##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548171_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1932815
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.104694448252936	31.0	31.0	33.0	30.0	34.0
2	31.49162025336103	31.0	31.0	34.0	30.0	34.0
3	31.6015293755481	31.0	31.0	34.0	30.0	34.0
4	35.41608120797903	37.0	35.0	37.0	33.0	37.0
5	35.26980181755626	37.0	35.0	37.0	33.0	37.0
6	35.30888108794686	37.0	35.0	37.0	32.0	37.0
7	35.730744018439424	37.0	35.0	37.0	35.0	37.0
8	35.72804639864653	37.0	35.0	37.0	35.0	37.0
9	37.43190786495345	39.0	37.0	39.0	35.0	39.0
10	36.741732136805645	39.0	37.0	39.0	32.0	39.0
11	36.453251345834964	39.0	35.0	39.0	32.0	39.0
12	35.89902499721908	37.0	35.0	39.0	31.0	39.0
13	35.687161989119495	37.0	35.0	39.0	30.0	39.0
14	36.58738420386845	38.0	35.0	41.0	31.0	41.0
15	36.830503178007206	38.0	35.0	41.0	31.0	41.0
16	36.93412975375295	38.0	35.0	41.0	31.0	41.0
17	36.80614181905666	38.0	35.0	41.0	31.0	41.0
18	36.744438552060075	38.0	35.0	40.0	31.0	41.0
19	36.68689295147234	38.0	35.0	40.0	31.0	41.0
20	36.553258330466186	38.0	35.0	40.0	31.0	41.0
21	36.41687228213771	38.0	35.0	40.0	30.0	41.0
22	36.35835297221928	38.0	35.0	40.0	30.0	41.0
23	36.281263338705465	38.0	35.0	40.0	30.0	41.0
24	36.123965304491115	38.0	34.0	40.0	30.0	41.0
25	35.98328758831031	38.0	34.0	40.0	30.0	41.0
26	35.92223984188865	38.0	34.0	40.0	30.0	41.0
27	35.90384491014401	38.0	34.0	40.0	30.0	41.0
28	35.90822608475203	38.0	34.0	40.0	30.0	41.0
29	35.92676484816188	38.0	34.0	40.0	30.0	41.0
30	35.841835354133735	38.0	34.0	40.0	29.0	41.0
31	35.72250163621454	38.0	34.0	40.0	29.0	41.0
32	35.58519206442417	38.0	34.0	40.0	29.0	41.0
33	35.452415259608394	38.0	34.0	40.0	27.0	41.0
34	35.301996311079954	38.0	34.0	40.0	27.0	41.0
35	35.140129293284666	38.0	34.0	40.0	26.0	41.0
36	34.962004640899416	38.0	34.0	40.0	25.0	41.0
37	34.880965327773225	37.0	33.0	40.0	25.0	41.0
38	34.794936401052354	37.0	33.0	40.0	25.0	41.0
39	34.75318331035304	37.0	33.0	40.0	24.0	41.0
40	34.61175228876018	37.0	33.0	40.0	24.0	41.0
41	34.540259673067524	37.0	33.0	40.0	24.0	41.0
42	34.49600918867041	37.0	33.0	40.0	24.0	41.0
43	34.396551661695504	37.0	33.0	40.0	23.0	41.0
44	34.250241745847376	37.0	33.0	40.0	23.0	41.0
45	34.165964150733515	36.0	33.0	40.0	23.0	41.0
46	34.0637598528571	36.0	33.0	40.0	23.0	41.0
47	34.026972058888205	36.0	33.0	40.0	23.0	41.0
48	33.89582344921785	36.0	33.0	40.0	23.0	41.0
49	33.78525984121605	36.0	32.0	40.0	23.0	41.0
50	33.629783502301045	35.0	32.0	40.0	23.0	41.0
51	33.274105385150676	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	6.0
13	13.0
14	30.0
15	92.0
16	195.0
17	538.0
18	1154.0
19	2157.0
20	3791.0
21	6127.0
22	8926.0
23	12538.0
24	17833.0
25	24223.0
26	32197.0
27	37927.0
28	42921.0
29	49859.0
30	59176.0
31	71211.0
32	86422.0
33	109543.0
34	163951.0
35	206462.0
36	157121.0
37	193277.0
38	269274.0
