##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1548170_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1327214
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16606590949161	31.0	31.0	33.0	30.0	34.0
2	31.552392455173017	31.0	31.0	34.0	30.0	34.0
3	31.656703440439898	31.0	31.0	34.0	30.0	34.0
4	35.47118173858926	37.0	35.0	37.0	33.0	37.0
5	35.3061548476734	37.0	35.0	37.0	33.0	37.0
6	35.35892478530214	37.0	35.0	37.0	33.0	37.0
7	35.786323079774625	37.0	35.0	37.0	35.0	37.0
8	35.7978863996311	37.0	35.0	37.0	35.0	37.0
9	37.606612045985045	39.0	37.0	39.0	35.0	39.0
10	36.82598962940415	39.0	37.0	39.0	32.0	39.0
11	36.50015671926306	39.0	35.0	39.0	32.0	39.0
12	35.546879403020164	37.0	35.0	39.0	31.0	39.0
13	35.061648686647366	37.0	34.0	39.0	30.0	39.0
14	35.918073498320545	38.0	34.0	40.0	29.0	41.0
15	36.351491922176834	38.0	35.0	40.0	31.0	41.0
16	36.55120123808218	38.0	35.0	40.0	32.0	41.0
17	36.461787624301735	38.0	35.0	40.0	31.0	41.0
18	36.41181979695814	38.0	35.0	40.0	31.0	41.0
19	36.337393216165594	37.0	35.0	40.0	31.0	41.0
20	36.186813882312876	37.0	35.0	40.0	31.0	41.0
21	36.00306657404156	37.0	34.0	40.0	30.0	41.0
22	35.9086153401034	37.0	34.0	40.0	30.0	41.0
23	35.92956599312545	37.0	34.0	40.0	30.0	41.0
24	35.75043738236637	36.0	34.0	40.0	30.0	41.0
25	35.632906223110965	36.0	34.0	40.0	30.0	41.0
26	35.550799644970596	36.0	34.0	40.0	30.0	41.0
27	35.510802327281056	36.0	34.0	40.0	30.0	41.0
28	35.59720436945361	36.0	34.0	40.0	30.0	41.0
29	35.70145884537083	36.0	34.0	40.0	30.0	41.0
30	35.686248035358275	36.0	34.0	40.0	30.0	41.0
31	35.49820676997078	36.0	34.0	40.0	30.0	41.0
32	35.29214429624763	36.0	34.0	40.0	29.0	41.0
33	35.17008636135544	36.0	34.0	40.0	29.0	41.0
34	35.07981757275014	36.0	34.0	40.0	28.0	41.0
35	34.928159287047905	36.0	34.0	40.0	27.0	41.0
36	34.70096307000981	36.0	34.0	40.0	25.0	41.0
37	34.60531835860682	36.0	33.0	40.0	25.0	41.0
38	34.570663058105175	35.0	33.0	40.0	26.0	41.0
39	34.60186902790356	36.0	33.0	40.0	26.0	41.0
40	34.44661750102094	36.0	33.0	40.0	24.0	41.0
41	34.46334954272634	36.0	34.0	40.0	25.0	41.0
42	34.404606190109504	36.0	33.0	40.0	24.0	41.0
43	34.29703273172224	35.0	33.0	40.0	24.0	41.0
44	34.158415297005604	35.0	33.0	40.0	24.0	41.0
45	34.09594082039521	35.0	33.0	40.0	24.0	41.0
46	34.01496216887405	35.0	33.0	40.0	23.0	41.0
47	33.995436304921434	35.0	33.0	40.0	24.0	41.0
48	33.925061067770535	35.0	33.0	39.0	23.0	41.0
49	33.873279667031845	35.0	33.0	39.0	24.0	41.0
50	33.698668790413606	35.0	33.0	39.0	24.0	41.0
51	33.322309740554275	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	12.0
15	34.0
16	92.0
17	271.0
18	619.0
19	1246.0
20	2306.0
21	3623.0
22	5529.0
23	8083.0
24	11522.0
25	16423.0
26	21878.0
27	26153.0
28	29563.0
29	34188.0
30	40637.0
31	49937.0
32	62446.0
33	81653.0
34	137432.0
35	195943.0
36	92548.0
37	110075.0
38	158305.0
39	236649.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.639068002597924	24.07569540405692	31.519634361903957	13.765602231441198
