##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548175_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2686349
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145184412003058	31.0	31.0	33.0	30.0	34.0
2	31.529878656868487	31.0	31.0	34.0	30.0	34.0
3	31.512545093731305	31.0	31.0	34.0	30.0	34.0
4	35.3899917694983	37.0	35.0	37.0	33.0	37.0
5	35.28294164309998	37.0	35.0	37.0	33.0	37.0
6	35.341016748010034	37.0	35.0	37.0	33.0	37.0
7	35.775853770303115	37.0	35.0	37.0	35.0	37.0
8	35.773462420556676	37.0	35.0	37.0	35.0	37.0
9	37.49527220774367	39.0	37.0	39.0	35.0	39.0
10	36.84118854251626	39.0	37.0	39.0	32.0	39.0
11	36.5436702379326	39.0	35.0	39.0	32.0	39.0
12	36.13667099844436	38.0	35.0	39.0	31.0	39.0
13	35.988103928417345	38.0	35.0	39.0	31.0	39.0
14	36.97138272056237	39.0	35.0	41.0	31.0	41.0
15	37.12118827449449	39.0	35.0	41.0	31.0	41.0
16	37.17319752571241	39.0	35.0	41.0	32.0	41.0
17	37.01656821209753	39.0	35.0	41.0	31.0	41.0
18	36.868192479830434	39.0	35.0	40.0	31.0	41.0
19	36.73592857815571	38.0	35.0	40.0	31.0	41.0
20	36.55642584042505	38.0	35.0	40.0	31.0	41.0
21	36.42194964243291	38.0	35.0	40.0	30.0	41.0
22	36.36225002782587	38.0	35.0	40.0	30.0	41.0
23	36.28679780624186	38.0	35.0	40.0	30.0	41.0
24	36.12412013480006	38.0	35.0	40.0	30.0	41.0
25	35.990620354987385	38.0	34.0	40.0	30.0	41.0
26	35.94658996280826	38.0	34.0	40.0	30.0	41.0
27	35.922052570235664	38.0	34.0	40.0	30.0	41.0
28	35.9018749983714	38.0	34.0	40.0	30.0	41.0
29	35.883780178971534	38.0	34.0	40.0	30.0	41.0
30	35.75354430865089	38.0	34.0	40.0	29.0	41.0
31	35.5803687458331	38.0	34.0	40.0	29.0	41.0
32	35.33059367937673	38.0	34.0	40.0	27.0	41.0
33	35.05843432852544	38.0	34.0	40.0	25.0	41.0
34	34.74738650860331	38.0	34.0	40.0	24.0	41.0
35	34.46236285754382	38.0	33.0	40.0	23.0	41.0
36	34.28650856608728	37.0	33.0	40.0	22.0	41.0
37	34.20973708181625	37.0	33.0	40.0	22.0	41.0
38	34.10431667664923	37.0	33.0	40.0	21.0	41.0
39	34.04925421082667	37.0	33.0	40.0	21.0	41.0
40	33.930974344733315	37.0	33.0	40.0	21.0	41.0
41	33.850673162720106	37.0	33.0	40.0	20.0	41.0
42	33.79169348435367	37.0	33.0	40.0	20.0	41.0
43	33.68970375777682	37.0	33.0	40.0	19.0	41.0
44	33.53785416563522	37.0	32.0	40.0	18.0	41.0
45	33.44982651174512	36.0	32.0	40.0	19.0	41.0
46	33.33739398715506	36.0	32.0	40.0	19.0	41.0
47	33.303471738035526	36.0	32.0	40.0	19.0	41.0
48	33.178439212477606	36.0	32.0	40.0	18.0	41.0
49	33.06449273716855	36.0	31.0	40.0	18.0	41.0
50	32.94014366711101	35.0	31.0	40.0	18.0	41.0
51	32.60920193169242	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	6.0
13	10.0
14	34.0
15	136.0
16	304.0
17	808.0
18	1815.0
19	3402.0
20	5865.0
21	9280.0
22	13888.0
23	20241.0
24	29755.0
25	43255.0
26	59380.0
27	69006.0
28	71489.0
29	74893.0
30	82838.0
31	96868.0
32	115762.0
33	144347.0
34	209802.0
35	258644.0
36	217576.0
37	265662.0
38	373060.0