39	375796.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.442724730509646	23.066201369505098	29.436961116299283	14.054112783685971
2	29.752614709633356	25.93481528237312	30.58761443800881	13.724955569984711
3	28.120849641584943	26.11046582316466	31.95168704713074	13.816997488119659
4	25.8220781606103	28.02016747593536	31.164389763117526	14.993364600336815
5	23.16103714012981	32.54579460527779	29.768601754435885	14.524566500156508
6	23.10743656273363	39.994670985065824	26.54687592966735	10.351016522533197
7	88.16917294205602	3.8849036250236058	6.290876260790608	1.6550471721297693
8	89.06098100438997	2.779676275277251	6.208147184288201	1.9511955360445774
9	83.45402948549136	5.8155074334584524	7.947941215274095	2.782521865776083
10	40.714139739188695	36.03495419892747	13.350475860338417	9.900430201545413
11	35.471941184231284	21.8305424988941	26.725475536975864	15.97204077989875
12	32.88302294839392	21.091051135261264	28.696848896557615	17.3290770197872
13	24.169617888934017	29.55440639688744	29.071069916158553	17.204905798019986
14	18.855865667433253	32.29320964499965	30.516422937528944	18.334501750038157
15	17.557914233902366	26.519454784860425	39.0976891218249	16.824941859412306
16	21.861740518363113	24.748824900469003	35.86903040384103	17.52040417732685
17	21.779166655887916	24.389400951461987	28.79365071152697	25.03778168112313
18	23.286553550132837	24.71069398778466	32.567317617050776	19.43543484503173
19	26.19128059333149	25.856173508587215	28.2388123022638	19.713733595817498
20	27.387773791076746	26.020545163401565	28.142217439330718	18.449463606190967
21	24.366222323398773	26.251193207834167	30.21722203107902	19.165362437688035
22	24.15818378892962	23.426970506747928	29.94094106264697	22.473904641675485
23	21.496314960304012	27.047751595470853	29.317911957429963	22.138021486795168
24	21.27860141813883	25.35074489798558	33.83008720441429	19.540566479461305
25	20.230182402350973	27.193290614983844	31.517812103072462	21.058714879592717
26	20.17409840051945	30.966595354444166	28.2164614823457	20.642844762690686
27	19.834748799031466	29.605833977902694	31.022472404239416	19.536944818826427
28	18.116684731854836	27.834272809348022	34.434335412338996	19.614707046458147
29	19.240537764866268	25.864555066056504	33.78848984512227	21.106417323954958
30	21.28765556972602	27.637047518774434	31.36772013876134	19.707576772738207
31	25.79382920765826	26.501036053631623	27.842395676771964	19.862739061938157
32	26.21000975261471	27.068188109053377	27.8918054754335	18.829996662898417
33	24.853025250735328	26.983130822142833	27.662295667200432	20.50154825992141
34	21.025809505824405	26.909921539309245	29.73812806709385	22.326140887772496
35	20.92502386415668	27.71662057672359	30.23300212384527	21.12535343527446
36	25.6574478157506	26.655111844641105	28.21625453030942	19.47118580929887
37	21.150808535736736	30.067647446858597	29.117323696266844	19.664220321137822
38	21.107452084136348	29.84129365717878	27.443495626844783	21.60775863184009
