##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548172_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2213491
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.095884284146628	31.0	31.0	33.0	30.0	34.0
2	31.472511973168178	31.0	31.0	34.0	30.0	34.0
3	31.593157144076937	31.0	31.0	34.0	30.0	34.0
4	35.40053291384514	37.0	35.0	37.0	33.0	37.0
5	35.23835018981329	37.0	35.0	37.0	33.0	37.0
6	35.27888028458214	37.0	35.0	37.0	32.0	37.0
7	35.7305455499932	37.0	35.0	37.0	35.0	37.0
8	35.72635081868415	37.0	35.0	37.0	35.0	37.0
9	37.44905355386582	39.0	37.0	39.0	35.0	39.0
10	36.698095903710474	39.0	37.0	39.0	32.0	39.0
11	36.41968004387639	38.0	35.0	39.0	32.0	39.0
12	35.807527566183914	37.0	35.0	39.0	31.0	39.0
13	35.52112929304885	37.0	35.0	39.0	30.0	39.0
14	36.44312626525249	38.0	35.0	40.0	30.0	41.0
15	36.706916359723174	38.0	35.0	40.0	31.0	41.0
16	36.81508214851563	38.0	35.0	40.0	31.0	41.0
17	36.68487832116779	38.0	35.0	40.0	31.0	41.0
18	36.62589457106444	38.0	35.0	40.0	31.0	41.0
19	36.56318683925076	38.0	35.0	40.0	31.0	41.0
20	36.45968427249083	38.0	35.0	40.0	31.0	41.0
21	36.30015346798338	38.0	35.0	40.0	30.0	41.0
22	36.22970502251873	38.0	34.0	40.0	30.0	41.0
23	36.18115637244516	38.0	34.0	40.0	30.0	41.0
24	35.99863021805826	38.0	34.0	40.0	30.0	41.0
25	35.89569146655668	38.0	34.0	40.0	30.0	41.0
26	35.833562910352924	38.0	34.0	40.0	30.0	41.0
27	35.83249717301764	38.0	34.0	40.0	30.0	41.0
28	35.85365018425645	38.0	34.0	40.0	30.0	41.0
29	35.90049699772893	38.0	34.0	40.0	30.0	41.0
30	35.82326108396194	38.0	34.0	40.0	30.0	41.0
31	35.709357752075796	38.0	34.0	40.0	29.0	41.0
32	35.575822987308285	38.0	34.0	40.0	29.0	41.0
33	35.47373312111954	38.0	34.0	40.0	28.0	41.0
34	35.34434474773107	38.0	34.0	40.0	27.0	41.0
35	35.20243678424715	38.0	34.0	40.0	27.0	41.0
36	35.027485542069066	37.0	34.0	40.0	26.0	41.0
37	34.955872872308944	37.0	34.0	40.0	26.0	41.0
38	34.88003429876155	37.0	33.0	40.0	26.0	41.0
39	34.85623975882441	37.0	34.0	40.0	25.0	41.0
40	34.715069543991824	37.0	33.0	40.0	24.0	41.0
41	34.672886404326924	37.0	33.0	40.0	24.0	41.0
42	34.6223522029229	37.0	33.0	40.0	24.0	41.0
43	34.53044760516307	37.0	33.0	40.0	24.0	41.0
44	34.39025774218192	36.0	33.0	40.0	24.0	41.0
45	34.29290880333374	36.0	33.0	40.0	24.0	41.0
46	34.18007301588305	36.0	33.0	40.0	23.0	41.0
47	34.154201214280974	36.0	33.0	40.0	23.0	41.0
48	34.05614795813491	36.0	33.0	40.0	23.0	41.0
49	33.938736593010766	36.0	33.0	40.0	24.0	41.0
50	33.78281140515141	35.0	32.0	40.0	23.0	41.0
51	33.42300962597092	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	4.0
12	4.0
13	8.0
14	34.0
15	90.0
16	247.0
17	565.0
18	1294.0
19	2357.0
20	4076.0
21	6563.0
22	9652.0
23	13874.0
24	19186.0
25	26578.0
26	35083.0
27	42691.0
28	49465.0
29	56897.0
30	67799.0
31	82733.0
32	101306.0
33	127756.0
34	194598.0
35	250792.0
36	174179.0
37	215823.0
38	304165.0
39	425612.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.48199744204969	23.77624304774675	29.79709427325433	12.944665236949234
