##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548180_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1485553
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.084493114685237	31.0	31.0	33.0	30.0	34.0
2	31.476911291620024	31.0	31.0	34.0	30.0	34.0
3	31.5516921981242	31.0	31.0	34.0	30.0	34.0
4	35.381562286905954	37.0	35.0	37.0	33.0	37.0
5	35.234808182542125	37.0	35.0	37.0	33.0	37.0
6	35.283159873797835	37.0	35.0	37.0	32.0	37.0
7	35.71120855331314	37.0	35.0	37.0	35.0	37.0
8	35.707111762421135	37.0	35.0	37.0	35.0	37.0
9	37.41640453083801	39.0	37.0	39.0	35.0	39.0
10	36.743027680601095	39.0	37.0	39.0	32.0	39.0
11	36.44007988944185	39.0	35.0	39.0	32.0	39.0
12	35.84844229724554	37.0	35.0	39.0	31.0	39.0
13	35.57379979038109	37.0	35.0	39.0	30.0	39.0
14	36.4770230345198	38.0	35.0	40.0	30.0	41.0
15	36.72377155173864	38.0	35.0	41.0	31.0	41.0
16	36.81757298460573	38.0	35.0	41.0	31.0	41.0
17	36.68372653146673	38.0	35.0	40.0	31.0	41.0
18	36.6109219933587	38.0	35.0	40.0	31.0	41.0
19	36.5243575961275	38.0	35.0	40.0	31.0	41.0
20	36.36537572203752	38.0	35.0	40.0	30.0	41.0
21	36.22413067726295	38.0	34.0	40.0	30.0	41.0
22	36.143910718769376	38.0	34.0	40.0	30.0	41.0
23	36.06507139092311	38.0	34.0	40.0	30.0	41.0
24	35.89104259491247	38.0	34.0	40.0	30.0	41.0
25	35.75882651107029	37.0	34.0	40.0	29.0	41.0
26	35.696219522292374	37.0	34.0	40.0	29.0	41.0
27	35.67519637468337	37.0	34.0	40.0	29.0	41.0
28	35.67618859778143	37.0	34.0	40.0	29.0	41.0
29	35.677693761178496	37.0	34.0	40.0	29.0	41.0
30	35.604195878571815	37.0	34.0	40.0	29.0	41.0
31	35.437786467396315	37.0	34.0	40.0	28.0	41.0
32	35.25673133169937	37.0	34.0	40.0	27.0	41.0
33	35.093516017267646	37.0	34.0	40.0	26.0	41.0
34	34.88941559136564	37.0	34.0	40.0	25.0	41.0
35	34.69707105704071	37.0	33.0	40.0	24.0	41.0
36	34.51577089474425	37.0	33.0	40.0	24.0	41.0
37	34.43085706130983	37.0	33.0	40.0	23.0	41.0
38	34.35000501496749	37.0	33.0	40.0	23.0	41.0
39	34.294505817025716	37.0	33.0	40.0	23.0	41.0
40	34.14600219581529	37.0	33.0	40.0	23.0	41.0
41	34.07970297929458	36.0	33.0	40.0	23.0	41.0
42	34.03659378022864	36.0	33.0	40.0	23.0	41.0
43	33.91636851731308	36.0	33.0	40.0	23.0	41.0
44	33.76094895301615	36.0	33.0	40.0	22.0	41.0
45	33.66853892119635	36.0	32.0	40.0	23.0	41.0
46	33.549797954027895	35.0	32.0	40.0	22.0	41.0
47	33.504492939666235	35.0	32.0	40.0	22.0	41.0
48	33.38247709775417	35.0	32.0	40.0	22.0	41.0
49	33.26437764253446	35.0	32.0	39.0	21.0	41.0
50	33.125238884105784	35.0	32.0	39.0	20.0	41.0
51	32.78295220702324	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	2.0
13	15.0
14	24.0
15	84.0
16	221.0
17	537.0
18	1097.0
19	2112.0
20	3512.0
21	5409.0
22	7877.0
23	11387.0
24	15666.0
25	21439.0
26	28429.0
27	33726.0
28	36679.0
29	40887.0
30	47127.0
31	56197.0
32	68414.0
33	85079.0
34	129003.0
35	161708.0
36	119140.0
37	145951.0
38	199456.0
39	264320.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.102998681299155	25.04851728615539	29.27973623290451	15.568747799640942
