##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548177_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	926780
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13713934267032	31.0	31.0	33.0	30.0	34.0
2	31.503839098815252	31.0	31.0	34.0	30.0	34.0
3	31.55893308012689	31.0	31.0	34.0	30.0	34.0
4	35.38211981268478	37.0	35.0	37.0	33.0	37.0
5	35.23455620535618	37.0	35.0	37.0	33.0	37.0
6	35.290274930404195	37.0	35.0	37.0	32.0	37.0
7	35.760524612097804	37.0	35.0	37.0	35.0	37.0
8	35.77858067718336	37.0	35.0	37.0	35.0	37.0
9	37.568781156261466	39.0	37.0	39.0	35.0	39.0
10	36.81459569692915	39.0	37.0	39.0	32.0	39.0
11	36.40630462461426	38.0	35.0	39.0	32.0	39.0
12	35.08700554608429	35.0	35.0	39.0	30.0	39.0
13	34.3904907313494	35.0	33.0	39.0	25.0	39.0
14	35.17326981592179	36.0	33.0	40.0	25.0	41.0
15	35.73444398886467	36.0	33.0	40.0	30.0	41.0
16	36.04902889574657	36.0	34.0	40.0	31.0	41.0
17	35.97875115993008	36.0	34.0	40.0	31.0	41.0
18	35.925436457411685	36.0	35.0	40.0	31.0	41.0
19	35.78504283648763	36.0	34.0	40.0	30.0	41.0
20	35.56474136256717	35.0	34.0	40.0	30.0	41.0
21	35.35016400871836	35.0	34.0	40.0	30.0	41.0
22	35.2309480135523	35.0	34.0	40.0	29.0	41.0
23	35.298117136753056	35.0	34.0	40.0	30.0	41.0
24	35.1094100002158	35.0	34.0	39.0	29.0	41.0
25	34.97391506074796	35.0	34.0	39.0	29.0	41.0
26	34.86689937201925	35.0	34.0	39.0	29.0	41.0
27	34.805651826755	35.0	34.0	39.0	29.0	41.0
28	34.915031614838476	35.0	34.0	39.0	29.0	41.0
29	35.04437514836315	35.0	34.0	39.0	29.0	41.0
30	35.028458749649324	36.0	34.0	39.0	29.0	41.0
31	34.771008222016015	35.0	34.0	39.0	29.0	41.0
32	34.51137270981247	35.0	33.0	39.0	27.0	41.0
33	34.35715056431947	35.0	33.0	39.0	27.0	41.0
34	34.24071300632297	35.0	34.0	39.0	26.0	41.0
35	34.06761367314789	35.0	33.0	39.0	25.0	41.0
36	33.74814303286649	35.0	33.0	39.0	23.0	41.0
37	33.63443859384104	35.0	33.0	39.0	23.0	41.0
38	33.605349705431706	35.0	33.0	39.0	23.0	41.0
39	33.65597013314918	35.0	33.0	39.0	23.0	41.0
40	33.46621528302294	35.0	33.0	39.0	23.0	41.0
41	33.49513584669501	35.0	33.0	39.0	23.0	41.0
42	33.45024601307754	35.0	33.0	39.0	23.0	41.0
43	33.30462461425581	35.0	33.0	39.0	23.0	41.0
44	33.155045426098965	35.0	32.0	38.0	23.0	41.0
45	33.02744448520684	35.0	32.0	38.0	23.0	40.0
46	32.94926627678629	35.0	32.0	38.0	23.0	40.0
47	32.934153736593366	35.0	32.0	38.0	23.0	40.0
48	32.88415265758864	35.0	32.0	38.0	23.0	40.0
49	32.86315090960098	35.0	32.0	38.0	22.0	40.0
50	32.663246941021605	35.0	32.0	38.0	21.0	40.0
51	32.28752238934806	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	11.0
15	25.0
16	90.0
17	249.0
18	618.0
19	1152.0
20	2014.0
21	3305.0
22	5043.0
23	7130.0
24	10260.0
25	14242.0
26	18700.0
27	22102.0
28	24129.0
29	27346.0
30	32084.0
31	38930.0
32	48881.0
33	63651.0
34	112784.0
35	163129.0
36	60160.0
37	65580.0
38	86227.0
39	118903.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.693778458749648	24.93979153628693	30.9623643151557	14.404065689807721
2	29.32529834480675	26.565420056539846	31.40270614385291	12.706575454800491
3	27.48796909730465	25.39189451649798	33.965666069617384	13.154470316579985
4	23.648654481106625	28.553594164742442	33.53589848723537	14.261852866915556
5	22.570620859319362	32.070717969744706	32.3887006625089	12.969960508427029
6	20.07132221239129	41.8744470100779	27.958846759748806	10.095384017782
7	87.0361898185114	4.227432616154859	7.246056237726322	1.490321327607415
8	89.07669565592697	2.6567254364574118	6.430436565312156	1.836142342303459
9	86.51416733205292	3.654589007099851	8.001467446427416	1.829776214419819
10	57.219189020047914	19.676514383132996	13.112604933209607	9.991691663609487