2	30.39090739469914	25.880701570505593	30.414535229644034	13.313855805151228
3	29.06725168523387	25.56942856329662	31.304441716727105	14.058878034742406
4	26.13455396927297	28.357693796812367	30.389145472016825	15.118606761897835
5	23.221463290340914	32.28208291788853	29.883699549715807	14.612754242054745
6	21.63270598344425	41.095039464809204	27.039820807945457	10.232433743801082
7	88.66663564478013	3.8101803892584156	5.988820374693189	1.5343635912682725
8	89.49943776595433	2.814739251255144	5.781862225778194	1.9039607570123394
9	84.62401699397016	5.262863052074755	7.350063768047849	2.7630561859072387
10	43.027145807233914	30.42203469542004	14.091857613154968	12.45896188419108
11	37.12596979160973	23.901158848172415	24.048347158402724	14.924524201815142
12	34.59625541734753	22.276124005021934	26.375304891684674	16.752315685945867
13	23.750085272540073	30.98837989402261	27.189810123465602	18.071724709971715
14	18.503350589634202	34.57357631000081	28.167586857141053	18.75548624322394
15	17.57743763132536	26.308668072289425	39.842764212730025	16.271130083655187
16	20.851632105122633	23.788757216541654	37.935957272923176	17.423653405412537
17	21.427374224697548	23.574435134364677	28.522682043884522	26.475508597053256
18	23.297768095736554	25.191066961645653	32.05637610453352	19.454788838084276
19	27.404764690708028	26.065613097139316	27.047817226272887	19.481804985879773
20	29.232239932306026	26.604354840385618	26.185514194546084	17.977891032762276
21	24.199240024016362	27.248179459505366	29.340711121030083	19.211869395448186
22	24.2408485058218	23.97999359382984	28.727516850079805	23.051641050268557
23	21.514115033673054	27.989948908759963	28.15805440365468	22.3378816539123
24	21.380344442331143	25.335770509118856	34.46560207382817	18.818282974721832
25	20.361998309457775	26.901125868594	32.111221595208654	20.62565422673957
26	19.305115765096854	32.02877264917725	28.122002754924235	20.54410883080166
27	19.852983364287454	30.80699221275352	30.40062055820421	18.939403864754816
28	17.976174287584634	28.685863190769695	34.71403317203458	18.62392934961109
29	19.094317528284506	26.486938505735964	34.054441603783346	20.364302362196188
30	20.606860384794878	28.5009064866313	31.50164152463236	19.390591603941466
31	26.593873659301074	26.836476859404446	27.19979435199872	19.36985512929576
32	26.772324802766306	27.151996552052843	28.454373656816312	17.621304988364532
33	25.34173393973592	27.58321583417326	27.52326528546988	19.551784940620948
34	20.74632334172581	27.74946905137631	29.754717773869423	21.74948983302846
35	21.03279389886835	27.001419928971927	30.379567841025786	21.586218331133942
36	25.794728779109562	26.49597400667091	28.683197717993885	19.026099496225648
37	21.269027070812577	30.01105493539391	29.708456009082486	19.01146198471103
38	21.28551686001886	30.40405404855949	27.31151832105936	20.998910770362293
