FastQCFastQC Report
Thu 26 May 2016
SRR1548181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548181_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1415015
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111320.7867054412850747No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48060.33964304265325806No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC351.2121927E-745.00000426
CCGGGAT453.8562575E-1045.0000045
CTATACG700.045.0000041
GTCTCCG351.2121927E-745.00000430
CACTGCG453.8562575E-1045.0000041
TAACACG700.045.0000041
TGCGAAG900.045.0000041
ACAGCCG351.2121927E-745.00000434
CGACTAT351.2121927E-745.00000420
TCCGGAT207.0338405E-445.018
CGGGTAG750.045.06
AGTCCGA207.0338405E-445.029
CACGGCG207.0338405E-445.022
ACGTTAC207.0338405E-445.041
CAACCGT253.8912644E-545.037
CGCGCCT207.0338405E-445.017
CAATTCG207.0338405E-445.01
ACTTACG207.0338405E-445.01
TACGATC502.1827873E-1145.017
TTCCACG551.8189894E-1245.024