##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548178_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1863394
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.146717226737877	31.0	31.0	33.0	30.0	34.0
2	31.538663320800648	31.0	31.0	34.0	30.0	34.0
3	31.56256808812307	31.0	31.0	34.0	30.0	34.0
4	35.42768088767056	37.0	35.0	37.0	33.0	37.0
5	35.31900231513035	37.0	35.0	37.0	33.0	37.0
6	35.37108255151621	37.0	35.0	37.0	33.0	37.0
7	35.76990802803916	37.0	35.0	37.0	35.0	37.0
8	35.76295136723635	37.0	35.0	37.0	35.0	37.0
9	37.51773484298007	39.0	37.0	39.0	35.0	39.0
10	36.79170535055925	39.0	37.0	39.0	32.0	39.0
11	36.53333326177931	39.0	35.0	39.0	32.0	39.0
12	36.06838328340652	38.0	35.0	39.0	31.0	39.0
13	35.89769742738251	38.0	35.0	39.0	31.0	39.0
14	36.846462959524395	39.0	35.0	41.0	31.0	41.0
15	37.02095155399234	39.0	35.0	41.0	31.0	41.0
16	37.081889820402985	39.0	35.0	41.0	32.0	41.0
17	36.910586810948196	39.0	35.0	41.0	31.0	41.0
18	36.794740135473226	38.0	35.0	40.0	31.0	41.0
19	36.69338422255304	38.0	35.0	40.0	31.0	41.0
20	36.533448105982956	38.0	35.0	40.0	31.0	41.0
21	36.40989130586446	38.0	35.0	40.0	30.0	41.0
22	36.35674312571577	38.0	35.0	40.0	30.0	41.0
23	36.27436977901614	38.0	35.0	40.0	30.0	41.0
24	36.10672353780252	38.0	35.0	40.0	30.0	41.0
25	35.98794350523829	38.0	34.0	40.0	30.0	41.0
26	35.937108845472295	38.0	34.0	40.0	30.0	41.0
27	35.927408266850705	38.0	34.0	40.0	30.0	41.0
28	35.927833297735205	38.0	34.0	40.0	30.0	41.0
29	35.89817880705852	38.0	34.0	40.0	30.0	41.0
30	35.78570822917751	38.0	34.0	40.0	29.0	41.0
31	35.64694852511063	38.0	34.0	40.0	29.0	41.0
32	35.446236276385996	38.0	34.0	40.0	28.0	41.0
33	35.22789114916115	38.0	34.0	40.0	27.0	41.0
34	34.90885287813527	38.0	34.0	40.0	24.0	41.0
35	34.676021818252075	38.0	33.0	40.0	24.0	41.0
36	34.52551741606982	37.0	33.0	40.0	23.0	41.0
37	34.45207669446182	37.0	33.0	40.0	23.0	41.0
38	34.35200231405704	37.0	33.0	40.0	23.0	41.0
39	34.28057136601277	37.0	33.0	40.0	23.0	41.0
40	34.159665642370854	37.0	33.0	40.0	23.0	41.0
41	34.07940188709419	37.0	33.0	40.0	22.0	41.0
42	34.02364126964024	37.0	33.0	40.0	22.0	41.0
43	33.92308658286975	37.0	33.0	40.0	22.0	41.0
44	33.7716843566095	36.0	33.0	40.0	21.0	41.0
45	33.68585709731812	36.0	33.0	40.0	20.0	41.0
46	33.57458594371346	36.0	32.0	40.0	20.0	41.0
47	33.5509725801414	36.0	32.0	40.0	20.0	41.0
48	33.44090085081309	36.0	32.0	40.0	20.0	41.0
49	33.335172271672015	36.0	32.0	40.0	20.0	41.0
50	33.225097859067915	35.0	32.0	40.0	20.0	41.0
51	32.88701369651292	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	3.0
13	7.0
14	31.0
15	89.0
16	228.0
17	520.0
18	1201.0
19	2222.0
20	3863.0
21	6159.0
22	9173.0
23	13296.0
24	19047.0
25	27515.0
26	38037.0
27	44866.0
28	47126.0
29	50849.0
30	56898.0
31	66294.0
32	80669.0
33	101531.0
34	152907.0
35	185583.0
36	149602.0
37	184537.0
38	258981.0