39	20.97645144517194	29.10723478449826	28.14325219951211	21.773061570817692
40	23.526048794116353	26.57864306723613	27.77932704371603	22.115981094931485
41	19.803240351508034	26.08640764894726	29.244081818487544	24.866270181057164
42	22.3411449104027	26.609013278560028	27.92636646549204	23.123475345545227
43	22.287906499070008	26.38260775087114	28.572988102844814	22.75649764721404
44	20.858540522502153	28.71402591556874	28.62312223363333	21.804311328295775
45	19.41116971877805	30.944554962580483	27.1672663964218	22.477008922219664
46	21.536825821405568	29.314859414894855	28.079717924374552	21.068596839325025
47	21.337737962505464	27.524465611038824	29.237562829344764	21.900233597110947
48	21.568386006938066	26.485411174892583	30.832180006881156	21.114022811288198
49	22.203418330259233	25.18973621376076	30.807449238545853	21.799396217434158
50	20.27695356254996	29.302752720772553	29.361630575093837	21.05866314158365
51	19.586199403460753	29.996559422396867	27.894081947832568	22.523159226309815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	870.0
1	1625.0
2	2380.0
3	10006.0
4	17632.0
5	12507.0
6	7382.0
7	7465.0
8	7548.0
9	8210.5
10	8873.0
11	9194.0
12	9515.0
13	9291.5
14	9068.0
15	8660.0
16	8252.0
17	7845.5
18	7439.0
19	7926.5
20	8414.0
21	9209.5
22	10005.0
23	11607.5
24	13210.0
25	13760.0
26	17703.5
27	21097.0
28	26657.5
29	32218.0
30	36270.0
31	40322.0
32	46218.5
33	52115.0
34	58206.0
35	64297.0
36	69156.0
37	74015.0
38	81612.5
39	89210.0
40	105693.5
41	122177.0
42	135308.5
43	148440.0
44	152307.5
45	156175.0
46	156174.5
47	156174.0
48	151309.0
49	146444.0
50	142830.5
51	139217.0
52	127362.0
53	115507.0
54	106906.5
55	98306.0
56	91287.5
57	84269.0
58	77635.0
59	71001.0
60	64149.0
61	57297.0
62	50439.5
63	43582.0
64	37912.0
65	32242.0
66	27838.5
67	23435.0
68	19697.0
69	15959.0
70	13123.5
71	10288.0
72	8174.0
73	6060.0
74	5035.5
75	3086.0
76	2161.0
77	1609.0
78	1057.0
79	778.0
80	499.0
81	339.5
82	180.0
83	128.5
84	77.0
85	60.0
86	43.0
87	31.0
88	19.0
89	17.0
90	15.0
91	9.5
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1932815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.481908663515927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.0673327664453	18.225483708500754
2	6.313026581284248	2.8385777398156136
3	2.038939893485808	1.375177813672405
4	1.0939034311902578	0.9837214810690421
5	0.6744872106208578	0.7581879931943879
6	0.49348941760740117	0.6656750407836757
7	0.3825998376378838	0.602110222311565
8	0.3284214071008107	0.5906832062067047
9	0.2809838529514207	0.5685347986178969
>10	4.90059336093122	29.23726706582521
>50	2.024537090629724	32.06214685064436
>100	0.400528222880111	11.278479253941217
>500	6.941563410236754E-4	0.11449572255021799
>1k	2.3138544700789174E-4	0.13686426443872038
>5k	0.0	0.0
>10k+	2.3138544700789174E-4	0.5625948384282633
