##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548174_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2672622
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09251476639794	31.0	31.0	33.0	30.0	34.0
2	31.47332207846826	31.0	31.0	34.0	30.0	34.0
3	31.46364094885098	31.0	31.0	34.0	30.0	34.0
4	35.343553259682814	37.0	35.0	37.0	33.0	37.0
5	35.21766751901316	37.0	35.0	37.0	32.0	37.0
6	35.27758208979796	37.0	35.0	37.0	32.0	37.0
7	35.74259472532966	37.0	35.0	37.0	35.0	37.0
8	35.74857349823507	37.0	35.0	37.0	35.0	37.0
9	37.47748091574491	39.0	37.0	39.0	35.0	39.0
10	36.77779648599765	39.0	37.0	39.0	32.0	39.0
11	36.432801945056205	39.0	35.0	39.0	32.0	39.0
12	36.0741410495012	38.0	35.0	39.0	31.0	39.0
13	35.90982301275676	38.0	35.0	39.0	31.0	39.0
14	36.87587807029951	39.0	35.0	41.0	31.0	41.0
15	37.0726821825159	39.0	35.0	41.0	31.0	41.0
16	37.135963484548135	39.0	35.0	41.0	32.0	41.0
17	37.00194565486627	39.0	35.0	41.0	31.0	41.0
18	36.854947688075605	38.0	35.0	40.0	31.0	41.0
19	36.73472268057361	38.0	35.0	40.0	31.0	41.0
20	36.5491865291837	38.0	35.0	40.0	31.0	41.0
21	36.40103052358321	38.0	35.0	40.0	30.0	41.0
22	36.34908004199621	38.0	35.0	40.0	30.0	41.0
23	36.269255809463516	38.0	35.0	40.0	30.0	41.0
24	36.082433280875485	38.0	34.0	40.0	30.0	41.0
25	35.96291881156407	38.0	34.0	40.0	30.0	41.0
26	35.89296353917614	38.0	34.0	40.0	30.0	41.0
27	35.85399431719113	38.0	34.0	40.0	30.0	41.0
28	35.82883887059225	38.0	34.0	40.0	30.0	41.0
29	35.834142650924825	38.0	34.0	40.0	30.0	41.0
30	35.71527735684283	38.0	34.0	40.0	29.0	41.0
31	35.57871259010814	38.0	34.0	40.0	29.0	41.0
32	35.35992594538247	38.0	34.0	40.0	27.0	41.0
33	35.13948960982885	38.0	34.0	40.0	26.0	41.0
34	34.85247408724466	38.0	34.0	40.0	24.0	41.0
35	34.61292244095873	38.0	33.0	40.0	23.0	41.0
36	34.41998382113146	37.0	33.0	40.0	23.0	41.0
37	34.33195191837828	37.0	33.0	40.0	23.0	41.0
38	34.22208901969676	37.0	33.0	40.0	22.0	41.0
39	34.17470745956592	37.0	33.0	40.0	22.0	41.0
40	34.05124480753358	37.0	33.0	40.0	21.0	41.0
41	33.96885717471457	37.0	33.0	40.0	21.0	41.0
42	33.92126159254844	37.0	33.0	40.0	21.0	41.0
43	33.82380823026975	37.0	33.0	40.0	21.0	41.0
44	33.67644358236967	37.0	33.0	40.0	20.0	41.0
45	33.59915169447831	36.0	33.0	40.0	20.0	41.0
46	33.50329676250514	36.0	32.0	40.0	20.0	41.0
47	33.465333668584634	36.0	32.0	40.0	20.0	41.0
48	33.33800477583437	36.0	32.0	40.0	20.0	41.0
49	33.240020848440224	36.0	32.0	40.0	19.0	41.0
50	33.10663685324749	36.0	32.0	40.0	18.0	41.0
51	32.77724833515551	35.0	31.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	11.0
13	18.0
14	41.0
15	104.0
16	312.0
17	815.0
18	1852.0
19	3575.0
20	5938.0
21	9295.0
22	13951.0
23	20230.0
24	28595.0
25	41316.0
26	56106.0
27	66788.0
28	70415.0
29	74100.0
30	83141.0
31	96830.0
32	116300.0
33	144694.0
34	206676.0
35	262401.0
36	219608.0
37	260222.0
38	367938.0