2	29.03864787441965	26.53972908664315	31.29781632803753	13.123806710899673
3	27.413438978190406	25.493703351531856	33.08547076809015	14.00738690218759
4	24.195495225336682	28.08891407112945	32.951807319693735	14.763783383840135
5	22.059743191376825	32.89122929685793	31.818681840306084	13.230345671459162
6	20.357078813213242	40.72108943998481	28.39127676471164	10.530554982090305
7	89.41286032244989	3.9422429238992356	5.651311695024314	0.9935850586265665
8	91.81074039303383	2.0344872793686624	4.9396706183027	1.2151017092948084
9	89.14899933243622	3.14237191590806	6.150854346021064	1.5577744056346603
10	49.50196426499419	24.383482995206503	13.536023580221427	12.578529159577883
11	43.700187008274476	24.2203593391872	20.230573215773795	11.848880436764532
12	41.43612107768604	22.674640261480064	22.680667925443824	13.208570735390074
13	21.12296886560871	40.039511337282455	24.325994150152123	14.511525646956708
14	14.559219538069973	43.72829099150552	27.742398739012696	13.970090731411814
15	13.547928216549856	24.68750329637873	48.890081026872835	12.87448746019858
16	15.322773870679484	20.668256965342437	47.90305105280686	16.10591811117122
17	16.571479806572263	20.8146538538623	28.290313393318634	34.3235529462468
18	21.060808580982417	25.03966956346151	34.989911197440655	18.90961065811542
19	27.59117971932183	25.850315020787907	27.417884380363677	19.140620879526587
20	30.569222446417832	25.33125780770848	25.930106222508204	18.169413523365485
21	21.89903060094303	28.291368234512294	30.235591246023624	19.57400991852105
22	22.135616411520676	23.909859299253927	28.616560705357237	25.337963583868163
23	18.995052794801744	30.329321420660122	26.788897645745145	23.88672813879299
24	19.21769963246319	24.673790360861172	39.1746169042822	16.933893102393434
25	16.11202112093453	25.74905026619671	37.35772829400534	20.781200318863423
26	16.592651976244976	36.28683844504353	28.095167772491852	19.025341806219647
27	16.984525479689033	36.45591441922704	30.003827566616987	16.555732534466934
28	13.91101962456695	31.067032144025003	39.0696602055132	15.952288025894845
29	14.270042359408505	25.78725058656705	39.72803180195507	20.214675252069373
30	17.25629777865514	30.954766902699944	33.845634539720045	17.943300778924876
31	27.496168666093034	28.233954735257466	26.598423464490278	17.671453134159222
32	28.509042249403638	27.60044725266611	27.92390677012147	15.966603727808778
33	26.847968752590013	29.15204330273792	26.52435854353556	17.475629401136516
34	19.178595162498286	28.904457005426405	29.075793353596328	22.841154478478977
35	18.13030905340058	28.964130878667643	31.530785540236916	21.374774527694857
36	28.666213587258728	24.98564662518629	28.60382726523379	17.74431252232119
37	19.488944510832464	31.867279881013914	31.140042223786068	17.503733384367553
38	18.80164012736454	33.641070693949885	25.198724546305268	22.35856463238031