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10815	0.5595465680885134	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2631	0.13612270186230963	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.173800906967299E-5	0.0	0.0	0.03973479096550886	0.0
2	5.173800906967299E-5	0.0	0.0	0.11692790049746096	0.0
3	5.173800906967299E-5	0.0	0.0	0.17740963309990868	0.0
4	5.173800906967299E-5	0.0	0.0	0.2872494263548244	0.0
5	5.173800906967299E-5	0.0	0.0	0.5066703228193076	0.0
6	5.173800906967299E-5	0.0	0.0	0.8120280523485176	0.0
7	5.173800906967299E-5	0.0	0.0	0.9861781908770368	0.0
8	5.173800906967299E-5	0.0	0.0	1.4181905666088064	0.0
9	5.173800906967299E-5	0.0	0.0	1.595755413735924	0.0
10	5.173800906967299E-5	0.0	0.0	1.8826426740272608	0.0
11	5.173800906967299E-5	0.0	0.0	2.1658565356746506	0.0
12	5.173800906967299E-5	0.0	0.0	2.4153372154086137	0.0
13	5.173800906967299E-5	0.0	0.0	2.5237800824186483	0.0
14	5.173800906967299E-5	0.0	0.0	2.5732416190892557	0.0
15	5.173800906967299E-5	0.0	0.0	2.643243145360523	0.0
16	5.173800906967299E-5	0.0	0.0	2.763068374365886	0.0
17	5.173800906967299E-5	0.0	0.0	2.9158507151486304	0.0
18	5.173800906967299E-5	0.0	0.0	3.095174654584117	0.0
19	5.173800906967299E-5	0.0	0.0	3.2023758093764796	0.0
20	5.173800906967299E-5	0.0	0.0	3.318889805801383	0.0
21	5.173800906967299E-5	0.0	0.0	3.467119201785996	0.0
22	5.173800906967299E-5	0.0	0.0	3.631490856600347	0.0
23	5.173800906967299E-5	0.0	0.0	3.794620799197026	0.0
24	5.173800906967299E-5	0.0	0.0	3.9244314639528355	0.0
25	5.173800906967299E-5	0.0	0.0	4.037272061733792	0.0
26	5.173800906967299E-5	0.0	0.0	4.144059312453598	0.0
27	5.173800906967299E-5	0.0	0.0	4.266574917930583	0.0
28	5.173800906967299E-5	0.0	0.0	4.383658032455253	0.0
29	5.173800906967299E-5	0.0	0.0	4.523040228888952	0.0
30	5.173800906967299E-5	0.0	0.0	4.701898526242812	0.0
31	5.173800906967299E-5	0.0	0.0	4.848161877882777	0.0
32	5.173800906967299E-5	0.0	0.0	4.993597421377628	0.0
33	5.173800906967299E-5	0.0	0.0	5.134428282065278	0.0
34	5.173800906967299E-5	0.0	0.0	5.299369054979396	0.0
35	5.173800906967299E-5	0.0	0.0	5.475692189888841	0.0
36	5.173800906967299E-5	0.0	0.0	5.628888434744143	0.0
37	1.0347601813934598E-4	0.0	0.0	5.787155004488272	0.0
38	1.0347601813934598E-4	0.0	0.0	5.973670527184443	0.0
39	1.0347601813934598E-4	0.0	0.0	6.280528658976674	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	185	0.0	42.567566	2
CGTTTTT	6475	0.0	41.525097	1
CGGTCTA	110	0.0	40.909092	31
CGCGTAA	55	6.184564E-11	40.909092	31
TACGGGA	735	0.0	40.714287	4
GGGCGAT	2705	0.0	39.926064	7
GGCGATA	630	0.0	39.642857	8
TACGGGT	120	0.0	39.375004	4
GCGTTAG	115	0.0	39.130432	1
TATTGCG	115	0.0	39.130432	1
ACGGGAT	955	0.0	38.638744	5
TCGAATA	35	6.2504987E-6	38.57143	17
GCGTAAG	165	0.0	38.18182	1
CCGTCGA	30	1.14020964E-4	37.500004	13
TTACGCG	30	1.14020964E-4	37.500004	1
AGGGATC	2160	0.0	37.395832	6
AGGGCGA	1505	0.0	37.375416	6
ACGGGAC	660	0.0	37.159092	5
TGGGCGA	800	0.0	37.125	6
ACATACG	435	0.0	36.724136	17
>>END_MODULE