##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548179_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2427637
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077547425747753	31.0	31.0	33.0	30.0	34.0
2	31.461698763035823	31.0	31.0	34.0	30.0	34.0
3	31.58036395062359	31.0	31.0	34.0	30.0	34.0
4	35.387464435580775	37.0	35.0	37.0	33.0	37.0
5	35.23705273893914	37.0	35.0	37.0	33.0	37.0
6	35.27568413234763	37.0	35.0	37.0	32.0	37.0
7	35.717012469327166	37.0	35.0	37.0	35.0	37.0
8	35.7058991933308	37.0	35.0	37.0	35.0	37.0
9	37.41534298579236	39.0	37.0	39.0	35.0	39.0
10	36.6901385997989	39.0	37.0	39.0	32.0	39.0
11	36.3814940207288	38.0	35.0	39.0	32.0	39.0
12	35.738321668354864	37.0	35.0	39.0	31.0	39.0
13	35.4477110869541	37.0	35.0	39.0	30.0	39.0
14	36.3645804541618	38.0	35.0	40.0	30.0	41.0
15	36.64791070493653	38.0	35.0	40.0	31.0	41.0
16	36.765519309517856	38.0	35.0	40.0	31.0	41.0
17	36.62841973491094	38.0	35.0	40.0	31.0	41.0
18	36.568020260030636	38.0	35.0	40.0	31.0	41.0
19	36.4928970023113	38.0	35.0	40.0	31.0	41.0
20	36.38165673039256	38.0	35.0	40.0	30.0	41.0
21	36.2245026748233	38.0	34.0	40.0	30.0	41.0
22	36.148838973866354	38.0	34.0	40.0	30.0	41.0
23	36.10393769743994	38.0	34.0	40.0	30.0	41.0
24	35.92127529774839	38.0	34.0	40.0	30.0	41.0
25	35.78963041014781	37.0	34.0	40.0	29.0	41.0
26	35.71093660213615	37.0	34.0	40.0	29.0	41.0
27	35.70045892363644	37.0	34.0	40.0	29.0	41.0
28	35.72378448672516	37.0	34.0	40.0	29.0	41.0
29	35.76798055063422	37.0	34.0	40.0	29.0	41.0
30	35.69669147405481	37.0	34.0	40.0	29.0	41.0
31	35.572563360996725	37.0	34.0	40.0	29.0	41.0
32	35.43016274673685	37.0	34.0	40.0	29.0	41.0
33	35.31694895076982	37.0	34.0	40.0	27.0	41.0
34	35.17278118598456	37.0	34.0	40.0	27.0	41.0
35	35.02981252963273	37.0	34.0	40.0	26.0	41.0
36	34.83730804893813	37.0	33.0	40.0	25.0	41.0
37	34.76519306634394	37.0	33.0	40.0	25.0	41.0
38	34.683544121299846	37.0	33.0	40.0	25.0	41.0
39	34.64153619342595	37.0	33.0	40.0	24.0	41.0
40	34.50414868450267	37.0	33.0	40.0	24.0	41.0
41	34.44028781897788	36.0	33.0	40.0	24.0	41.0
42	34.39004348673216	36.0	33.0	40.0	24.0	41.0
43	34.28507927667934	36.0	33.0	40.0	24.0	41.0
44	34.148548156087585	36.0	33.0	40.0	23.0	41.0
45	34.06426537410659	36.0	33.0	40.0	23.0	41.0
46	33.95290770407602	36.0	33.0	40.0	23.0	41.0
47	33.9046270097218	35.0	33.0	40.0	23.0	41.0
48	33.8011329535676	35.0	33.0	40.0	23.0	41.0
49	33.69439129490941	36.0	32.0	40.0	23.0	41.0
50	33.538837972892985	35.0	32.0	39.0	23.0	41.0
51	33.17158125370474	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	5.0
12	11.0
13	9.0
14	37.0
15	86.0
16	289.0
17	663.0
18	1467.0
19	2776.0
20	4779.0
21	7721.0
22	11633.0
23	16458.0
24	22432.0
25	30868.0
26	40919.0
27	48893.0
28	55617.0
29	64248.0
30	75866.0
31	92453.0
32	113119.0
33	142344.0
34	219400.0
35	282656.0
36	192601.0
37	233399.0
38	323312.0
39	443510.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.19132967572994	24.222773009309055	29.039844095307494	13.546053219653514
