FastQCFastQC Report
Thu 26 May 2016
SRR1548174_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548174_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2672622
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437251.6360338274548365No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT206500.043.539951
CGCCGGT6050.040.90909228
CATACGA4100.040.60975618
CGTAAGG3800.039.671052
CGGTCTA2050.039.51219631
ACGCCGG6400.038.67187527
TAAGGGA34400.038.655524
AAGGGAT48250.038.5181355
TCGTCCC6350.038.26771538
CTCGTCC6550.037.7862637
CACGCCG6550.037.7862626
GTTTTTT245500.037.5763782
GCGTTAG1800.037.51
CGGGCGA3900.037.56
AGGGATG55100.037.4863856
GGGCGAT34650.037.4675337
TTAGGGA40500.037.388894
TAGGGAT47350.037.3495255
ATAGGGA41700.037.3381274
AGGGCGA19000.037.302636