2	31.85110191289035	25.235720300790344	29.408442512653536	13.504735273665766
3	29.09939934825617	24.84946683154354	32.4525614367175	13.598572383482784
4	26.261735528789615	27.676023676031754	31.73289677312085	14.32934402205778
5	23.338043139490814	31.52381638352856	30.976074229596655	14.16206624738397
6	22.191197486727166	40.56240336090332	27.26520023183286	9.981198920536661
7	86.94587133545555	3.654329397874058	7.88029777463342	1.519501492036972
8	87.82392819374334	2.905382709334504	7.5193547453372584	1.7513343515848978
9	82.41173488929712	5.748229783790952	9.103074747249003	2.736960579662927
10	42.30424629750672	34.0941050235165	14.019627707661725	9.58202097131506
11	36.60232923362546	21.861017412371016	25.956327374385165	15.580325979618365
12	34.45417295781436	20.216377335578066	28.60759595921519	16.721853747392384
13	24.998165666253577	28.636002889159794	28.816743663807348	17.549087780779278
14	19.14250114267212	31.010270249529974	30.950292584646927	18.896936023150975
15	18.696337323542142	24.650147116932214	39.713022692559605	16.94049286696604
16	22.97986002518927	22.53470593105732	37.193624192472434	17.29180985128097
17	22.20728577169579	22.860981735421085	29.232615732996397	25.69911675988672
18	23.942801098311538	24.363721792490743	32.2583576620962	19.435119447101517
19	27.097518567159838	25.37021567052808	27.85831269567629	19.67395306663579
20	28.54566615933595	25.658593129965745	27.809643950771196	17.98609675992711
21	25.69662610489158	25.650448014981624	30.173746746161196	18.4791791339656
22	24.75421610672928	23.102306009950503	29.89075448671303	22.252723396607188
23	22.26019536159262	26.097890819109114	29.82330485684456	21.818608962453713
24	22.496807586131226	24.04135025811937	34.031367443638835	19.430474712110573
25	21.7477935825918	25.565833060146627	31.601699838376685	21.084673518884887
26	20.433737470154213	29.93491312662692	28.104618280195993	21.526731123022874
27	19.632823601715994	29.03767149337654	31.384945538799357	19.94455936610811
28	18.92116942310372	27.062582082227966	34.10608709349313	19.910161401175188
29	19.948127061101154	25.289639615685207	33.89774716889939	20.864486154314253
30	21.531577803013423	26.824219667692773	31.6260005533293	20.018201975964505
31	26.166888693974567	25.901936854491225	28.249480159913514	19.681694291620698
32	27.200914406958216	25.063124641126905	28.45452164951368	19.281439302401193
33	26.434869708452002	25.979079844340795	27.77033199084785	19.81571845635935
34	21.341076353384903	26.166821378974696	30.098219316308473	22.39388295133193
35	21.595527052888723	26.562835523202473	30.923299269699566	20.91833815420924
36	26.193410803922852	26.499155533326647	27.618738611143463	19.688695051607045
37	21.511921823051754	29.496490532481843	29.050394028351732	19.94119361611467
38	21.94765181720208	29.77719408193447	26.725805137884677	21.54934896297877
39	21.585227857908805	28.921418488603233	28.071230040261103	21.42212361322686