39	521281.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.718119509605174	23.62425363556837	28.65104006477534	18.00658679005112
2	33.56288319111344	24.921631266973034	28.980491816650467	12.534993725263055
3	26.588795572288188	24.935138601717714	35.44500494271169	13.031060883282407
4	24.357017191357404	26.975494476959334	34.8877244892843	13.779763842398962
5	22.378622940318536	31.440098899133513	33.3105093050944	12.870768855453557
6	20.925480670293066	39.426338629256215	29.52280569418346	10.125375006267253
7	85.10500175483102	3.015091546803102	10.449326541501193	1.4305801568646819
8	86.1294638748016	2.339949308207446	10.026633021804056	1.5039537951868989
9	81.36582726625763	5.0802545215896595	11.433416322996667	2.120501889156042
10	39.1464262435915	35.61401500099902	16.233945541120292	9.00561321428919
11	28.215924287085866	23.282791206537997	31.970888513227834	16.5303959931483
12	28.77219449664038	22.340757503305742	31.44720802268334	17.439839977370536
13	23.014627582950375	27.310670944114058	31.920900149740593	17.753801323194978
14	17.340798661389453	31.005357285841395	33.048519394063206	18.605324658705946
15	16.951069025099695	26.91319610479896	39.84652524749104	16.289209622610308
16	21.107549066048247	25.500164258170443	37.38111861684892	16.01116805893239
17	20.99687123730928	24.61799685851572	32.410082682848525	21.975049221326472
18	21.312142158524473	25.945345058148888	34.560929304630434	18.1815834786962
19	23.951984231215636	27.28556451305123	30.620753701795465	18.141697553937668
20	25.14392233544437	27.219112916080164	30.95622950046808	16.680735248007387
21	23.039434682495315	26.351088930645634	33.440082435900024	17.169393950959023
22	22.38023184722718	23.87150146934359	33.330976097629964	20.417290585799265
23	19.69081299188587	27.102186541905287	32.86678774626565	20.34021271994319
24	19.6542197138241	26.897780531627742	35.69943673291621	17.74856302163194
25	19.752886865407827	28.056006423654374	33.48176434976588	18.709342361171913
26	19.000853843154776	30.942198335567095	30.779287156956727	19.277660664321406
27	17.98989905792888	30.456345865595658	33.31140729964806	18.2423477768274
28	16.951742521014943	28.587394700784476	36.08800645957416	18.372856318626425
29	18.006437124292173	26.69228944459785	35.573006583048404	19.728266848061565
30	19.5772166808475	27.774522547520746	33.88866813189445	18.759592639737306
31	22.92486554402381	27.498950468865406	31.043297555733655	18.532886431377126
32	23.009389281387342	27.648092397652942	31.190755744733075	18.15176257622664
33	21.92479894276108	28.82689733153435	30.496082124595247	18.752221601109323
34	19.270776039409988	28.746002988825207	31.612064856159982	20.371156115604823
35	18.58343604146041	29.610098248087457	31.65116503568406	20.15530067476807
36	22.29226579740794	28.490860286265697	30.327745562223164	18.8891283541032
37	19.565580168089614	30.424092894543257	30.712498812028038	19.297828125339088