2	30.228324910190445	25.920390898639294	29.876171767031067	13.975112424139194
3	29.242592694047755	25.186796872843843	31.55957830598232	14.011032127126091
4	25.794301207305704	28.830793071616554	30.407017194086265	14.967888526991475
5	23.305337659625387	32.39005666827454	29.86286664769074	14.441739024409333
6	22.541467278674695	40.656819779892956	26.57003497639886	10.231677965033487
7	88.36926608055488	3.884724116496824	6.122867628067953	1.6231421748803467
8	89.07942167630497	2.9850426567069133	6.002009361366629	1.9335263056214747
9	83.71684069735302	5.661513644750018	7.805944628459692	2.8157010294372675
10	43.57558399381786	31.374130481616486	13.716754193481151	11.333531331084508
11	37.9654371720319	22.682592166786055	24.17012922442688	15.181841436755168
12	34.32016401134107	22.16987136050406	26.82868155329648	16.681283074858392
13	24.17704953417665	31.30657507691636	27.625382213238638	16.890993175668356
14	18.552608977371822	34.92997511571952	28.913342480774514	17.604073426134136
15	17.28170233029073	25.534089322250402	40.6968999071937	16.487308440265164
16	20.791164412142344	23.16413038687415	38.7315319382593	17.313173262724206
17	21.5402467502349	22.578169635740434	28.577954611830354	27.303629002194313
18	22.92710977794456	24.651502675235218	32.88794823937845	19.533439307441764
19	27.0283407280413	26.251165227750278	27.160238536486304	19.56025550772212
20	29.126759890379	25.443754564623955	27.089140592271416	18.340344952725633
21	24.65277963715333	27.039091923545406	29.612746881020513	18.69538155828075
22	24.366287051976883	23.32276201095963	28.891386974246974	23.419563962816518
23	21.4183586755351	27.924108917436996	28.046697261575765	22.61083514545214
24	20.9058438308528	25.2272065387041	34.83061924002641	19.03633039041669
25	20.27481868170571	26.469690485027208	32.477260809585616	20.778230023681466
26	19.71740420828979	31.883844248542925	27.682763114913804	20.715988428253482
27	19.198710515616625	31.592285008013967	29.797823974506898	19.41118050186251
28	17.9696964579136	28.00608163411581	34.67969057976955	19.344531328201047
29	19.075586671318653	25.414425632827314	33.94457243813635	21.56541525771769
30	19.945032968273264	28.529141712702515	31.400493566377506	20.125331752646712
31	25.62681323443332	26.297506587681767	28.081175233364792	19.994504944520124
32	26.738346795670026	26.39546192449695	28.117630436510893	18.748560843322128
33	25.500970697019365	27.05490977440202	27.22009097735782	20.224028551220798
34	20.079484700554488	27.194181008116125	29.450037217261066	23.27629707406832
35	20.214307163715166	27.042387309140537	30.921303308525943	21.82200221861835
36	26.24778745751527	26.070660481777136	28.367049933742155	19.31450212696544
37	21.188340760995157	29.933593861026175	29.594951798806825	19.283113579171847
38	20.869429820026635	30.709492399399085	26.360736798788288	22.06034098178599
