##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548176_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2113321
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.230311911914942	31.0	31.0	33.0	30.0	34.0
2	31.624595127763364	31.0	31.0	34.0	30.0	34.0
3	31.721840647965927	31.0	31.0	34.0	30.0	34.0
4	35.512833592246515	37.0	35.0	37.0	33.0	37.0
5	35.375103924108075	37.0	35.0	37.0	33.0	37.0
6	35.42075908013974	37.0	35.0	37.0	33.0	37.0
7	35.818845315027865	37.0	35.0	37.0	35.0	37.0
8	35.814222259656724	37.0	35.0	37.0	35.0	37.0
9	37.55428967014476	39.0	37.0	39.0	35.0	39.0
10	36.923467376702355	39.0	37.0	39.0	32.0	39.0
11	36.56231779270636	39.0	35.0	39.0	32.0	39.0
12	35.629159980902095	37.0	35.0	39.0	31.0	39.0
13	35.208171877343766	37.0	35.0	39.0	30.0	39.0
14	36.11283425471095	38.0	35.0	40.0	30.0	41.0
15	36.45626812017672	38.0	35.0	40.0	31.0	41.0
16	36.65764689793931	38.0	35.0	40.0	32.0	41.0
17	36.55179359879545	38.0	35.0	40.0	31.0	41.0
18	36.5056803959266	38.0	35.0	40.0	31.0	41.0
19	36.41621457412291	37.0	35.0	40.0	31.0	41.0
20	36.27160710559352	37.0	35.0	40.0	31.0	41.0
21	36.0958160165919	37.0	34.0	40.0	31.0	41.0
22	35.97517698447136	37.0	34.0	40.0	30.0	41.0
23	35.998680749398694	37.0	34.0	40.0	30.0	41.0
24	35.8276248615331	37.0	34.0	40.0	30.0	41.0
25	35.726933106707406	36.0	34.0	40.0	30.0	41.0
26	35.62349023172533	36.0	34.0	40.0	30.0	41.0
27	35.585558464615644	36.0	34.0	40.0	30.0	41.0
28	35.63490023522219	36.0	34.0	40.0	30.0	41.0
29	35.747704205844734	36.0	34.0	40.0	30.0	41.0
30	35.70442824350868	36.0	34.0	40.0	30.0	41.0
31	35.54570744340306	36.0	34.0	40.0	30.0	41.0
32	35.33161124126434	36.0	34.0	40.0	29.0	41.0
33	35.205462397808944	36.0	34.0	40.0	29.0	41.0
34	35.09209438603979	36.0	34.0	40.0	27.0	41.0
35	34.94627271484077	36.0	34.0	40.0	27.0	41.0
36	34.726935945840694	36.0	34.0	40.0	25.0	41.0
37	34.64019900431595	36.0	33.0	40.0	25.0	41.0
38	34.615563371584344	36.0	33.0	40.0	26.0	41.0
39	34.623139125575335	36.0	33.0	40.0	25.0	41.0
40	34.481415743278	36.0	33.0	40.0	24.0	41.0
41	34.47359203831316	36.0	34.0	40.0	24.0	41.0
42	34.40956153845062	36.0	33.0	40.0	24.0	41.0
43	34.306962359244054	35.0	33.0	40.0	24.0	41.0
44	34.18510581213171	35.0	33.0	40.0	24.0	41.0
45	34.10510566071127	35.0	33.0	40.0	23.0	41.0
46	34.00308661107328	35.0	33.0	40.0	23.0	41.0
47	33.97255835720177	35.0	33.0	40.0	23.0	41.0
48	33.887010539335954	35.0	33.0	39.0	23.0	41.0
49	33.83743690617753	35.0	33.0	39.0	24.0	41.0
50	33.67856515881875	35.0	33.0	39.0	24.0	41.0
51	33.291119049117476	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	1.0
13	4.0
14	30.0
15	77.0
16	188.0
17	497.0
18	1080.0
19	2141.0
20	3759.0
21	5985.0
22	9037.0
23	13306.0
24	18411.0
25	25557.0
26	33829.0
27	41605.0
28	46515.0
29	53782.0
30	64033.0
31	78450.0
32	97199.0
33	127668.0
34	214032.0
35	301142.0
36	150698.0
37	180214.0
38	257403.0
39	386556.0
40	115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.32689212855028	24.586657682387106	29.55807470800697	13.528375481055646
2	30.393584315870616	27.081404102831513	29.41394137473673	13.111070206561143
3	28.930200381295602	25.386820080811194	31.52725023789571	14.15572929999749
4	25.840844812501274	28.634031460435967	30.947972409302704	14.577151317760057
5	23.51124131166065	33.11233835276326	28.794679085666587	14.581741249909502
6	21.953266919696535	41.283505913204856	26.25393870595144	10.50928846114717
7	88.0789998301252	4.2979746096310025	6.208758631556682	1.4142669286871232
8	89.02182867628723	3.4905724213217018	5.838535650760107	1.649063251630964
9	83.9082183918108	6.328853969652504	7.3596959477523765	2.4032316907843154
10	48.851830838760414	30.073188124284005	12.026805203752765	9.048175833202812
11	45.24920729032646	19.784263725198397	21.699211809280275	13.26731717519487