38	19.224753818534758	30.528784092924475	29.40868555298879	20.837776535551978
39	19.155683070782175	29.98860295245643	29.919719286902524	20.935994689858873
40	21.3124789064821	28.4035677323617	29.270581473923365	21.013371887232836
41	18.736843444377843	27.811265491341462	30.332946447346465	23.118944616934233
42	20.214343816671416	28.416326738311664	28.784953502590337	22.584375942426576
43	20.175580385104965	28.996169304899833	28.989284679988415	21.838965630006786
44	19.466613684988	30.085474114932826	29.898990579288803	20.54892162079037
45	18.531389773787687	31.399951059296825	28.920139099356362	21.148520067559122
46	20.65076916975165	30.702433789739064	28.90756717560508	19.739229864904203
47	19.423846694369796	28.696463622614793	30.588351064984128	21.291338618031283
48	19.787459655723854	28.138135508874807	31.55448095540634	20.519923879995
49	20.205139372496372	26.95016354725809	31.195058635302708	21.64963844494283
50	18.637427963999396	29.580950841533145	31.307794368227153	20.4738268262403
51	18.685246173981955	30.00996773954566	30.034961921289284	21.269824165183103
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1671.0
1	2547.5
2	3424.0
3	33636.0
4	63848.0
5	43479.5
6	23111.0
7	23037.5
8	22964.0
9	24237.5
10	25511.0
11	26256.0
12	27001.0
13	26139.5
14	25278.0
15	23926.0
16	22574.0
17	20683.5
18	18793.0
19	18080.0
20	17367.0
21	17230.0
22	17093.0
23	18312.5
24	19532.0
25	22011.0
26	29479.0
27	34468.0
28	38672.5
29	42877.0
30	51939.5
31	61002.0
32	68060.5
33	75119.0
34	83990.0
35	92861.0
36	97801.0
37	102741.0
38	113930.0
39	125119.0
40	143768.0
41	162417.0
42	175929.5
43	189442.0
44	201996.0
45	214550.0
46	214122.0
47	213694.0
48	210139.0
49	206584.0
50	196030.5
51	185477.0
52	170726.0
53	155975.0
54	142213.0
55	128451.0
56	114345.5
57	100240.0
58	91861.5
59	83483.0
60	73662.0
61	63841.0
62	55310.0
63	46779.0
64	39005.5
65	31232.0
66	24973.0
67	18714.0
68	15028.5
69	11343.0
70	8970.0
71	6597.0
72	5018.0
73	3439.0
74	2490.0
75	1258.0
76	975.0
77	723.5
78	472.0
79	377.5
80	283.0
81	192.5
82	102.0
83	79.0
84	56.0
85	48.5
86	41.0
87	29.0
88	17.0
89	10.5
90	4.0
91	6.5
92	9.0
93	7.5
94	6.0
95	10.0
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2672622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.605240786255596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59008816364386	19.82938524252024
2	6.436244658635055	3.1673069916993395
3	2.3332086309585005	1.72227480507911
4	1.3045513062669676	1.28395196034892
5	0.8946274447012524	1.100626184543344
6	0.6318539248696369	0.9328150777894841
7	0.4715126896397708	0.8121178283653095
8	0.3826463121214585	0.7532083716576959
9	0.32600386070665915	0.7219263140942649
>10	4.702864469941576	28.789997462223702
>50	1.5318702967463969	26.342033873855996
>100	0.3922220924133621	12.385876295571098
>500	0.0012299463228578218	0.19871524734217214
>1k	9.224597421433663E-4	0.30569539573045745
>5k	0.0	0.0