39	21.024807250836925	29.394468777663217	28.517690247759447	21.06303372374041
40	23.293721425402563	26.521551615830536	27.59535301200303	22.589373946763867
41	19.14219465266018	25.87936334797995	29.42775217217401	25.550689827185863
42	21.887374430361707	26.39410257793896	27.209916474332857	24.508606517366477
43	21.98162245838237	26.143735657349097	28.260938517579028	23.6137033666895
44	19.92946227133628	28.415368525030722	29.295936748368888	22.35923245526411
45	19.381604416146235	32.25016754976136	26.05035266804716	22.317875366045254
46	22.129544079283683	29.980141182557357	27.363934558585157	20.5263801795738
47	20.950125574787336	27.164934460959362	28.9249175226774	22.9600224415759
48	21.758195315032683	26.160377354604496	30.860297482696136	21.22112984766668
49	21.6064016160571	25.181071140372303	30.955698895675095	22.256828347895503
50	20.295044110795807	29.768577427350134	28.531036559419714	21.405341902434344
51	19.35528252370515	30.530264615344056	27.51803502747734	22.596417833473456
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1475.0
1	2316.0
2	3157.0
3	11620.5
4	20084.0
5	14491.0
6	8898.0
7	9078.5
8	9259.0
9	10178.5
10	11098.0
11	11396.0
12	11694.0
13	11831.5
14	11969.0
15	11020.5
16	10072.0
17	9637.5
18	9203.0
19	9566.5
20	9930.0
21	10511.5
22	11093.0
23	12026.0
24	12959.0
25	15973.0
26	20575.5
27	22164.0
28	26634.5
29	31105.0
30	37537.0
31	43969.0
32	52061.5
33	60154.0
34	64896.0
35	69638.0
36	79552.5
37	89467.0
38	98546.0
39	107625.0
40	129806.5
41	151988.0
42	179265.5
43	206543.0
44	212557.0
45	218571.0
46	212043.5
47	205516.0
48	203744.0
49	201972.0
50	190449.0
51	178926.0
52	163128.5
53	147331.0
54	134079.5
55	120828.0
56	110590.5
57	100353.0
58	92729.0
59	85105.0
60	78522.0
61	71939.0
62	61574.0
63	51209.0
64	44727.0
65	38245.0
66	33801.5
67	29358.0
68	23641.0
69	17924.0
70	14964.0
71	12004.0
72	9520.0
73	7036.0
74	5722.0
75	3140.0
76	1872.0
77	1564.5
78	1257.0
79	992.5
80	728.0
81	526.0
82	324.0
83	215.0
84	106.0
85	80.5
86	55.0
87	32.5
88	10.0
89	12.0
90	14.0
91	12.0
92	10.0
93	7.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2427637.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.99422234839405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.71434944669045	17.155292213381898
2	7.122299970257699	2.99054298415101
3	2.1750446089098463	1.3699011041138727
4	1.066225541043129	0.8953830432878479
5	0.6200392700785735	0.6508621150382762
6	0.43375215414434753	0.5463773500920794
7	0.32166659500779227	0.4727198012351087
8	0.25228224570904734	0.42371756487743373
9	0.20908321920713463	0.39505856340173384
>10	3.2628047204346236	17.907728548260057
>50	1.8057681738236953	27.163917027011227
>100	1.0139148770276276	29.07709207181883
>500	0.0023735808565862526	0.3308395292831334
>1k	1.9779840471552106E-4	0.13060001501135368
>5k	0.0	0.0
>10k+	1.9779840471552106E-4	0.48996806903623585