39	518141.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.933248062705182	23.463928179101075	27.212063659636183	18.39076009855756
2	34.66377600229903	24.808876285248118	27.737497994489917	12.78984971796293
3	27.420562257547328	24.26434539964837	35.29969486466576	13.015397478138544
4	24.669095489826525	27.064316661759136	34.385405619299654	13.881182229114684
5	22.27022624387226	30.781406287865053	33.21295929903375	13.735408169228943
6	20.988077126240857	38.858130496074786	30.403532824662765	9.750259553021593
7	84.46925548393006	3.072162254420405	10.940871792905538	1.517710468744009
8	85.02748525973357	2.525211727887925	10.835971052160385	1.611331960218125
9	80.06908260989172	5.184062085752819	12.223207036762537	2.52364826759293
10	37.992569096569355	35.430243799297855	16.97110092545682	9.606086178675966
11	30.013412255816352	22.79346428926398	31.09975658412217	16.093366870797503
12	28.731039786714234	21.804650103169767	32.38279910763642	17.081511002479573
13	22.910128207466716	26.25913460983662	33.23574114904653	17.59499603365013
14	18.56988797806986	28.830431191181788	34.50813725245677	18.091543578291578
15	18.532513831970455	25.175917202120797	39.80413565028223	16.487433315626525
16	23.06554360583826	24.5054533122837	36.665154080873336	15.76384900100471
17	22.05986638370517	23.87649557075421	32.76729866446988	21.296339381070737
18	22.90074744569674	24.49942282257443	34.46934854704284	18.13048118468598
19	24.352383104354647	26.453301488376972	31.37883424677881	17.815481160489572
20	26.2453240438975	26.26028859243531	30.984321099008355	16.51006626465884
21	24.336897402385173	25.927681027297645	33.01585162612899	16.71956994418819
22	23.25453617530708	23.114085325473347	33.69569627773606	19.935682221483507
23	20.891254263686513	26.25570988728568	33.28316611132805	19.569869737699754
24	21.009034939242817	25.136942370481275	35.787866729155446	18.066155961120465
25	20.796329888633235	26.212975305889145	33.58312713649641	19.40756766898121
26	19.735224276518053	29.040046546446497	31.73936074575567	19.485368431279777
27	18.698203397994824	28.096312132191315	33.6999027304345	19.50558173937936
28	17.937096036293124	27.815335982033606	35.49293111207814	18.754636869595124
29	19.970338924689234	25.893061549337038	35.23492293815882	18.90167658781491
30	21.04559012994961	26.119093237699197	34.35313133178154	18.482185300569657
31	24.08231395101679	25.53748600796099	32.09854713590825	18.28165290511397
32	25.021097407671157	25.788681962023553	31.377196335993574	17.81302429431172
33	23.915284276168137	26.5711193891784	31.092683787549568	18.420912547103896
34	20.613181682648086	27.28822651114952	32.70863167816244	19.389960128039956
35	19.749891023094914	28.552879763575024	32.186808192085245	19.51042102124482
36	23.037885248714893	28.653871853582686	29.959584551374373	18.348658346328044
37	21.463220154938913	29.554015505803598	29.900954790312056	19.08180954894543
38	20.744996275614223	30.55731031224908	28.61150952463734	20.086183887499352