39	362105.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.069056785628806	24.629520112225325	28.365927978731282	16.935495123414587
2	32.99892561637528	25.37616843244102	29.141072687794424	12.483833263389279
3	27.275820357906056	24.71302365468602	34.90378309686518	13.10737289054274
4	24.16923098389283	27.24034745201498	34.68262750658207	13.907794057510115
5	22.197291608752632	31.27535024798835	33.16942095981848	13.357937183440537
6	20.963467736828605	39.27306839025992	29.83775841287457	9.9257054600369
7	85.94467943977494	3.1568202967273695	9.584124452477576	1.3143758110201065
8	87.06655704590656	2.380816939412706	9.291271733192229	1.2613542814885097
9	82.93023375625337	4.435240212214915	10.504380716048244	2.1301453154834675
10	39.16525436917796	30.597554784441723	18.015728289347287	12.221462557033027
11	33.04175069792003	22.964547487004896	28.08128608335113	15.91241573172394
12	31.45743734282712	20.98734889132411	29.985982567293874	17.569231198554895
13	24.391781877584666	25.291537914150204	31.22426067702268	19.092419531242452
14	19.15510085360369	28.251835092310053	32.80411979431081	19.78894425977544
15	19.581527041516715	24.882553018846256	38.38254282239827	17.153377117238758
16	24.058787352540577	23.291692470835475	35.88081747606786	16.768702700556084
17	22.885820175443303	23.441472925210665	31.635445858471158	22.037261040874874
18	24.017464905435997	24.455160851650266	33.60239434064937	17.92497990226436
19	25.72145236058504	26.278071089635365	29.527249738917266	18.473226810862332
20	27.386049327195426	25.847995646653366	29.762573025350513	17.00338200080069
21	24.920977528101947	25.556967554902503	31.980944448678056	17.541110468317488
22	24.01649892615303	22.601768600736076	32.98126966170332	20.40046281140757
23	22.627957372407554	24.935628213893573	32.06959988064789	20.36681453305098
24	22.396766330684763	23.977269434161535	35.08951944677293	18.53644478838077
25	21.465508636391444	25.317082699633037	32.74524872356571	20.472159940409814
26	19.83316464472892	28.330723400418805	31.222114056393867	20.613997898458404
27	19.29146492904882	27.94223873211999	32.79451366699689	19.971782671834298
28	18.436143939499644	27.577635218316683	34.37791470832256	19.608306133861117
29	20.484020019383983	24.906917162983245	34.488412005190526	20.120650812442243
30	21.33247182292097	26.038132568850177	34.0245809528205	18.604814655408358
31	25.050633414082046	24.9065415043732	31.2993387335153	18.743486348029457
32	25.69247298209611	25.19445699621229	30.56959505075148	18.543474970940125
33	24.923124148730757	26.27302653115766	29.408487952628377	19.395361367483204
34	21.092855295230102	26.374508021384635	32.27739275751666	20.255243925868605
35	20.519868583885103	27.98034124828136	31.83583289417053	19.663957273663005
36	23.64250394709868	28.602592903057538	28.83657455159778	18.918328598245996
37	22.272047672150926	29.28634523884911	29.616119832949984	18.825487256049982
38	21.86504840092863	29.857829315753943	28.025366615970643	20.25175566734679