11	51.615485875828135	19.79801031528518	18.427458512268284	10.1590452966184
12	47.273031355877336	20.886726083860246	21.19219232180237	10.648050238460044
13	19.79747081292216	47.41880489436544	21.92947625110598	10.854248041606422
14	12.001122164915081	49.65374738341354	26.353719329290666	11.991411122380716
15	10.474114676622284	24.586633289453808	53.52564794233798	11.413604091585922
16	11.94749563003086	18.058115194544552	53.80262845551263	16.191760719911954
17	12.878892509549193	18.497270118043115	28.60916290813354	40.01467446427415
18	20.11577720710417	24.819158807915578	36.38889488335959	18.676169101620665
19	28.779645654847968	26.56790176740974	25.589783983253845	19.062668594488443
20	31.215606724357453	23.60107037268823	25.167677334426724	20.01564556852759
21	19.13096959364682	30.102397548501266	30.036254558794965	20.73037829905695
22	21.16780681499385	24.8038369408058	27.288029521569307	26.740326722631046
23	16.787155527741213	33.43371673967932	24.484775243315564	25.294352489263904
24	18.673903191695977	23.718789788299272	41.91221217548933	15.69509484451542
25	14.42122186495177	25.49439996547185	39.214592459914975	20.86978570966141
26	13.318047433047756	39.1277325794687	27.709380867088196	19.844839120395346
27	14.344180927512461	39.30156024083386	30.236625736420724	16.117633095232957
28	11.26588834459095	31.53704223224498	43.05779149312674	14.139277930037334
29	11.332894538078078	26.012322233971386	42.48354517792788	20.17123805002266
30	15.654740067761496	34.3133213923477	33.62362157146248	16.40831696842832
31	29.559010768467164	29.227756317572673	24.63702281015991	16.576210103800253
32	29.42521418243812	29.595804829625155	25.70728759791968	15.271693390017049
33	26.24398454865232	30.43192559183409	24.15718940848961	19.166900451023974
34	16.41468309631196	31.605774833293772	27.753943762273682	24.22559830812059
35	16.621636202766567	28.894775459116513	31.755648589740826	22.727939748376098
36	30.92503075163469	25.454476790608343	27.215196702561556	16.405295755195407
37	17.18681887826669	34.78894667558644	31.144176611493556	16.880057834653318
38	17.387190055892447	35.12926476617967	24.008934159131616	23.474611018796264
39	17.478365955242882	33.64617277023673	29.08392498759145	19.791536286928938
40	22.60180409590194	27.693951099505814	25.871512117223073	23.83273268736917
41	14.644899544660007	24.6182481279268	28.90103368652755	31.83581864088565
42	21.38360776020199	25.613090485336325	25.30438723321608	27.698914521245605
43	22.10470661861499	26.91242797643454	26.023435982649605	24.959429422300868
44	17.44750642007812	33.77435853169037	28.219210600142432	20.558924448089083
45	14.718919268866399	43.14033535466885	20.807203435551045	21.3335419409137
46	22.229115863527483	36.28013120697469	24.46664796391808	17.02410496557975
47	20.65376896350806	30.783033729687737	25.5487818036643	23.014415503139904
48	21.97608925527094	25.989771035197133	31.200069056302464	20.83407065322946
49	20.27072228576361	24.344504628930274	33.09231964436004	22.292453440946073
50	17.706143852910074	35.80655603271543	27.66276786292324	18.824532251451263
51	16.61505427393772	36.874231209132695	23.579490278167416	22.931224238762166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	892.0
1	981.0
2	1070.0
3	5717.5
4	10365.0
5	7066.0
6	3767.0
7	4308.0
8	4849.0
9	5695.0
10	6541.0
11	7338.5
12	8136.0
13	8065.5
14	7995.0
15	7753.5
16	7512.0
17	7107.0
18	6702.0
19	6061.5
20	5421.0
21	5658.5
22	5896.0
23	5484.5
24	5073.0
25	5114.0
26	6109.5
27	7064.0
28	7852.0
29	8640.0
30	11091.5
31	13543.0
32	15364.0
33	17185.0
34	18891.0
35	20597.0
36	22395.0
37	24193.0
38	31176.0
39	38159.0