39	21.133946331835098	28.962846471930536	29.375362267115612	20.527844929118753
40	22.906711615271984	26.50595823520403	28.728194512649928	21.85913563687406
41	18.991990480196215	25.75357207235087	29.400119539677373	25.85431790777555
42	22.108199220145913	25.72709805461147	28.12430680766265	24.040395917579968
43	21.537788046122618	26.132837224095333	28.9689906125663	23.360384117215744
44	20.28876557438002	29.233459724932242	29.070956240617196	21.40681846007054
45	19.745686790684942	31.634689275899476	26.786510539234175	21.833113394181407
46	21.700201175428315	29.66580844466953	28.26254997196736	20.371440407934795
47	21.092111962506284	27.85057630683838	29.02505589586766	22.032255834787673
48	21.783553671553214	25.940832829227677	30.715101168245095	21.560512330974014
49	20.997103670175303	25.256122568377283	31.131411873822845	22.615361887624573
50	20.068886659128047	29.705474293773953	29.217828308314786	21.007810738783217
51	19.41453568141908	30.935657746067186	26.96929872314818	22.68050784936555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	975.0
1	1983.0
2	2991.0
3	9502.5
4	16014.0
5	11914.0
6	7814.0
7	7938.0
8	8062.0
9	8714.5
10	9367.0
11	9526.0
12	9685.0
13	9543.5
14	9402.0
15	8776.0
16	8150.0
17	8194.0
18	8238.0
19	7776.0
20	7314.0
21	8422.0
22	9530.0
23	11293.0
24	13056.0
25	14642.5
26	18242.5
27	20256.0
28	26266.0
29	32276.0
30	38010.0
31	43744.0
32	48277.5
33	52811.0
34	61192.0
35	69573.0
36	77669.0
37	85765.0
38	95257.0
39	104749.0
40	122094.5
41	139440.0
42	164357.0
43	189274.0
44	192031.5
45	194789.0
46	195525.0
47	196261.0
48	189273.0
49	182285.0
50	175764.5
51	169244.0
52	153350.0
53	137456.0
54	123575.0
55	109694.0
56	101854.5
57	94015.0
58	85345.0
59	76675.0
60	68731.5
61	60788.0
62	52916.5
63	45045.0
64	37273.0
65	29501.0
66	25252.5
67	21004.0
68	17278.0
69	13552.0
70	11027.5
71	8503.0
72	6736.0
73	4969.0
74	3714.5
75	1863.5
76	1267.0
77	893.5
78	520.0
79	386.5
80	253.0
81	263.5
82	274.0
83	203.5
84	133.0
85	84.5
86	36.0
87	28.5
88	21.0
89	17.0
90	13.0
91	12.5
92	12.0
93	8.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2213491.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.08038312891812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.5182027250179	17.18434945424194
2	7.246800883038802	3.0553067814688015
3	2.2123548309594665	1.3991186236121531
4	1.0154444877757263	0.856238353938413
5	0.5999480784946599	0.6323567676062836
6	0.4186690335445669	0.5295422178800006
7	0.3219957216699112	0.4751455225071947
8	0.24818919493733205	0.4185538654189363
9	0.20582474567372946	0.39049780465929146
>10	3.3881044446394455	18.73300439249164
>50	1.833644030011065	27.745291390109646
>100	0.9888807280149005	27.806360895438214
>500	0.0015097415064501856	0.19463829097518462
>1k	2.1567735806431225E-4	0.1631306208874007
>5k	2.1567735806431225E-4	0.416465018764936