40	24.131013837944522	25.67636428993109	27.78891093081162	22.403710941312763
41	19.976130101046547	25.06622113112087	28.69140313405176	26.266245633780823
42	21.974577817149573	25.658054609966797	27.461423456450223	24.90594411643341
43	21.98157857713592	26.016439669267942	28.038851525324237	23.963130228271893
44	21.19742614366502	27.540922471295204	28.571582434285414	22.690068950754366
45	20.498427185028067	30.010171296480166	26.62792912807554	22.863472390416227
46	22.254338956604037	29.002533063445064	27.395050866579652	21.34807711337125
47	21.684248222715716	26.96369634742079	28.683459964067254	22.66859546579624
48	22.233875196643943	25.650515329981495	30.60139894032727	21.51421053304729
49	22.14777931181183	24.924051851398097	30.40133876071739	22.52683007607268
50	20.147312145712743	28.422075819576953	29.38097799270709	22.049634042003213
51	19.672674081638284	28.946325038554665	28.37953273965991	23.00146814014714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	914.0
1	1542.0
2	2170.0
3	11047.5
4	19925.0
5	13946.0
6	7967.0
7	7895.0
8	7823.0
9	8310.5
10	8798.0
11	9043.0
12	9288.0
13	8980.0
14	8672.0
15	8270.5
16	7869.0
17	7483.5
18	7098.0
19	6884.0
20	6670.0
21	6866.5
22	7063.0
23	7378.5
24	7694.0
25	9378.5
26	12563.5
27	14064.0
28	16330.5
29	18597.0
30	21494.0
31	24391.0
32	28421.5
33	32452.0
34	37239.5
35	42027.0
36	45504.0
37	48981.0
38	53337.5
39	57694.0
40	71807.0
41	85920.0
42	96735.5
43	107551.0
44	111908.5
45	116266.0
46	116305.5
47	116345.0
48	111377.5
49	106410.0
50	104065.5
51	101721.0
52	96728.0
53	91735.0
54	84742.5
55	77750.0
56	75593.5
57	73437.0
58	69058.0
59	64679.0
60	60002.5
61	55326.0
62	49122.0
63	42918.0
64	37706.0
65	32494.0
66	27191.0
67	21888.0
68	18494.5
69	15101.0
70	12957.0
71	10813.0
72	8453.0
73	6093.0
74	5047.5
75	3140.0
76	2278.0
77	1539.5
78	801.0
79	603.0
80	405.0
81	286.0
82	167.0
83	132.0
84	97.0
85	79.0
86	61.0
87	44.0
88	27.0
89	25.5
90	24.0
91	20.5
92	17.0
93	11.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1485553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.027208290139047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5119718614513	20.14989889626356
2	5.437288893930852	2.7216032336413436
3	1.904337059356073	1.42980720717406
4	1.0714603336070658	1.0726264377522363
5	0.695746423680745	0.8706295331288669
6	0.5519242155011063	0.8287873381030666
7	0.47434973596900365	0.83101547511281
8	0.4060992219823165	0.813082385201188
9	0.3858276177119161	0.869056933730784
>10	7.120037437056692	44.197901760330396
>50	1.3643375742715809	22.489282805244752
>100	0.075270688414635	2.674747498038141
>500	8.093622400966663E-4	0.12531759863191705
>1k	2.6978741336555545E-4	0.09837701573637023
>5k	0.0	0.0
>10k+	2.6978741336555545E-4	0.8278658819105252
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12261	0.825349213390569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.731499986873575E-5	0.0	0.0	0.02201200495707659	0.0
2	6.731499986873575E-5	0.0	0.0	0.07640252485101508	0.0