39	21.8366056775969	28.518391708892484	29.074473782785603	20.57052883072501
40	23.12758332376298	26.370858766315656	28.73648836477954	21.76506954514182
41	19.881731936455736	26.459138539675454	29.714971712906664	23.944157810962146
42	21.17276324813754	26.625233310829593	28.5086245850314	23.693378856001466
43	21.223906484618926	26.428173537104875	28.899094877411862	23.448825100864337
44	20.760290094311777	27.582196787152906	29.022793891147014	22.634719227388302
45	20.713171771509405	29.56256164826118	27.954420804188484	21.76984577604092
46	21.376370214780128	29.122075095229455	28.79267615973863	20.708878530251788
47	20.72691014353379	27.66811527782101	29.47664315759308	22.12833142105212
48	21.077936281859873	25.994985494211097	31.747016465653534	21.18006175827549
49	21.77832492752472	25.322663913267938	31.55795285377113	21.34105830543621
50	20.383504508439977	27.585846042221878	30.61215180471763	21.418497644620516
51	19.451388165895136	28.235306113468216	30.30303843416905	22.010267286467595
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1031.0
1	1786.0
2	2541.0
3	20021.5
4	37502.0
5	26244.5
6	14987.0
7	14505.5
8	14024.0
9	14363.0
10	14702.0
11	14845.0
12	14988.0
13	14525.0
14	14062.0
15	13034.5
16	12007.0
17	11255.0
18	10503.0
19	9973.5
20	9444.0
21	9479.0
22	9514.0
23	10773.5
24	12033.0
25	12678.5
26	16832.5
27	20341.0
28	23200.5
29	26060.0
30	31116.5
31	36173.0
32	41303.5
33	46434.0
34	51040.0
35	55646.0
36	60818.5
37	65991.0
38	71870.0
39	77749.0
40	90772.5
41	103796.0
42	115017.0
43	126238.0
44	130587.5
45	134937.0
46	134983.0
47	135029.0
48	133315.5
49	131602.0
50	128951.5
51	126301.0
52	118612.5
53	110924.0
54	105996.5
55	101069.0
56	94994.0
57	88919.0
58	84776.5
59	80634.0
60	73978.5
61	67323.0
62	59900.5
63	52478.0
64	45167.0
65	37856.0
66	30416.0
67	22976.0
68	18567.0
69	14158.0
70	11235.0
71	8312.0
72	6845.5
73	5379.0
74	4222.5
75	2359.5
76	1653.0
77	1313.5
78	974.0
79	666.5
80	359.0
81	267.0
82	175.0
83	140.5
84	106.0
85	69.5
86	33.0
87	25.5
88	18.0
89	12.5
90	7.0
91	7.5
92	8.0
93	5.5
94	3.0
95	4.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1863394.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.539558600641733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.47126701734986	19.25650255438862
2	5.375714324487894	2.6383531337215973
3	2.171124410091538	1.59835304072175
4	1.2839433511481466	1.2602961242161708
5	0.8789818859963062	1.0784913750154472
6	0.7210408119599838	1.0616413955127793
7	0.6084763377929127	1.0452218523861139
8	0.546633523625643	1.0731316308869394
9	0.5070439404751237	1.1198371041350634
>10	8.2466716242801	48.48456572656124
>50	1.1295720756648435	17.890752609272376
>100	0.05842827771701003	2.102335126629815
>500	6.614516464011702E-4	0.09181749416961381
>1k	2.204838821337234E-4	0.06871432975569212
>5k	0.0	0.0
>10k+	2.204838821337234E-4	1.2299865026268892