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9160	0.4138259428206394	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3588	0.16209688677297537	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5177504674742295E-5	0.0	0.0	0.03980138161844796	0.0
2	4.5177504674742295E-5	0.0	0.0	0.1397340219589779	0.0
3	4.5177504674742295E-5	0.0	0.0	0.20795205401783878	0.0
4	4.5177504674742295E-5	0.0	0.0	0.33395211455569507	0.0
5	4.5177504674742295E-5	0.0	0.0	0.562911708247289	0.0
6	4.5177504674742295E-5	0.0	0.0	0.8632969368296506	0.0
7	4.5177504674742295E-5	0.0	0.0	1.0275623438270136	0.0
8	4.5177504674742295E-5	0.0	0.0	1.449972012535854	0.0
9	4.5177504674742295E-5	0.0	0.0	1.5913324246631226	0.0
10	4.5177504674742295E-5	0.0	0.0	1.844733048383752	0.0
11	4.5177504674742295E-5	0.0	0.0	2.16327963384536	0.0
12	4.5177504674742295E-5	0.0	0.0	2.413834074771481	0.0
13	4.5177504674742295E-5	0.0	0.0	2.5231184585796824	0.0
14	4.5177504674742295E-5	0.0	0.0	2.567211703142231	0.0
15	4.5177504674742295E-5	0.0	0.0	2.6399926631732407	0.0
16	4.5177504674742295E-5	0.0	0.0	2.798972302123659	0.0
17	4.5177504674742295E-5	0.0	0.0	2.9857361064490435	0.0
18	4.5177504674742295E-5	0.0	0.0	3.2026333063924812	0.0
19	4.5177504674742295E-5	0.0	0.0	3.321088723649656	0.0
20	4.5177504674742295E-5	0.0	0.0	3.451154759608239	0.0
21	4.5177504674742295E-5	0.0	0.0	3.629018595512699	0.0
22	4.5177504674742295E-5	0.0	0.0	3.8161889973801566	0.0
23	4.5177504674742295E-5	0.0	0.0	4.019849188453895	0.0
24	4.5177504674742295E-5	0.0	0.0	4.1762988871425275	0.0
25	4.5177504674742295E-5	0.0	0.0	4.305190307979567	0.0
26	4.5177504674742295E-5	0.0	0.0	4.436656846583067	0.0
27	4.5177504674742295E-5	0.0	0.0	4.569523887831484	0.0
28	4.5177504674742295E-5	0.0	0.0	4.705282289379085	0.0
29	4.5177504674742295E-5	0.0	0.0	4.861641633058368	0.0
30	4.5177504674742295E-5	0.0	0.0	5.048766857421151	0.0
31	4.5177504674742295E-5	0.0	0.0	5.221344925278666	0.0
32	4.5177504674742295E-5	0.0	0.0	5.380595629257133	0.0
33	4.5177504674742295E-5	0.0	0.0	5.541969675955312	0.0
34	4.5177504674742295E-5	0.0	0.0	5.716490376513842	0.0
35	4.5177504674742295E-5	0.0	0.0	5.916084592166853	0.0
36	4.5177504674742295E-5	0.0	0.0	6.092683457940421	0.0
37	4.5177504674742295E-5	0.0	0.0	6.277323919546093	0.0
38	4.5177504674742295E-5	0.0	0.0	6.478544525367394	0.0
39	4.5177504674742295E-5	0.0	0.0	6.745859820527845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	40	6.8193913E-9	45.000004	33
CGCGAAA	20	7.034703E-4	45.000004	42
TACGTTG	75	0.0	42.000004	1
CGTTTTT	6185	0.0	41.180275	1
TAGTGCG	145	0.0	40.344826	1
TAGGGCG	670	0.0	39.96269	5
ACGGGAT	905	0.0	39.530384	5
GCGATAA	195	0.0	39.23077	9
ACGGGTA	230	0.0	39.130436	5
GCGAACC	110	0.0	38.863636	9
AGGGCGA	1735	0.0	38.775215	6
CGTCATA	530	0.0	38.632076	38
TTGTACG	175	0.0	38.571426	1
CGTTCCG	35	6.2509116E-6	38.571426	21
TAGGGAC	1935	0.0	37.558136	5
TCGCATA	30	1.14026356E-4	37.499996	34
CCCGTAA	30	1.14026356E-4	37.499996	40
ATACGCG	60	1.5643309E-10	37.499996	1
TACGGGA	955	0.0	37.460735	4
TATGGGA	2075	0.0	37.409637	4
>>END_MODULE