FastQCFastQC Report
Thu 26 May 2016
SRR1548178_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548178_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1863394
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227331.219978168868205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG750.045.01
CGTTTTT126750.043.1893461
ACGGGAC5650.039.823015
GGGCGAT27000.038.757
TACGGGT1750.038.571434
ATAGGGA24300.038.4259264
CGGTCTA1650.038.18181631
AGGGCGA15000.037.656
TATCGCG301.1401941E-437.5000041
GCGTAAG2050.037.3170741
CGTTAGG2900.037.241382
CGAGGGA12100.037.0041354
TAGGGAG24400.036.8852465
TAGTACG1100.036.818181
TAGGGAC16950.036.6371655
AAGGGAT25450.036.601185
GTTTTTT155950.036.3578072
TCGTTAG1300.036.3461531
CGAGGGT4150.036.32534
AGGGATT27100.036.2822886
Produced by FastQC (version 0.11.5)