>10k+	1.5374329035722773E-4	1.6540689491788458
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43725	1.6360338274548365	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.12249319207879E-4	0.0	0.0	0.03023248330665541	0.0
2	1.12249319207879E-4	0.0	0.0	0.09316693494253957	0.0
3	1.12249319207879E-4	0.0	0.0	0.16901005828732982	0.0
4	1.12249319207879E-4	0.0	0.0	0.258846930093369	0.0
5	1.12249319207879E-4	0.0	0.0	0.46748099806108007	0.0
6	1.12249319207879E-4	0.0	0.0	0.862710850992022	0.0
7	1.12249319207879E-4	0.0	0.0	1.1318847184525158	0.0
8	1.12249319207879E-4	0.0	0.0	1.6112267279098953	0.0
9	1.12249319207879E-4	0.0	0.0	1.8732540553808208	0.0
10	1.12249319207879E-4	0.0	0.0	2.175391806248695	0.0
11	1.12249319207879E-4	0.0	0.0	2.442582602403183	0.0
12	1.12249319207879E-4	0.0	0.0	2.6575400486862715	0.0
13	1.12249319207879E-4	0.0	0.0	2.7655987266437227	0.0
14	1.12249319207879E-4	0.0	0.0	2.8380743704122766	0.0
15	1.12249319207879E-4	0.0	0.0	2.898913501422947	0.0
16	1.12249319207879E-4	0.0	0.0	3.004914275194921	0.0
17	1.12249319207879E-4	0.0	0.0	3.129174271558043	0.0
18	1.12249319207879E-4	0.0	0.0	3.294068521474417	0.0
19	1.12249319207879E-4	0.0	0.0	3.397263062266194	0.0
20	1.12249319207879E-4	0.0	0.0	3.5062945676567807	0.0
21	1.12249319207879E-4	0.0	0.0	3.6345581230716504	0.0
22	1.12249319207879E-4	0.0	0.0	3.7820163120710673	0.0
23	1.12249319207879E-4	0.0	0.0	3.9369203725779403	0.0
24	1.12249319207879E-4	0.0	0.0	4.064248516999411	0.0
25	1.12249319207879E-4	0.0	0.0	4.17455218134102	0.0
26	1.12249319207879E-4	0.0	0.0	4.283022440135567	0.0
27	1.12249319207879E-4	0.0	0.0	4.394373764789783	0.0
28	1.12249319207879E-4	0.0	0.0	4.515191448697197	0.0
29	1.12249319207879E-4	0.0	0.0	4.649740965987708	0.0
30	1.12249319207879E-4	0.0	0.0	4.819985766786324	0.0
31	1.4966575894383868E-4	0.0	0.0	4.95801501297228	0.0
32	1.4966575894383868E-4	0.0	0.0	5.098139579783449	0.0
33	1.4966575894383868E-4	0.0	0.0	5.234896667018381	0.0
34	1.4966575894383868E-4	0.0	0.0	5.385722335594035	0.0
35	1.4966575894383868E-4	0.0	0.0	5.555630388435027	0.0
36	1.4966575894383868E-4	0.0	0.0	5.705408396698074	0.0
37	1.4966575894383868E-4	0.0	0.0	5.868693739705802	0.0
38	1.8708219867979834E-4	0.0	0.0	6.051922045092796	0.0
39	1.8708219867979834E-4	0.0	0.0	6.323565397575864	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	20650	0.0	43.53995	1
CGCCGGT	605	0.0	40.909092	28
CATACGA	410	0.0	40.609756	18
CGTAAGG	380	0.0	39.67105	2
CGGTCTA	205	0.0	39.512196	31
ACGCCGG	640	0.0	38.671875	27
TAAGGGA	3440	0.0	38.65552	4
AAGGGAT	4825	0.0	38.518135	5
TCGTCCC	635	0.0	38.267715	38
CTCGTCC	655	0.0	37.78626	37
CACGCCG	655	0.0	37.78626	26
GTTTTTT	24550	0.0	37.576378	2
GCGTTAG	180	0.0	37.5	1
CGGGCGA	390	0.0	37.5	6
AGGGATG	5510	0.0	37.486385	6
GGGCGAT	3465	0.0	37.467533	7
TTAGGGA	4050	0.0	37.38889	4
TAGGGAT	4735	0.0	37.349525	5
ATAGGGA	4170	0.0	37.338127	4
AGGGCGA	1900	0.0	37.30263	6
>>END_MODULE