3	6.731499986873575E-5	0.0	0.0	0.12338839475939263	0.0
4	6.731499986873575E-5	0.0	0.0	0.1931940496232716	0.0
5	6.731499986873575E-5	0.0	0.0	0.3406138993358029	0.0
6	6.731499986873575E-5	0.0	0.0	0.5708311988868792	0.0
7	1.346299997374715E-4	0.0	0.0	0.707682593620019	0.0
8	1.346299997374715E-4	0.0	0.0	1.013360008023948	0.0
9	1.346299997374715E-4	0.0	0.0	1.1584911477409423	0.0
10	1.346299997374715E-4	0.0	0.0	1.3649462523383549	0.0
11	1.346299997374715E-4	0.0	0.0	1.580152306918703	0.0
12	1.346299997374715E-4	0.0	0.0	1.7492475865889672	0.0
13	1.346299997374715E-4	0.0	0.0	1.8253135364406385	0.0
14	1.346299997374715E-4	0.0	0.0	1.8676546713580733	0.0
15	1.346299997374715E-4	0.0	0.0	1.9149771162657947	0.0
16	1.346299997374715E-4	0.0	0.0	2.0105644160793994	0.0
17	1.346299997374715E-4	0.0	0.0	2.126009640854281	0.0
18	1.346299997374715E-4	0.0	0.0	2.2588221355952967	0.0
19	1.346299997374715E-4	0.0	0.0	2.338792355439355	0.0
20	1.346299997374715E-4	0.0	0.0	2.4298695502617544	0.0
21	1.346299997374715E-4	0.0	0.0	2.544103105038999	0.0
22	1.346299997374715E-4	0.0	0.0	2.667626129798129	0.0
23	1.346299997374715E-4	0.0	0.0	2.800034734539932	0.0
24	1.346299997374715E-4	0.0	0.0	2.9033632593384415	0.0
25	1.346299997374715E-4	0.0	0.0	2.9941038791614973	0.0016828749967183938
26	1.346299997374715E-4	0.0	0.0	3.0853157039836345	0.0016828749967183938
27	1.346299997374715E-4	0.0	0.0	3.1818454137954015	0.0016828749967183938
28	1.346299997374715E-4	0.0	0.0	3.2810004086020492	0.0016828749967183938
29	1.346299997374715E-4	0.0	0.0	3.395233963379294	0.0016828749967183938
30	1.346299997374715E-4	0.0	0.0	3.542653813091825	0.0016828749967183938
31	1.346299997374715E-4	0.0	0.0	3.663416922856337	0.0016828749967183938
32	1.346299997374715E-4	0.0	0.0	3.7866033726161232	0.0016828749967183938
33	1.346299997374715E-4	0.0	0.0	3.9116073273723657	0.0016828749967183938
34	1.346299997374715E-4	0.0	0.0	4.040919442120207	0.0016828749967183938
35	1.346299997374715E-4	0.0	0.0	4.197090241815674	0.0016828749967183938
36	1.346299997374715E-4	0.0	0.0	4.339999986537	0.0016828749967183938
37	1.346299997374715E-4	0.0	0.0	4.482505841259114	0.0016828749967183938
38	1.346299997374715E-4	0.0	0.0	4.649245095933972	0.0016828749967183938
39	1.346299997374715E-4	0.0	0.0	4.901878290441338	0.0016828749967183938
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	50	2.1827873E-11	45.000004	4
ACGTATA	20	7.033953E-4	45.000004	39
CGTTTTT	7200	0.0	41.03125	1
CGACGGT	215	0.0	38.720932	28
TAGGGAC	1710	0.0	38.28947	5
AAGGGAT	2365	0.0	38.054966	5
AACGGGA	550	0.0	37.636364	4
CCCCGTA	30	1.14008246E-4	37.500004	38
ACGGTCT	210	0.0	37.500004	30
TAAGGGA	1915	0.0	37.480415	4
CGGGATA	325	0.0	37.384617	6
ATAGGGA	2170	0.0	37.223503	4
GCGTAAG	115	0.0	37.173912	1
ACGGGAA	555	0.0	36.89189	5
GTAGGGA	2225	0.0	36.80899	4
CGGTCTA	215	0.0	36.627907	31
GCGGGAT	720	0.0	36.5625	5
TAGGGAT	2440	0.0	36.516396	5
GGGCGAT	1905	0.0	36.49606	7
ACGTAGG	210	0.0	36.428574	2
>>END_MODULE