39	20.522947688479793	29.133593587430372	29.647376420561883	20.696082303527948
40	22.394149084873185	27.389851430324207	28.970286437093616	21.245713047708993
41	19.300954566960584	27.386426707773264	29.62839154555123	23.684227179714924
42	20.738816884924482	27.782987244025254	29.07228360871949	22.40591226233077
43	20.978808040206243	27.218578077531998	29.28446750589741	22.51814637636435
44	20.293230700850856	28.49250041599212	29.89213240721887	21.322136475938162
45	20.10662054706965	29.757898173319997	28.592226847665735	21.543254431944618
46	21.01860182723838	29.55863143619835	28.92870583829577	20.4940608982675
47	20.29598536898966	28.265054168315434	30.26017840570976	21.17878205698515
48	20.707994381966007	27.146323876756146	31.455257675007974	20.69042406626987
49	21.633488426112915	26.380749485640177	31.047343438994712	20.9384186492522
50	19.592539911977187	28.498791482417214	31.026348400747633	20.88232020485797
51	19.17304862473193	28.814387110535527	30.323796349618014	21.68876791511453
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1664.0
1	2543.5
2	3423.0
3	31534.0
4	59645.0
5	42125.0
6	24605.0
7	24100.0
8	23595.0
9	24193.5
10	24792.0
11	24881.0
12	24970.0
13	24420.0
14	23870.0
15	22321.0
16	20772.0
17	19607.5
18	18443.0
19	17645.5
20	16848.0
21	17356.0
22	17864.0
23	19513.0
24	21162.0
25	22121.5
26	26783.5
27	30486.0
28	35381.0
29	40276.0
30	48987.0
31	57698.0
32	64153.0
33	70608.0
34	81518.5
35	92429.0
36	96745.0
37	101061.0
38	112575.5
39	124090.0
40	139165.5
41	154241.0
42	168864.5
43	183488.0
44	188742.5
45	193997.0
46	197862.0
47	201727.0
48	195924.5
49	190122.0
50	186371.5
51	182621.0
52	170007.5
53	157394.0
54	145301.0
55	133208.0
56	123767.5
57	114327.0
58	106657.0
59	98987.0
60	89904.5
61	80822.0
62	71638.0
63	62454.0
64	53386.0
65	44318.0
66	35317.5
67	26317.0
68	21453.5
69	16590.0
70	13468.5
71	10347.0
72	8121.0
73	5895.0
74	4853.5
75	3026.0
76	2240.0
77	1647.0
78	1054.0
79	818.5
80	583.0
81	416.5
82	250.0
83	160.5
84	71.0
85	57.0
86	43.0
87	33.5
88	24.0
89	18.5
90	13.0
91	9.5
92	6.0
93	7.0
94	8.0
95	8.0
96	8.0
97	4.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2686349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.831191636074394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.75922965235979	19.80516716243413
2	6.015425505533744	2.9874036700087614
3	2.203969215332101	1.6418154583775972
4	1.2359313007295358	1.2275858790975118
5	0.8856372549821039	1.099571419925375
6	0.6474128694995278	0.964561981812216
7	0.49431690186651567	0.8592134403438623
8	0.4271598902642229	0.8485511275516342
9	0.36350008416251695	0.8123526224611766
>10	6.371922790218281	40.00736237848747
>50	1.4977295655360674	24.641079908600595
>100	0.09518869302356218	3.2282831307517785
>500	0.0018185481120293568	0.3202740129764588
>1k	6.061827040097857E-4	0.21069371385914615
>5k	0.0	0.0
>10k+	1.5154567600244643E-4	1.3460840933122908
