FastQCFastQC Report
Thu 26 May 2016
SRR1548179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548179_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2427637
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117990.48602818296145595No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31450.1295498462084735No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76850.041.3109971
TCGTAAT501.0822987E-940.528
TCACGAC2350.040.21276525
GGGCGAT38100.039.9803167
CGACCCG2100.039.64285332
GCGATAA2050.039.512199
ACGGGAT8250.039.2727285
GCGATAT2750.039.2727289
CGAAAGG4750.038.8421062
TACGGGA9950.038.6683434
GGCGATA8900.038.1741568
CTTAACG659.094947E-1238.076921
TACGCAA301.1402959E-437.49999628
CGCTTAA301.1402959E-437.49999640
AGGGCGA19850.037.2921946
ACCGTTC850.037.05882643
TAAGGGA32350.036.931994
CGAACGG1950.036.9230772
CACGACG2500.036.89999826
TATCGGA552.752131E-936.81818437
Produced by FastQC (version 0.11.5)