FastQCFastQC Report
Thu 26 May 2016
SRR1548177_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548177_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences926780
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80650.8702173115518247No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48020.5181380694447442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGG453.8380676E-1045.0000042
TATCGTG453.8380676E-1045.0000041
CGGGTAG207.032579E-445.06
CGTGGCT207.032579E-445.022
CTGTCGT406.8121153E-945.037
ACACGAA207.032579E-445.036
GTCGACC253.8902188E-545.015
CGAGTCA207.032579E-445.014
GGTCGAC253.8902188E-545.014
CCCTAGT253.8902188E-545.035
AACCCGT253.8902188E-545.037
CGGTTGC207.032579E-445.031
CGTGACG253.8902188E-545.019
GGCGTGT253.8902188E-545.08
GACCGTA253.8902188E-545.026
GAGTACG551.8189894E-1245.01
ATTACGC253.8902188E-545.028
TTGTCGA406.8121153E-945.01
CAACCCG253.8902188E-545.01
ACTTCGT253.8902188E-545.037