12	41.36621932967117	20.746635272161683	23.72304065496912	14.164104743198028
13	22.841016580065215	38.772765708569594	24.141008393897568	14.245209317467625
14	16.61863010872461	40.76550604475136	27.13246118313309	15.483402663390939
15	15.20493100669515	25.53464428735625	45.96343858789081	13.296986118057788
16	18.10094159855507	20.99695219041499	44.65828901525135	16.243817195778586
17	18.15389143438219	21.33859456277584	28.359913141448935	32.14760086139304
18	22.16189589749972	25.01328477784492	33.66554347399188	19.159275850663484
19	29.219082193381883	26.30258252295794	26.201414740117567	18.276920543542605
20	32.40785474615546	24.382713274509644	26.18248718486212	17.026944794472776
21	23.388874666934175	28.779300447021537	28.538636581948506	19.293188304095782
22	22.358411239939414	25.64972382330938	27.024337523736335	24.96752741301487
23	20.72340169808562	29.937808785319408	26.247692612717138	23.09109690387783
24	20.69524695964314	24.29157709595466	37.72853248512649	17.28464345927571
25	17.926808090204943	26.3108633283822	34.49215713088547	21.270171450527393
26	17.326331399725834	35.78353690707659	27.870730475871863	19.019401217325715
27	18.727585634174837	35.07484191942445	29.240517649708682	16.95705479669203
28	15.235735602873394	30.263741286818234	37.89589939247279	16.604623717835576
29	16.53894510109917	25.581868537718595	37.17598982833181	20.70319653285043
30	18.535470948332033	31.88427124890161	31.288147896131257	18.2921099066351
31	29.21785190229028	27.274701760877782	25.24746595524296	18.259980381588978
32	29.274303335839658	28.099943170015347	26.66826289049321	15.957490603651788
33	27.580713010470248	29.277757614673778	24.871280794540915	18.27024858031506
34	19.81913774575656	29.3957709216915	27.262730082178717	23.522361250373226
35	20.302121636987472	27.65003518159333	31.138998760718316	20.908844420700877
36	29.07267755348099	26.64526591085784	27.283645030735983	16.998411504925187
37	20.797408439134422	32.91658011253378	28.969096507345547	17.31691494098625
38	19.973491958864745	34.25750276460604	23.730848271511995	22.038157005017222
39	20.033918179017764	32.604701320812126	27.945115768025776	19.416264732144338
40	24.329432206465558	28.081583441417564	25.360226865677294	22.22875748643959
41	17.31029029664684	26.00054605996912	28.198461095119953	28.490702548264085
42	21.252284910810992	26.59752115272597	25.395668712893123	26.75452522356992
43	22.603523080497474	26.297377445262693	26.194932052442578	24.904167421797258
44	18.353056634557646	31.533780244458843	29.115832379463413	20.997330741520102
45	18.30181027870352	37.153087486472714	23.990061140735364	20.555041094088402
46	21.82290338287463	34.533277244677926	24.90435669734981	18.73946267509763
47	21.457223015339363	28.068097558298057	27.114480005640413	23.36019942072217
48	22.203820432390536	24.937243324606154	31.75674684536802	21.102189397635286
49	20.531381650019092	24.59427602337742	32.65897608550713	22.21536624109636
50	18.80367440630174	33.3739644852817	27.997403139418953	19.824957968997612
51	18.539161821606847	33.09710167078262	25.911823144709206	22.45191336290133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1213.0
1	2234.5
2	3256.0
3	10446.5
4	17637.0
5	12981.5
6	8326.0
7	8421.0
8	8516.0
9	9069.5
10	9623.0
11	10048.0
12	10473.0
13	10486.5
14	10500.0
15	9992.5
16	9485.0
17	9176.0
18	8867.0
19	8792.0
20	8717.0
21	8452.5
22	8188.0
23	9125.5
24	10063.0
25	11435.0
26	16034.0
27	19261.0
28	26786.5
29	34312.0
30	37697.0
31	41082.0
32	45374.0
33	49666.0
34	56502.5
35	63339.0
36	68302.5
37	73266.0
38	83992.5
39	94719.0
40	127967.0
41	161215.0
42	185911.0
43	210607.0
44	211685.0
45	212763.0
46	205107.0
47	197451.0
48	182090.0
49	166729.0
50	154690.0
51	142651.0
52	129107.0
