FastQCFastQC Report
Thu 26 May 2016
SRR1548170_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1548170_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1327214
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73790.5559766548574684No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC48800.36768750178946275No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACA207.033681E-445.00000420
CTATCGT207.033681E-445.00000431
CGTCTAT207.033681E-445.00000443
AAGCGCC207.033681E-445.00000421
CACTGCG207.033681E-445.0000041
CCGCTAT207.033681E-445.00000414
TACGCAG207.033681E-445.00000414
CGTAGGT207.033681E-445.00000410
CTACGCG406.8157533E-945.0000041
CTACGAT207.033681E-445.00000431
GTGTCGA207.033681E-445.00000418
TCGTCTA207.033681E-445.00000422
CATACGT207.033681E-445.00000418
TCCCGAA406.8157533E-945.00000428
ATCGCAC207.033681E-445.00000440
ACGACGA207.033681E-445.00000434
GTCGGTA351.21212E-745.00000437
TACCCGT253.891134E-544.9999961
ACGTTAG502.1827873E-1144.9999961
CGTGCGC253.891134E-544.99999618