FastQCFastQC Report
Thu 26 May 2016
SRR1548171_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548171_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1932815
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108150.5595465680885134No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC26310.13612270186230963No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG1850.042.5675662
CGTTTTT64750.041.5250971
CGGTCTA1100.040.90909231
CGCGTAA556.184564E-1140.90909231
TACGGGA7350.040.7142874
GGGCGAT27050.039.9260647
GGCGATA6300.039.6428578
TACGGGT1200.039.3750044
GCGTTAG1150.039.1304321
TATTGCG1150.039.1304321
ACGGGAT9550.038.6387445
TCGAATA356.2504987E-638.5714317
GCGTAAG1650.038.181821
CCGTCGA301.14020964E-437.50000413
TTACGCG301.14020964E-437.5000041
AGGGATC21600.037.3958326
AGGGCGA15050.037.3754166
ACGGGAC6600.037.1590925
TGGGCGA8000.037.1256
ACATACG4350.036.72413617
Produced by FastQC (version 0.11.5)