4	1.2244430362275907	1.1622379563981289
5	0.8205143596319359	0.9735374618487825
6	0.5404512804353903	0.7694922995044426
7	0.43080022354876574	0.7156001741882515
8	0.31499206240898653	0.5979791955430832
9	0.2720080238531931	0.5809258503093004
>10	3.5133878682223867	20.60186134074991
>50	1.3723179193641903	23.19977406527198
>100	0.7364266882848575	25.53398208137954
>500	0.0027275061179671796	0.41741699605751653
>1k	6.294244887616568E-4	0.23683834091669922
>5k	0.0	0.0
>10k+	2.0980816292055223E-4	0.832593562150507
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16723	0.8265733214971341	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2552	0.1261385586593725	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.885466979574648E-5	0.0	0.0	0.03341287839096231	0.0
2	9.885466979574648E-5	0.0	0.0	0.10078233585676354	0.0
3	9.885466979574648E-5	0.0	0.0	0.15544896825381135	0.0
4	9.885466979574648E-5	0.0	0.0	0.27446999068789013	0.0
5	9.885466979574648E-5	0.0	0.0	0.4686699895016341	0.0
6	9.885466979574648E-5	0.0	0.0	0.8770386304278628	0.0
7	9.885466979574648E-5	0.0	0.0	1.092195819238305	0.0
8	9.885466979574648E-5	0.0	0.0	1.5824161267554118	0.0
9	9.885466979574648E-5	0.0	0.0	1.7909994800244369	0.0
10	9.885466979574648E-5	0.0	0.0	2.098734067098596	0.0
11	9.885466979574648E-5	0.0	0.0	2.4029098860601077	0.0
12	9.885466979574648E-5	0.0	0.0	2.6500959878843715	0.0
13	9.885466979574648E-5	0.0	0.0	2.774652871827012	0.0
14	9.885466979574648E-5	0.0	0.0	2.841429201274039	0.0
15	1.4828200469361972E-4	0.0	0.0	2.908600949400249	0.0
16	1.4828200469361972E-4	0.0	0.0	3.0357280547575787	0.0
17	1.4828200469361972E-4	0.0	0.0	3.191523014355675	0.0
18	1.4828200469361972E-4	0.0	0.0	3.376331819538823	0.0
19	1.4828200469361972E-4	0.0	0.0	3.48215574355517	0.0
20	1.4828200469361972E-4	0.0	0.0	3.595591477145789	0.0
21	1.4828200469361972E-4	0.0	0.0	3.7469379766030766	0.0
22	1.9770933959149296E-4	0.0	0.0	3.910591882449935	0.0
23	1.9770933959149296E-4	0.0	0.0	4.092286765534517	0.0
24	1.9770933959149296E-4	0.0	0.0	4.233253524663252	0.0
25	1.9770933959149296E-4	0.0	0.0	4.355783887875079	0.0
26	1.9770933959149296E-4	0.0	0.0	4.474903764978954	0.0
27	1.9770933959149296E-4	0.0	0.0	4.598472102223637	0.0
28	1.9770933959149296E-4	0.0	0.0	4.734446700527686	0.0
29	1.9770933959149296E-4	0.0	0.0	4.880702184490493	0.0
30	1.9770933959149296E-4	0.0	0.0	5.066746673046088	0.0
31	1.9770933959149296E-4	0.0	0.0	5.234552475024367	0.0
32	1.9770933959149296E-4	0.0	0.0	5.386838093844715	0.0
33	1.9770933959149296E-4	0.0	0.0	5.539617986014041	0.0
34	1.9770933959149296E-4	0.0	0.0	5.706138677284977	0.0
35	1.9770933959149296E-4	0.0	0.0	5.89895471072158	0.0
36	1.9770933959149296E-4	0.0	0.0	6.061372933195991	0.0
37	2.4713667448936623E-4	0.0	0.0	6.234813451352628	0.0
38	2.4713667448936623E-4	0.0	0.0	6.4306939795529	0.0
39	2.4713667448936623E-4	0.0	0.0	6.736500900566042	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	55	1.8189894E-12	45.0	1
AACGCGT	20	7.034561E-4	45.0	26
GTATCGC	20	7.034561E-4	45.0	24
TATGTCG	25	3.891862E-5	45.0	26
CGTTTTT	9500	0.0	42.678947	1
ACGGGAT	920	0.0	42.06522	5
CGACGGT	340	0.0	41.691177	28
ACGTTAG	60	3.6379788E-12	41.250004	1
GGGCGAT	2760	0.0	40.516304	7
AATCGGT	100	0.0	40.5	26
AGGGCGA	1575	0.0	40.142857	6
CACGACG	350	0.0	39.857143	26
TCACGAC	370	0.0	39.527027	25
GGCGATA	760	0.0	39.375004	8
ACTACGT	40	3.4599543E-7	39.375	28
CGGTCTA	355	0.0	39.295773	31
GCGAGAC	375	0.0	39.0	21
ACACGAC	375	0.0	39.0	26
TACGGGA	835	0.0	38.802395	4
TCCAACG	35	6.250644E-6	38.57143	14
>>END_MODULE