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11799	0.48602818296145595	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3145	0.1295498462084735	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.235769598173038E-4	0.0	0.0	0.037649780424338565	0.0
2	1.235769598173038E-4	0.0	0.0	0.1262956529332845	0.0
3	1.235769598173038E-4	0.0	0.0	0.18812532516187552	0.0
4	1.235769598173038E-4	0.0	0.0	0.31059009234082363	0.0
5	1.235769598173038E-4	0.0	0.0	0.5432855076768067	0.0
6	1.235769598173038E-4	0.0	0.0	0.8779319148620655	0.0
7	1.235769598173038E-4	0.0	0.0	1.0506925046866562	0.0
8	1.235769598173038E-4	0.0	0.0	1.4933451747522386	0.0
9	1.235769598173038E-4	0.0	0.0	1.6472396820447208	0.0
10	1.235769598173038E-4	0.0	0.0	1.912435837812655	0.0
11	1.235769598173038E-4	0.0	0.0	2.2273923160670233	0.0
12	1.235769598173038E-4	0.0	0.0	2.4841440462474416	0.0
13	1.235769598173038E-4	0.0	0.0	2.600306388475707	0.0
14	1.235769598173038E-4	0.0	0.0	2.6489957106437245	0.0
15	1.235769598173038E-4	0.0	0.0	2.7146974609465913	0.0
16	1.235769598173038E-4	0.0	0.0	2.8636900821663205	0.0
17	1.235769598173038E-4	0.0	0.0	3.028088631043274	0.0
18	1.235769598173038E-4	0.0	0.0	3.2289011907463925	0.0
19	1.235769598173038E-4	0.0	0.0	3.345722610093684	0.0
20	1.235769598173038E-4	0.0	0.0	3.46917599295117	0.0
21	1.235769598173038E-4	0.0	0.0	3.635798927104835	0.0
22	1.235769598173038E-4	0.0	0.0	3.8079828244502782	0.0
23	1.235769598173038E-4	0.0	0.0	3.9965200728115446	0.0
24	1.235769598173038E-4	0.0	0.0	4.141723000596877	0.0
25	1.235769598173038E-4	0.0	0.0	4.26624738377278	0.0
26	1.235769598173038E-4	0.0	0.0	4.386982073514286	0.0
27	1.235769598173038E-4	0.0	0.0	4.509529225333112	0.0
28	1.235769598173038E-4	0.0	0.0	4.643074726575678	0.0
29	1.235769598173038E-4	0.0	0.0	4.785888499804543	0.0
30	1.235769598173038E-4	0.0	0.0	4.964869129939937	0.0
31	1.235769598173038E-4	0.0	0.0	5.1261782548214585	0.0
32	1.235769598173038E-4	0.0	0.0	5.270227797648495	0.0
33	1.235769598173038E-4	0.0	0.0	5.423216073902317	0.0
34	1.235769598173038E-4	0.0	0.0	5.572661810641377	0.0
35	1.235769598173038E-4	0.0	0.0	5.760539981883618	0.0
36	1.235769598173038E-4	0.0	0.0	5.927080531397404	0.0
37	1.235769598173038E-4	0.0	0.0	6.104578238014992	0.0
38	1.235769598173038E-4	0.0	0.0	6.2882547926234444	0.0
39	1.235769598173038E-4	0.0	0.0	6.518313899483324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7685	0.0	41.310997	1
TCGTAAT	50	1.0822987E-9	40.5	28
TCACGAC	235	0.0	40.212765	25
GGGCGAT	3810	0.0	39.980316	7
CGACCCG	210	0.0	39.642853	32
GCGATAA	205	0.0	39.51219	9
ACGGGAT	825	0.0	39.272728	5
GCGATAT	275	0.0	39.272728	9
CGAAAGG	475	0.0	38.842106	2
TACGGGA	995	0.0	38.668343	4
GGCGATA	890	0.0	38.174156	8
CTTAACG	65	9.094947E-12	38.07692	1
TACGCAA	30	1.1402959E-4	37.499996	28
CGCTTAA	30	1.1402959E-4	37.499996	40
AGGGCGA	1985	0.0	37.292194	6
ACCGTTC	85	0.0	37.058826	43
TAAGGGA	3235	0.0	36.93199	4
CGAACGG	195	0.0	36.923077	2
CACGACG	250	0.0	36.899998	26
TATCGGA	55	2.752131E-9	36.818184	37
>>END_MODULE