FastQCFastQC Report
Thu 26 May 2016
SRR1548173_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548173_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1281684
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76990.6006940868419985No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31250.24381984951048777No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15980.12467971824568302No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT2050.045.00000428
CGACGAA253.891057E-545.00000443
CGCGATT253.891057E-545.00000442
TCTAGCG850.045.0000041
CGCTATA253.891057E-545.00000439
CGTCGTT253.891057E-545.00000427
TCGAACG502.1827873E-1145.00000428
TGTCCGA253.891057E-545.00000432
CTATGCG207.0335914E-445.01
AGTTTCG207.0335914E-445.034
TCCGATT207.0335914E-445.042
CGCATCG406.8157533E-945.021
GCTATCG207.0335914E-445.012
CCAATCG453.8562575E-1045.024
TACGCTA207.0335914E-445.033
CCGTAAT207.0335914E-445.023
TACGATC207.0335914E-445.042
GGGCGTA207.0335914E-445.07
CGCTAAG207.0335914E-445.033
GAAACGT207.0335914E-445.042