53	115563.0
54	101534.5
55	87506.0
56	82255.5
57	77005.0
58	72464.0
59	67923.0
60	65828.0
61	63733.0
62	52230.0
63	40727.0
64	33458.0
65	26189.0
66	19777.0
67	13365.0
68	12128.0
69	10891.0
70	8634.0
71	6377.0
72	5052.5
73	3728.0
74	3090.0
75	2070.5
76	1689.0
77	1227.5
78	766.0
79	570.0
80	374.0
81	283.5
82	193.0
83	118.0
84	43.0
85	32.0
86	21.0
87	19.5
88	18.0
89	14.0
90	10.0
91	9.5
92	9.0
93	7.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2113321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.808657491745596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.54520817789685	14.700193400250026
2	7.664695180863311	2.729958625092555
3	2.244122976227405	1.198944523589718
4	1.0277869055869067	0.7321401990439315
5	0.5593660033857711	0.4980778783411902
6	0.35638363215931695	0.3808028424473716
7	0.25947684044824937	0.3234653925008234
8	0.2012402592657792	0.2867055080651476
9	0.1572753241306202	0.25207761414109375
>10	2.0427657403196986	9.355591094000697
>50	1.2532845702211142	16.494751585640646
>100	1.6774498566936624	50.69841904029872
>500	0.008809014205936796	1.032914334350665
>1k	0.001601638946533963	0.4983450371409252
>5k	2.6693982442232717E-4	0.33219833249458985
>10k+	2.6693982442232717E-4	0.48541459260192565
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10211	0.48317316678346545	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	6988	0.33066439031268796	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3009	0.14238253440911247	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2402	0.11365996930896916	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.029810899527331628	0.0
2	0.0	0.0	0.0	0.10722460052211662	0.0
3	0.0	0.0	0.0	0.15932269636273902	0.0
4	0.0	0.0	0.0	0.2907745676118299	0.0
5	0.0	0.0	0.0	0.49145397220772424	0.0
6	0.0	0.0	0.0	0.7420548037898644	0.0
7	0.0	0.0	0.0	0.8646107240688944	0.0
8	0.0	0.0	0.0	1.1837293056757587	0.0
9	0.0	0.0	0.0	1.287972816245142	0.0
10	0.0	0.0	0.0	1.4846774342373923	0.0
11	0.0	0.0	0.0	1.7449313189998112	0.0
12	0.0	0.0	0.0	1.9536076156911326	0.0
13	0.0	0.0	0.0	2.0327721155470466	0.0
14	0.0	0.0	0.0	2.0625356961862398	0.0
15	0.0	0.0	0.0	2.1175675630914563	0.0
16	0.0	0.0	0.0	2.242915297770665	0.0
17	0.0	0.0	0.0	2.385723702173025	0.0
18	0.0	0.0	0.0	2.575472443608898	0.0
19	0.0	0.0	0.0	2.664384634421368	0.0
20	0.0	0.0	0.0	2.767350535011009	0.0
21	0.0	0.0	0.0	2.891089427493504	0.0
22	0.0	0.0	0.0	3.0271312308920413	0.0
23	0.0	0.0	0.0	3.1741983352268774	0.0
24	0.0	0.0	0.0	3.2844513445898658	0.0
25	0.0	0.0	0.0	3.3740733187244154	0.0
26	0.0	0.0	0.0	3.4654460917200938	0.0
27	0.0	0.0	0.0	3.5519923381256326	0.0
28	0.0	0.0	0.0	3.6451632288705786	0.0
29	0.0	0.0	0.0	3.745668547277011	0.0
30	0.0	0.0	0.0	3.8739027341326757	0.0
31	0.0	0.0	0.0	3.999155831035607	0.0
32	0.0	0.0	0.0	4.109882029279982	0.0
33	0.0	0.0	0.0	4.218526196446257	0.0
34	0.0	0.0	0.0	4.3385742156539395	0.0
35	0.0	0.0	0.0	4.486114508870163	0.0
36	0.0	0.0	0.0	4.623244646695888	0.0
37	0.0	0.0	0.0	4.758671304548622	0.0
38	0.0	0.0	0.0	4.8994923156491605	0.0
39	0.0	0.0	0.0	5.041922168946412	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGTAG	130	0.0	45.000004	1
CGCAAAC	35	1.2125747E-7	45.000004	29
TCGACGA	35	1.2125747E-7	45.000004	2
ATGTTCG	35	1.2125747E-7	45.000004	1
AACCGAT	25	3.8919206E-5	45.0	26
GGCGTAT	20	7.034631E-4	45.0	8
GACCGAA	20	7.034631E-4	45.0	9
CACGCTA	20	7.034631E-4	45.0	41
TCGATTG	75	0.0	45.0	1
TTATCCG	40	6.8193913E-9	45.0	1
CGTTAGA	40	6.8193913E-9	45.0	34
GCTAGCG	45	3.8562575E-10	45.0	1
ACGTAAT	25	3.8919206E-5	45.0	29
CCGCGAA	20	7.034631E-4	45.0	35
TATCGCT	25	3.8919206E-5	45.0	38
TAATGCG	90	0.0	45.0	1
CTATACG	60	0.0	44.999996	1
TACGACT	30	2.166371E-6	44.999996	15
TTGTGCG	140	0.0	43.39286	1
AACGGGA	405	0.0	42.777775	4
>>END_MODULE