40	58815.0
41	79471.0
42	95386.0
43	111301.0
44	113280.5
45	115260.0
46	107953.5
47	100647.0
48	92547.0
49	84447.0
50	78227.0
51	72007.0
52	60744.5
53	49482.0
54	42039.5
55	34597.0
56	28921.0
57	23245.0
58	20246.0
59	17247.0
60	14704.5
61	12162.0
62	9686.0
63	7210.0
64	5843.5
65	4477.0
66	3684.5
67	2892.0
68	2180.0
69	1468.0
70	1176.5
71	885.0
72	649.5
73	414.0
74	362.0
75	250.5
76	191.0
77	181.0
78	171.0
79	122.5
80	74.0
81	52.0
82	30.0
83	22.0
84	14.0
85	15.0
86	16.0
87	10.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	926780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.554876323680222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.16610785833329	19.1185763227753
2	7.809837499855058	3.679195128342516
3	2.7216515234461576	1.923244950927903
4	1.326565541036522	1.2498834901748486
5	0.7867903512046202	0.9266374707644875
6	0.5307103923750796	0.7500490593652082
7	0.3636443849421874	0.599591895917979
8	0.2769161852057619	0.5218181195637639
9	0.22470519933613015	0.4763612861685412
>10	2.6248389005831907	15.096220418589768
>50	1.2554622313434887	21.436844785296902
>100	0.9104637930450932	32.62899910008116
>500	0.0013836835760563727	0.19468583981722284
>1k	4.6122785868545757E-4	0.5216972113852143
>5k	4.6122785868545757E-4	0.8761949208291865
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8065	0.8702173115518247	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4802	0.5181380694447442	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.054813440082867565	0.0
2	0.0	0.0	0.0	0.2259435896329226	0.0
3	0.0	0.0	0.0	0.33675737499730246	0.0
4	0.0	0.0	0.0	0.5671248840069919	0.0
5	0.0	0.0	0.0	0.9257860549429207	0.0
6	0.0	0.0	0.0	1.4301128638943439	0.0
7	0.0	0.0	0.0	1.6596171691232007	0.0
8	0.0	0.0	0.0	2.2525302660825655	0.0
9	0.0	0.0	0.0	2.4493407281123893	0.0
10	0.0	0.0	0.0	2.8277476855348627	0.0
11	0.0	0.0	0.0	3.3161052245408835	0.0
12	0.0	0.0	0.0	3.661926239236928	0.0
13	0.0	0.0	0.0	3.812879000410022	0.0
14	0.0	0.0	0.0	3.8758928764107985	0.0
15	0.0	0.0	0.0	3.967608278124258	0.0
16	0.0	0.0	0.0	4.197004682880511	0.0
17	0.0	0.0	0.0	4.455318414294655	0.0
18	0.0	0.0	0.0	4.802002632771532	0.0
19	0.0	0.0	0.0	4.956731910485768	0.0
20	0.0	0.0	0.0	5.1273225576728025	0.0
21	0.0	0.0	0.0	5.392865620751419	0.0
22	0.0	0.0	0.0	5.649021342713481	0.0
23	0.0	0.0	0.0	5.9227648417100065	0.0
24	0.0	0.0	0.0	6.11558298625348	0.0
25	0.0	0.0	0.0	6.276678391851356	0.0
26	0.0	0.0	0.0	6.441550313990375	0.0
27	0.0	0.0	0.0	6.583439435464728	0.0
28	0.0	0.0	0.0	6.750577267528432	0.0
29	0.0	0.0	0.0	6.920088909989426	0.0
30	0.0	0.0	0.0	7.135566153779753	0.0
31	0.0	0.0	0.0	7.348777487645396	0.0
32	0.0	0.0	0.0	7.519583935777638	0.0
33	0.0	0.0	0.0	7.695569606594877	0.0
34	0.0	0.0	0.0	7.89076156153564	0.0
35	0.0	0.0	0.0	8.128358402209802	0.0
36	0.0	0.0	0.0	8.32344245667796	0.0
37	0.0	0.0	0.0	8.51669220311185	0.0
38	0.0	0.0	0.0	8.70573383111418	0.0
39	0.0	0.0	0.0	8.902652193616609	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	45	3.8380676E-10	45.000004	2
TATCGTG	45	3.8380676E-10	45.000004	1
CGGGTAG	20	7.032579E-4	45.0	6
CGTGGCT	20	7.032579E-4	45.0	22
CTGTCGT	40	6.8121153E-9	45.0	37
ACACGAA	20	7.032579E-4	45.0	36
GTCGACC	25	3.8902188E-5	45.0	15
CGAGTCA	20	7.032579E-4	45.0	14
GGTCGAC	25	3.8902188E-5	45.0	14
CCCTAGT	25	3.8902188E-5	45.0	35
AACCCGT	25	3.8902188E-5	45.0	37
CGGTTGC	20	7.032579E-4	45.0	31
CGTGACG	25	3.8902188E-5	45.0	19
GGCGTGT	25	3.8902188E-5	45.0	8
GACCGTA	25	3.8902188E-5	45.0	26
GAGTACG	55	1.8189894E-12	45.0	1
ATTACGC	25	3.8902188E-5	45.0	28
TTGTCGA	40	6.8121153E-9	45.0	1
CAACCCG	25	3.8902188E-5	45.0	1
ACTTCGT	25	3.8902188E-5	45.0	37
>>END_MODULE