FastQCFastQC Report
Thu 26 May 2016
SRR1548172_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548172_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2213491
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91600.4138259428206394No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC35880.16209688677297537No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA406.8193913E-945.00000433
CGCGAAA207.034703E-445.00000442
TACGTTG750.042.0000041
CGTTTTT61850.041.1802751
TAGTGCG1450.040.3448261
TAGGGCG6700.039.962695
ACGGGAT9050.039.5303845
GCGATAA1950.039.230779
ACGGGTA2300.039.1304365
GCGAACC1100.038.8636369
AGGGCGA17350.038.7752156
CGTCATA5300.038.63207638
TTGTACG1750.038.5714261
CGTTCCG356.2509116E-638.57142621
TAGGGAC19350.037.5581365
TCGCATA301.14026356E-437.49999634
CCCGTAA301.14026356E-437.49999640
ATACGCG601.5643309E-1037.4999961
TACGGGA9550.037.4607354
TATGGGA20750.037.4096374
Produced by FastQC (version 0.11.5)