FastQCFastQC Report
Thu 26 May 2016
SRR1548175_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548175_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2686349
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357711.331584243149345No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT217550.042.9625361
TCTATCG501.0822987E-940.50000442
TACGGGA10550.039.668254
TATCGCG700.038.571431
CGTTAGG3800.038.4868432
ACGGGAC9650.038.2383425
GGGCGAT43250.037.9248547
GACCGAT15150.037.8712849
GGCGATA10350.037.8260888
TAGGGAC28700.037.7874575
TAGGGCG7450.037.751685
AGGGCGA22350.037.6510056
AGGGATT49850.037.417256
CGTAAGG4300.037.151162
ATAGGGC8800.036.8181844
CGCGTAA552.752131E-936.81818431
TAAGGGA38400.036.796884
GTTTTTT262950.036.682832
ACGTAAG1350.036.6666641
GCGATAC1600.036.56259
Produced by FastQC (version 0.11.5)