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35771	1.331584243149345	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.116757353567984E-4	0.0	0.0	0.025610968641825764	0.0
2	1.116757353567984E-4	0.0	0.0	0.0813743858299871	0.0
3	1.116757353567984E-4	0.0	0.0	0.15359136136071672	0.0
4	1.116757353567984E-4	0.0	0.0	0.2349285219455849	0.0
5	1.116757353567984E-4	0.0	0.0	0.42604293038618585	0.0
6	1.116757353567984E-4	0.0	0.0	0.7950567852501667	0.0
7	1.116757353567984E-4	0.0	0.0	1.031362641265152	0.0
8	1.116757353567984E-4	0.0	0.0	1.4690198481284449	0.0
9	1.116757353567984E-4	0.0	0.0	1.72661854435146	0.0
10	1.116757353567984E-4	0.0	0.0	2.0349552496715804	0.0
11	1.116757353567984E-4	0.0	0.0	2.2823542287320078	0.0
12	1.116757353567984E-4	0.0	0.0	2.476037179085815	0.0
13	1.116757353567984E-4	0.0	0.0	2.5843626423819095	0.0
14	1.116757353567984E-4	0.0	0.0	2.6529687691361024	0.0
15	1.116757353567984E-4	0.0	0.0	2.7145393245628173	0.0
16	1.116757353567984E-4	0.0	0.0	2.8057039498590837	0.0
17	1.116757353567984E-4	0.0	0.0	2.9145133413417246	0.0
18	1.116757353567984E-4	0.0	0.0	3.046439610043222	0.0
19	1.116757353567984E-4	0.0	0.0	3.1375670100943696	0.0
20	1.116757353567984E-4	0.0	0.0	3.2324913851476484	0.0
21	1.116757353567984E-4	0.0	0.0	3.344018219523971	0.0
22	1.116757353567984E-4	0.0	0.0	3.471403008320959	0.0
23	1.116757353567984E-4	0.0	0.0	3.6064934228575662	0.0
24	1.116757353567984E-4	0.0	0.0	3.7190253388521	0.0
25	1.116757353567984E-4	0.0	0.0	3.8190495724866724	0.0
26	1.116757353567984E-4	0.0	0.0	3.916840291414109	0.0
27	1.116757353567984E-4	0.0	0.0	4.029037180202573	0.0
28	1.116757353567984E-4	0.0	0.0	4.136394787125575	0.0
29	1.116757353567984E-4	0.0	0.0	4.256744004595085	0.0
30	1.116757353567984E-4	0.0	0.0	4.410893744632585	0.0
31	1.116757353567984E-4	0.0	0.0	4.545053528041219	0.0
32	1.116757353567984E-4	0.0	0.0	4.693507805575523	0.0
33	1.116757353567984E-4	0.0	0.0	4.8286726706023675	0.0
34	1.116757353567984E-4	0.0	0.0	4.973441648869898	0.0
35	1.116757353567984E-4	0.0	0.0	5.135706492343325	0.0
36	1.116757353567984E-4	0.0	0.0	5.286840987526193	0.0
37	1.116757353567984E-4	0.0	0.0	5.448696353303313	0.0
38	1.116757353567984E-4	0.0	0.0	5.645096746550802	0.0
39	1.116757353567984E-4	0.0	0.0	5.899419621203351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	21755	0.0	42.962536	1
TCTATCG	50	1.0822987E-9	40.500004	42
TACGGGA	1055	0.0	39.66825	4
TATCGCG	70	0.0	38.57143	1
CGTTAGG	380	0.0	38.486843	2
ACGGGAC	965	0.0	38.238342	5
GGGCGAT	4325	0.0	37.924854	7
GACCGAT	1515	0.0	37.871284	9
GGCGATA	1035	0.0	37.826088	8
TAGGGAC	2870	0.0	37.787457	5
TAGGGCG	745	0.0	37.75168	5
AGGGCGA	2235	0.0	37.651005	6
AGGGATT	4985	0.0	37.41725	6
CGTAAGG	430	0.0	37.15116	2
ATAGGGC	880	0.0	36.818184	4
CGCGTAA	55	2.752131E-9	36.818184	31
TAAGGGA	3840	0.0	36.79688	4
GTTTTTT	26295	0.0	36.68283	2
ACGTAAG	135	0.0	36.666664	1
GCGATAC	160	0.0	36.5625	9
>>END_MODULE