FastQCFastQC Report
Thu 26 May 2016
SRR1548180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548180_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1485553
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122610.825349213390569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT502.1827873E-1145.0000044
ACGTATA207.033953E-445.00000439
CGTTTTT72000.041.031251
CGACGGT2150.038.72093228
TAGGGAC17100.038.289475
AAGGGAT23650.038.0549665
AACGGGA5500.037.6363644
CCCCGTA301.14008246E-437.50000438
ACGGTCT2100.037.50000430
TAAGGGA19150.037.4804154
CGGGATA3250.037.3846176
ATAGGGA21700.037.2235034
GCGTAAG1150.037.1739121
ACGGGAA5550.036.891895
GTAGGGA22250.036.808994
CGGTCTA2150.036.62790731
GCGGGAT7200.036.56255
TAGGGAT24400.036.5163965
GGGCGAT19050.036.496067
ACGTAGG2100.036.4285742
Produced by FastQC (version 0.11.5)