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22733	1.219978168868205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0733103144047904E-4	0.0	0.0	0.017977947766280238	0.0
2	1.0733103144047904E-4	0.0	0.0	0.05602679841193006	0.0
3	1.0733103144047904E-4	0.0	0.0	0.09284134219601436	0.0
4	1.0733103144047904E-4	0.0	0.0	0.13486144100496192	0.0
5	1.0733103144047904E-4	0.0	0.0	0.23607460365333366	0.0
6	1.0733103144047904E-4	0.0	0.0	0.43039743607632097	0.0
7	1.0733103144047904E-4	0.0	0.0	0.5591410082891756	0.0
8	1.0733103144047904E-4	0.0	0.0	0.7996161842315689	0.0
9	1.0733103144047904E-4	0.0	0.0	0.9373755630854237	0.0
10	1.0733103144047904E-4	0.0	0.0	1.1223605957730893	0.0
11	1.0733103144047904E-4	0.0	0.0	1.3003154459014035	0.0
12	1.0733103144047904E-4	0.0	0.0	1.4260000837182045	0.0
13	1.0733103144047904E-4	0.0	0.0	1.4878227578279204	0.0
14	1.0733103144047904E-4	0.0	0.0	1.5260862705364513	0.0
15	1.0733103144047904E-4	0.0	0.0	1.562095831584732	0.0
16	1.0733103144047904E-4	0.0	0.0	1.624830819461692	0.0
17	1.0733103144047904E-4	0.0	0.0	1.6978159208412178	0.0
18	1.0733103144047904E-4	0.0	0.0	1.7939308594961667	0.0
19	1.0733103144047904E-4	0.0	0.0	1.8536605784927933	0.0
20	1.0733103144047904E-4	0.0	0.0	1.9189178456086045	0.0
21	1.0733103144047904E-4	0.0	0.0	2.00306537425794	0.0
22	1.0733103144047904E-4	0.0	0.0	2.099126647397169	0.0
23	1.0733103144047904E-4	0.0	0.0	2.1951879205363976	0.0
24	1.0733103144047904E-4	0.0	0.0	2.279228118154293	0.0
25	1.0733103144047904E-4	0.0	0.0	2.354359840162628	0.0
26	1.0733103144047904E-4	0.0	0.0	2.4256813105548263	0.0
27	1.0733103144047904E-4	0.0	0.0	2.502530329066209	0.0
28	1.0733103144047904E-4	0.0	0.0	2.5778767131374254	0.0
29	1.0733103144047904E-4	0.0	0.0	2.6710937139434816	0.0
30	1.0733103144047904E-4	0.0	0.0	2.7804103694656095	0.0
31	1.0733103144047904E-4	0.0	0.0	2.8778132804978442	0.0
32	1.0733103144047904E-4	0.0	0.0	2.9782214604104125	0.0
33	1.0733103144047904E-4	0.0	0.0	3.0723507749837125	0.0
34	1.0733103144047904E-4	0.0	0.0	3.1805404546757154	0.0
35	1.0733103144047904E-4	0.0	0.0	3.298765585807403	0.0
36	1.0733103144047904E-4	0.0	0.0	3.4086725620024536	0.0
37	1.0733103144047904E-4	0.0	0.0	3.5259317138511768	0.0
38	1.0733103144047904E-4	0.0	0.0	3.6804347336097467	0.0
39	1.0733103144047904E-4	0.0	0.0	3.9060982272133535	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	75	0.0	45.0	1
CGTTTTT	12675	0.0	43.189346	1
ACGGGAC	565	0.0	39.82301	5
GGGCGAT	2700	0.0	38.75	7
TACGGGT	175	0.0	38.57143	4
ATAGGGA	2430	0.0	38.425926	4
CGGTCTA	165	0.0	38.181816	31
AGGGCGA	1500	0.0	37.65	6
TATCGCG	30	1.1401941E-4	37.500004	1
GCGTAAG	205	0.0	37.317074	1
CGTTAGG	290	0.0	37.24138	2
CGAGGGA	1210	0.0	37.004135	4
TAGGGAG	2440	0.0	36.885246	5
TAGTACG	110	0.0	36.81818	1
TAGGGAC	1695	0.0	36.637165	5
AAGGGAT	2545	0.0	36.60118	5
GTTTTTT	15595	0.0	36.357807	2
TCGTTAG	130	0.0	36.346153	1
CGAGGGT	415	0.0	36.3253	4
AGGGATT	2710	0.0	36.282288	6
>>END_MODULE