FastQCFastQC Report
Thu 26 May 2016
SRR1548176_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548176_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2113321
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102110.48317316678346545No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC69880.33066439031268796No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG30090.14238253440911247No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG24020.11365996930896916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG1300.045.0000041
CGCAAAC351.2125747E-745.00000429
TCGACGA351.2125747E-745.0000042
ATGTTCG351.2125747E-745.0000041
AACCGAT253.8919206E-545.026
GGCGTAT207.034631E-445.08
GACCGAA207.034631E-445.09
CACGCTA207.034631E-445.041
TCGATTG750.045.01
TTATCCG406.8193913E-945.01
CGTTAGA406.8193913E-945.034
GCTAGCG453.8562575E-1045.01
ACGTAAT253.8919206E-545.029
CCGCGAA207.034631E-445.035
TATCGCT253.8919206E-545.038
TAATGCG900.045.01
CTATACG600.044.9999961
TACGACT302.166371E-644.99999615
TTGTGCG1400.043.392861
AACGGGA4050.042.7777754