39	19.482163388873232	32.20626063317596	29.038949257617837	19.27262672033297
40	22.74802707023886	28.447560076973268	26.485404765169747	22.319008087618123
41	16.726315424641392	26.12690945092502	29.668840141830934	27.477934982602655
42	22.150986954628266	25.953388074568227	26.656891804938766	25.238733165864737
43	22.60065068632489	26.486911681160684	27.411706024800825	23.5007316077136
44	18.565807774782364	31.802934568200758	29.79248259888759	19.838775058129286
45	16.623468408259708	37.51045422968715	24.06657856231173	21.799498799741414
46	21.943559968475316	33.25025203169948	26.816172825181166	17.99001517464403
47	21.229884555165935	28.686632298935965	27.257473173128073	22.826009972770027
48	23.018518490612667	25.468613200282697	30.92319701268974	20.589671296414895
49	20.39000492761529	24.212598721833857	33.00778924875717	22.389607101793683
50	18.646201742899034	33.379018003125346	28.0635225366821	19.911257717293516
51	17.450011829290528	35.08846350324816	25.181093629211265	22.280431038250047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	678.0
1	1132.5
2	1587.0
3	6258.0
4	10929.0
5	7783.5
6	4638.0
7	4974.0
8	5310.0
9	5944.0
10	6578.0
11	6782.0
12	6986.0
13	6964.5
14	6943.0
15	6573.5
16	6204.0
17	5894.5
18	5585.0
19	5468.0
20	5351.0
21	5597.5
22	5844.0
23	6253.5
24	6663.0
25	7913.0
26	10371.5
27	11580.0
28	14816.0
29	18052.0
30	23182.0
31	28312.0
32	33491.5
33	38671.0
34	41254.5
35	43838.0
36	46327.0
37	48816.0
38	56875.0
39	64934.0
40	80887.0
41	96840.0
42	122352.5
43	147865.0
44	144277.5
45	140690.0
46	135946.5
47	131203.0
48	122207.5
49	113212.0
50	105649.0
51	98086.0
52	86147.0
53	74208.0
54	66313.0
55	58418.0
56	49740.0
57	41062.0
58	35361.5
59	29661.0
60	27222.0
61	24783.0
62	19723.0
63	14663.0
64	12119.0
65	9575.0
66	7468.0
67	5361.0
68	3816.5
69	2272.0
70	1715.0
71	1158.0
72	937.0
73	716.0
74	601.0
75	321.5
76	157.0
77	113.0
78	69.0
79	48.0
80	27.0
81	25.0
82	23.0
83	15.5
84	8.0
85	6.0
86	4.0
87	3.0
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1327214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.42727730612362
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8270603917066	15.896769933746086
2	7.19771478164441	2.79664002066782
3	2.1264931048224676	1.2393591371083765
4	1.0196536851473568	0.7923637959027426
5	0.5945429336767353	0.57751752214671
6	0.42367253101600244	0.49384822482210844
7	0.33168050042752556	0.4510554341187575
8	0.24672867124115902	0.38346130524587235
9	0.19170463266404986	0.33518691536697554
>10	2.9975611441242394	15.258876479801083
>50	1.8025034389046033	25.386568726239776
>100	1.2363925097723567	35.04850729634278
>500	0.003511370333843865	0.41066251850448554
>1k	3.9015225931598503E-4	0.369884291307104
>5k	3.9015225931598503E-4	0.559298398679328
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7379	0.5559766548574684	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4880	0.36768750178946275	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.534579954702106E-5	0.0	0.0	0.03639202118121117	0.0
2	7.534579954702106E-5	0.0	0.0	0.11490234430920711	0.0
3	7.534579954702106E-5	0.0	0.0	0.16387711401477079	0.0
4	7.534579954702106E-5	0.0	0.0	0.27719719653349045	0.0
5	7.534579954702106E-5	0.0	0.0	0.45938333983818735	0.0
6	7.534579954702106E-5	0.0	0.0	0.6867769628710969	0.0
7	7.534579954702106E-5	0.0	0.0	0.8018299987793981	0.0
8	7.534579954702106E-5	0.0	0.0	1.1259676284306825	0.0
9	7.534579954702106E-5	0.0	0.0	1.220601952661741	0.0
10	7.534579954702106E-5	0.0	0.0	1.4245630320355271	0.0
11	7.534579954702106E-5	0.0	0.0	1.7114044909110362	0.0
12	7.534579954702106E-5	0.0	0.0	1.914762803888446	0.0
13	7.534579954702106E-5	0.0	0.0	2.0016365107661613	0.0
14	7.534579954702106E-5	0.0	0.0	2.033206400776363	0.0
15	7.534579954702106E-5	0.0	0.0	2.0848182734660727	0.0
16	7.534579954702106E-5	0.0	0.0	2.215543235680154	0.0
17	7.534579954702106E-5	0.0	0.0	2.3602825166099817	0.0
18	7.534579954702106E-5	0.0	0.0	2.554750025240843	0.0
19	7.534579954702106E-5	0.0	0.0	2.6551106302374747	0.0
20	7.534579954702106E-5	0.0	0.0	2.7575055718218766	0.0
21	7.534579954702106E-5	0.0	0.0	2.9112110028977995	0.0
22	7.534579954702106E-5	0.0	0.0	3.0720742849306895	0.0
23	7.534579954702106E-5	0.0	0.0	3.2402461095196404	0.0
24	7.534579954702106E-5	0.0	0.0	3.363662529177661	0.0
25	7.534579954702106E-5	0.0	0.0	3.468995956944396	0.0
26	7.534579954702106E-5	0.0	0.0	3.5735759267156615	0.0
27	7.534579954702106E-5	0.0	0.0	3.6728063447190884	0.0
28	7.534579954702106E-5	0.0	0.0	3.7776123518889944	0.0
29	7.534579954702106E-5	0.0	0.0	3.900953425747468	0.0
30	7.534579954702106E-5	0.0	0.0	4.066111418354538	0.0
31	1.5069159909404211E-4	0.0	0.0	4.217405783844956	0.0
32	1.5069159909404211E-4	0.0	0.0	4.357548971002416	0.0
33	1.5069159909404211E-4	0.0	0.0	4.483225764646846	0.0
34	1.5069159909404211E-4	0.0	0.0	4.620053736624237	0.0
35	1.5069159909404211E-4	0.0	0.0	4.789129710807752	0.0
36	1.5069159909404211E-4	0.0	0.0	4.950821796635659	0.0
37	1.5069159909404211E-4	0.0	0.0	5.112965957260848	0.0
38	1.5069159909404211E-4	0.0	0.0	5.273301818696909	0.0
39	1.5069159909404211E-4	0.0	0.0	5.467467944129583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	20	7.033681E-4	45.000004	20
CTATCGT	20	7.033681E-4	45.000004	31
CGTCTAT	20	7.033681E-4	45.000004	43
AAGCGCC	20	7.033681E-4	45.000004	21
CACTGCG	20	7.033681E-4	45.000004	1
CCGCTAT	20	7.033681E-4	45.000004	14
TACGCAG	20	7.033681E-4	45.000004	14
CGTAGGT	20	7.033681E-4	45.000004	10
CTACGCG	40	6.8157533E-9	45.000004	1
CTACGAT	20	7.033681E-4	45.000004	31
GTGTCGA	20	7.033681E-4	45.000004	18
TCGTCTA	20	7.033681E-4	45.000004	22
CATACGT	20	7.033681E-4	45.000004	18
TCCCGAA	40	6.8157533E-9	45.000004	28
ATCGCAC	20	7.033681E-4	45.000004	40
ACGACGA	20	7.033681E-4	45.000004	34
GTCGGTA	35	1.21212E-7	45.000004	37
TACCCGT	25	3.891134E-5	44.999996	1
ACGTTAG	50	2.1827873E-11	44.999996	1
CGTGCGC	25	3.891134E-5	44.999996	18
>>END_MODULE